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Real-Time Quantitative PCR

Home / Products / PCR / Real-Time Quantitative PCR

Real-Time Quantitative PCR Reagents

PerfeCTa qPCR reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR.  Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs.

PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.

Quantabio quantitative real-time PCR reagents are optimized to work in series with high-performance qScript RT reagents (for RNA targets) and Extracta DNA Prep (for DNA targets).

 SYGR Green Detection Probe-based Detection
PerfeCTa SYBR Green SuperMixPerfeCTa SYBR Green FastMixAccuStart Genotyping ToughMixPerfeCTa qPCR FastMix UNGPerfeCTa FastMix II PerfeCTa qPCR ToughMixPerfeCTa qPCR ToughMix, UNGPerfeCTa Multiplex qPCR ToughMixPerfeCTa Multiplex qPCR SuperMix
Features
Concentration2x2x2x2x2x2x2x5x2x
Sample TypegDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNA
Fast CyclingNoYesYesYesYesYesYesYesYes
Multiplex CompatibilityN/AN/AUp to 2 targetsUp to 2 targetsUp to 2 targetsUp to 2 targetsUp to 2 targetsUp to 5 targetsUp to 4 targets
Carryover Contamination ControlN/AN/AN/AHeat-labile UNG & blend of dTTP/dUTPN/AN/AHeat-labile UNG & blend of dTTP/dUTPN/AN/A
Applications
Gene Expressionxxxxxxxx
microRNAxx
ChIPxx
SNP Genotypingx
Inhibitor Tolerantx**xx
High Yieldxxx**
Available Formats
Master Mixxxxxxxxxx
Packaging (Rxns/Units)250 Rxns 1250 Rxns 2000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1000 Rxns 5000 Rxns50 Rxns 200 Rxns 1000 Rxns

All Products

Showing 1–5 of 15 results

  • AccuStart Genotyping ToughMix

    Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. This proprietary formulation has been rigorously optimized to destabilize single base-pair mismatches to ensure precise…
    Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional…
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  • PerfeCTa FastMix II

    PerfeCTa FastMix II is an advanced 2X concentrated qPCR SuperMix reagent for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety…
    PerfeCTa FastMix II is an advanced 2X concentrated qPCR SuperMix reagent for both fast and conventional PCR cycling protocols or…
    Details
    Details
  • PerfeCTa MultiPlex qPCR SuperMix

    PerfeCTa Multiplex qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all the necessary components except: primers, probe(s), and DNA template for highly-multiplexed, real-time quantitative PCR. This reagent formulation pushes the boundary of multiplex qPCR by enabling unbiased…
    PerfeCTa Multiplex qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all the necessary components except: primers, probe(s),…
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    Details
  • PerfeCTa Multiplex qPCR ToughMix

    Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with…
    Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of…
    Details
    Details
  • PerfeCTa PreAmp SuperMix

    PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all the necessary components, except primers and…
    PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts…
    Details
    Details
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