COVID-19 Testing Solutions
To help address and fight the global pandemic caused by SARS-COV-2 since late 2019, Quantabio provides three essential RT-qPCR ToughTM master mixes to help research labs and testing providers eliminate extraction and purification while overcoming common PCR inhibitors to accurately detect COVID-19 at low limits of detection (LOD).
Our RT-qPCR master mixes being used for COVID-19 in these latest developments
- UltraPlex 1-Step ToughMix Added to SalivaDirectTM – Emergency Use Authorization (EUA)
- CDC Adds Two Quantabio RT-qPCR ToughMix Kits to COVID-19 Testing Protocol
- qScript XLT 1-Step RT-qPCR ToughMix and UltraPlex 1-Step ToughMix granted EUA
Reagents Manufactured for
+ 100 Million Tests
Quantabio proudly supports and partners with thousands of Testing labs in more than 37 countries around the world.
Determine which product is right for you.
|qScript XLT 1-Step RT-qPCR ToughMix||qScript 1-Step Virus ToughMix||UltraPlex 1-Step ToughMix|
|Recommended in CDC protocol||x||x|
|Detection purified RNA||x||x||x|
|Formulation||2X master mix||2X master mix||4X master mix|
|Taq Hot Start||Yes||Yes||Yes|
|RT Hot Start||No||Yes||Yes|
|Chemistry||1 tube SuperMix||1 tube SuperMix||1 tube SuperMix|
|Cycling Conditions||Fast or Standard qPCR Cycling||Fast or Standard qPCR Cycling||Fast or Standard qPCR Cycling|
|Multiplex Capabilty||Up to 4 targets||Up to 4 targets||Up to 5 targets|
"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."
"It is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."
"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."