To help address and fight the global pandemic caused by SARS-COV-2 since late 2019, Quantabio provides three essential RT-qPCR ToughTM master mixes to help research labs and testing providers eliminate extraction and purification while overcoming common PCR inhibitors to accurately detect COVID-19 at low limits of detection (LOD).
Our RT-qPCR master mixes being used for COVID-19 in these latest developments
"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."
Rhoel R. D., PhD, MPH Professor of Infectious Diseases UF Eminent Scholar Director | CDC Southeastern Regional Center of Excellence for Vector Borne Diseases
qScript XLT 1-Step RT-qPCR ToughMix
"It is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."
Olivia L. | Senior Research Engineer
qScript 1-Step Virus ToughMix
"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."
Guillermo C. | Project Coordinator Sick Kids Molecular Diagnostics Lab