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Conventional PCR

Home / Products / PCR / Conventional PCR

Conventional PCR

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.

Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensure specific and efficient primer extension only after activation at 94°C.  Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.

AccuStart II PCR SuperMix AccuStart II GelTrack PCR SuperMix sparQ HiFi PCR Master Mix AccuStart II PCR ToughMix repliQa HiFi ToughMix AccuStart Long Range SuperMix
Features
Concentration2x2x2x2x2x4x
Amplicon SizeUp to 4 kbUp to 4 kbUp to 4 kbUp to 4 kbUp to 24 kbUp to 24 kb
Extension Time*60 sec/kb60 sec/kb30 sec/kb60 sec/kb1 - 10 sec/kb30 - 60 sec/kb
Multiplex PCRUp to 6 Targets
Fidelity vs. Taq1x1x80x1x90x Taq10 - 12x Taq
dU TolerantNoNoNoNoYesYes
5’ → 3’ exoxxN/AxN/AN/A
3’ → 5’ exoN/AN/AxN/Axx
Resulting Ends3’ dA overhang3’ dA overhangBlunt3’ dA overhangBluntBlunt/T overhangs
Applications
Routine PCRxxxxx
High Fidelityx**x
Crude PCRx**
Fast PCR**
Long PCRx**
High Yield**x
NGS Library Amplification**x
Available Formats
Hot Startxxxxxx
Master Mixxxxxxx
Loading Dye Availablexx
Packaging (Rxns/Units)100 Rxns 500 Rxns 4000 Rxns100 Rxns 500 Rxns 4000 Rxns50 Rxns 250 Rxns100 Rxns 800 Rxns 4000 Rxns25 Rxns 100 Rxns 500 Rxns25 Rxns 100 Rxns

PCR Products

Showing 1–5 of 9 results

  • AccuStart II GelTrack® PCR SuperMix

    AccuStart II GelTrack® PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. This kit improves assay reproducibility, and reduces the risk…
    AccuStart II GelTrack® PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up…
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  • Placeholder

    AccuStart II PCR Genotyping Kit

    The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It…
    The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal…
    Details
    Details
  • AccuStart II PCR SuperMix

    AccuStart II PCR SuperMix

    AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart II PCR SuperMix simplifies reaction assembly, improves assay reproducibility,…
    AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to…
    Details
    Details
  • AccuStart II PCR ToughMix

    AccuStart II PCR ToughMix

    AccuStart II PCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. The only…
    AccuStart II PCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many…
    Details
    Details
  • AccuStart II Taq DNA Polymerase

    AccuStart II Taq DNA Polymerase

    AccuStart II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at…
    AccuStart II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that…
    Details
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