Real-Time Quantitative PCR Reagents
PerfeCTa qPCR reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR. Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs.
PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.
Quantabio quantitative real-time PCR reagents are optimized to work in series with high-performance qScript RT reagents (for RNA targets) and Extracta DNA Prep (for DNA targets).
| SYGR Green Detection | Probe-based Detection | ||||||||
| PerfeCTa SYBR Green SuperMix | PerfeCTa SYBR Green FastMix | AccuStart Genotyping ToughMix | PerfeCTa qPCR FastMix UNG | PerfeCTa FastMix II | PerfeCTa qPCR ToughMix | PerfeCTa qPCR ToughMix, UNG | PerfeCTa Multiplex qPCR ToughMix | PerfeCTa Multiplex qPCR SuperMix | |
| Features | |||||||||
| Concentration | 2x | 2x | 2x | 2x | 2x | 2x | 2x | 5x | 2x |
| Sample Type | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA |
| Fast Cycling | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Multiplex Compatibility | N/A | N/A | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 5 targets | Up to 4 targets |
| Carryover Contamination Control | N/A | N/A | N/A | Heat-labile UNG & blend of dTTP/dUTP | N/A | N/A | Heat-labile UNG & blend of dTTP/dUTP | N/A | N/A |
| Applications | |||||||||
| Gene Expression | x | x | x | x | x | x | x | x | |
| microRNA | x | x | |||||||
| ChIP | x | x | |||||||
| SNP Genotyping | x | ||||||||
| Inhibitor Tolerant | x | ** | x | x | |||||
| High Yield | x | x | x | ** | |||||
| Available Formats | |||||||||
| Master Mix | x | x | x | x | x | x | x | x | x |
| Packaging (Rxns/Units) | 250 Rxns 1250 Rxns 2000 Rxns | 250 Rxns 1250 Rxns 5000 Rxns | 250 Rxns 1250 Rxns 5000 Rxns | 250 Rxns 1250 Rxns 5000 Rxns | 250 Rxns 1250 Rxns 5000 Rxns | 250 Rxns 1250 Rxns 5000 Rxns | 250 Rxns 1250 Rxns 5000 Rxns | 250 Rxns 1000 Rxns 5000 Rxns | 50 Rxns 200 Rxns 1000 Rxns |
All Products
Showing 1–5 of 15 results
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AccuStart Genotyping ToughMix
Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. This proprietary formulation has been rigorously optimized to destabilize single base-pair mismatches to ensure precise… -
PerfeCTa FastMix II
PerfeCTa FastMix II is an advanced 2X concentrated qPCR SuperMix reagent for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety… -
PerfeCTa MultiPlex qPCR SuperMix
PerfeCTa Multiplex qPCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all the necessary components except: primers, probe(s), and DNA template for highly-multiplexed, real-time quantitative PCR. This reagent formulation pushes the boundary of multiplex qPCR by enabling unbiased… -
PerfeCTa MultiPlex qPCR ToughMix
Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with… -
PerfeCTa PreAmp SuperMix
PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all the necessary components, except primers and…