PerfeCTa FastMix II
Convenient, 1-tube reagent solution supports robust and reliable probe-based DNA detection with fast or standard thermal cycling conditions
Features & Benefits
- 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
- Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot-start
- Inert AccuVue plate loading dye simplifies reaction setup and provides instant visual cue of reagent addition and mixing
- Supports efficient vortex mixing with proprietary anti-foaming technology
- Flexible – optimized for both fast and standard thermal cycling conditions
PerfeCTa FastMix II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Product
Kit Size
Order Info
PerfeCTa FastMix II
250 x 20 μL rxns (2 x 1.25 mL)
1250 x 20 μL rxns (10 x 1.25 mL)
5000 x 20 μL rxns (1 x 50 mL)
PerfeCTa FastMix II ROX
250 x 20 μL rxns (2 x 1.25 mL)
1250 x 20 μL rxns (10 x 1.25 mL)
5000 x 20 μL rxns (1 x 50 mL)
PerfeCTa FastMix II Low ROX
250 x 20 μL rxns (2 x 1.25 mL)
1250 x 20 μL rxns (10 x 1.25 mL)
5000 x 20 μL rxns (1 x 50 mL)
Description
PerfeCTa FastMix II is an advanced 2X concentrated qPCR SuperMix reagent for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. The kit is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. Inert AccuVue plate loading dye helps to minimize pipette error and provides visual confirmation of thorough mixing.
A key component of the kit is an ultra pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa FastMix II is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples.
Details
Contents
Storage & Handling
Single-tube, 2X concentrated reagent containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
- AccuStart II Taq DNA Polymerase.
- Inert AccuVue dye.
- Proprietary enzyme stabilizers and performance-enhancing additives.
- Titrated reference dye (if applicable).
Instrument Capability
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Resources
Flyers
Product Manuals
Technical Notes
CofA (PSFs)
Click here to see all CofA (PSFs)
SDSs
- SDS 95118-012 Perfecta FastMix II, 1250R
- SDS 95118-05K Perfecta FastMix II, 5000R
- SDS 95118-250 Perfecta FastMix II, 250R
- SDS 95119-012 Perfecta FastMix II, ROX, 1250R
- SDS 95119-05K Perfecta FastMix II, ROX, 5000R
- SDS 95119-250 Perfecta FastMix II, ROX, 250R
- SDS 95120-012 Perfecta FastMix II, LOW ROX 1250R
- SDS 95120-05K Perfecta FastMix II, LOW ROX, 5000R
- SDS 95120-250 Perfecta FastMix II, LOW ROX, 250R