Conventional PCR
Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.
Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensure specific and efficient primer extension only after activation at 94°C. Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.
Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.
| AccuStart II PCR SuperMix | AccuStart II GelTrack PCR SuperMix | sparQ HiFi PCR Master Mix | AccuStart II PCR ToughMix | repliQa HiFi ToughMix | AccuStart Long Range SuperMix | |
|---|---|---|---|---|---|---|
| Features | ||||||
| Concentration | 2x | 2x | 2x | 2x | 2x | 4x |
| Amplicon Size | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 24 kb | Up to 24 kb |
| Extension Time* | 60 sec/kb | 60 sec/kb | 30 sec/kb | 60 sec/kb | 1 - 10 sec/kb | 30 - 60 sec/kb |
| Multiplex PCR | Up to 6 Targets | |||||
| Fidelity vs. Taq | 1x | 1x | 80x | 1x | 90x Taq | 10 - 12x Taq |
| dU Tolerant | No | No | No | No | Yes | Yes |
| 5’ → 3’ exo | x | x | N/A | x | N/A | N/A |
| 3’ → 5’ exo | N/A | N/A | x | N/A | x | x |
| Resulting Ends | 3’ dA overhang | 3’ dA overhang | Blunt | 3’ dA overhang | Blunt | Blunt/T overhangs |
| Applications | ||||||
| Routine PCR | x | x | x | x | x | |
| High Fidelity | x | ** | x | |||
| Crude PCR | x | ** | ||||
| Fast PCR | ** | |||||
| Long PCR | x | ** | ||||
| High Yield | ** | x | ||||
| NGS Library Amplification | ** | x | ||||
| Available Formats | ||||||
| Hot Start | x | x | x | x | x | x |
| Master Mix | x | x | x | x | x | x |
| Loading Dye Available | x | x | ||||
| Packaging (Rxns/Units) | 100 Rxns 500 Rxns 4000 Rxns | 100 Rxns 500 Rxns 4000 Rxns | 50 Rxns 250 Rxns | 100 Rxns 800 Rxns 4000 Rxns | 25 Rxns 100 Rxns 500 Rxns | 25 Rxns 100 Rxns |
PCR Products
Showing 1–5 of 8 results
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AccuStart II GelTrack PCR SuperMix
AccuStart II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. This kit improves assay reproducibility, and reduces the risk… -
AccuStart II PCR SuperMix
AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart II PCR SuperMix simplifies reaction assembly, improves assay reproducibility,… -
AccuStart II PCR ToughMix
AccuStart II PCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. The only… -
AccuStart II Taq DNA Polymerase
AccuStart II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at… -
AccuStart Long Range SuperMix
The AccuStart Long Range SuperMix is a 4x, ready-to-use solution that contains all the components for long range target amplifications, including a blend of two hot-start thermostable DNA polymerases (one with strong proof-reading activity) and an optimized buffer to ensure…