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PCR & qPCR
qPCR Instrumentation
Q
Real-Time Quantitative PCR
SYBR Green Detection
PerfeCTa SYBR Green SuperMix
PerfeCTa SYBR Green FastMix
Probe-based Detection
PerfeCTa MultiPlex qPCR SuperMix
PerfeCTa qPCR ToughMix
PerfeCTa Multiplex qPCR ToughMix
PerfeCTa FastMix II
PerfeCTa qPCR FastMix UNG
Multiplexed Pre-Amplification
PerfeCTa PreAmp SuperMix
Conventional PCR
AccuStart Taq DNA Polymerase HiFi
AccuStart II PCR SuperMix
AccuStart II Taq DNA Polymerase
AccuStart II PCR ToughMix
5PRIME HotMaster Taq DNA Polymerase
5PRIME HotMasterMix
AccuStart II GelTrack PCR SuperMix
AccuStart Long Range SuperMix
Cloning
repliQa HiFi Assembly Mix
Next Generation Sequencing (NGS)
PerfeCTa NGS Quantification Kit
sparQ HiFi PCR Master Mix
sparQ DNA Library Prep Kit
sparQ DNA Frag & Library Prep Kit
sparQ PureMag Beads
sparQ Fast Library Quant Kit (for Q)
Reverse Transcription
Conventional RT-PCR
qScript XLT 1-Step RT-PCR Kit
Quantitative RT-qPCR
qScript XLT 1-Step RT-qPCR ToughMix
qScript One-Step SYBR Green RT-qPCR
UltraPlex 1-Step ToughMix
qScript One-Step RT-qPCR Kit
Qscript lyo 1-step
First-Strand cDNA Synthesis
qScript cDNA SuperMix
qScript XLT cDNA SuperMix
qScript cDNA Synthesis Kit
qScript Flex cDNA Kit
PerfeCTa DNase I
Sample Preparation
Extracta DNA Prep
5PRIME Phase Lock Gel
Extracta DBS
Genotyping
AccuStart II PCR Genotyping Kit
AccuStart Genotyping ToughMix
AccuMelt HRM SuperMix
microRNA Profiling
qScript microRNA cDNA Synthesis Kit
PerfeCTa SYBR Green SuperMix
Materials
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Product Manuals
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CofA (PSF)
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FAQs
  • Next Generation Sequencing (NGS)
    What is the recovery rate with sparQ PureMag Beads?
    The recovery rate depends on many factors, including the fragment size, sample volume, sample concentration, and elution volume. Up to 90% recovery rate can be expected under the optimal condition. The yield is also correlated with the ratio of beads to sample, and the lower ratio usually results in lower yield. When the ratio reaches to 1.8X, it generally produces maximal recovery.
    Is there a size limit for the DNA fragments that can be bound with sparQ PureMag Beads?
    sparQ PureMag Beads will bind DNA fragments 100 bp and above and exclude primers 50 bases and below. There will be some recovery for oligo or DNA between 50 and 100 bases.
    How do I dry my samples before elution?
    Air-dry the beads on the magnetic stand for 5 min or until the beads are dry and before they start to crack. Do not over-dry the beads as it can reduce the DNA recovery.
    Can I still use the sparQ PureMag Beads if it was accidentally frozen?
    sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.
    Can I still use the sparQ PureMag Beads if I left it at room temperature for some time?
    sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.
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