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Product Specification Form (PSF) / Certificate of Analysis (CofA)
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Next Generation Sequencing (NGS)What is the recovery rate with sparQ PureMag Beads?The recovery rate depends on many factors, including the fragment size, sample volume, sample concentration, and elution volume. Up to 90% recovery rate can be expected under the optimal condition. The yield is also correlated with the ratio of beads to sample, and the lower ratio usually results in lower yield. When the ratio reaches to 1.8X, it generally produces maximal recovery.Is there a size limit for the DNA fragments that can be bound with sparQ PureMag Beads?sparQ PureMag Beads will bind DNA fragments 100 bp and above and exclude primers 50 bases and below. There will be some recovery for oligo or DNA between 50 and 100 bases.How do I dry my samples before elution?Air-dry the beads on the magnetic stand for 5 min or until the beads are dry and before they start to crack. Do not over-dry the beads as it can reduce the DNA recovery.Can I still use the sparQ PureMag Beads if it was accidentally frozen?sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.Can I still use the sparQ PureMag Beads if I left it at room temperature for some time?sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.