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sparQ RNA-Seq HMR Kit

Ultra FAST RNA library prep with integrated rRNA & globin depletion
Features & Benefits
  • High quality directional RNA library prep in 5 hours
  • Simple workflow with 3 reaction tubes, 9 steps and 9 components
  • Efficient and integrated removal of rRNA and globin mRNA from
    human/mouse/rat (HMR) samples
  • Improved results for samples with limited quantity and/or poor quality RNA

sparQ RNA-Seq HMR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Order Info
Product
Kit Size
Order Info
sparQ RNA-Seq HMR Kit
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Description

sparQ RNA-Seq HMR Kit simultaneously depletes rRNA and globin mRNA while generating stranded RNA-seq libraries for Illumina® NGS platforms in 5 hours. In a single step and tube, RNA fragmentation and depletion of abundant ribosomal and globin transcripts (human, mouse, and rat) are integrated. The proprietary, highly optimized enzymes and streamlined workflow generate high quality, directional transcriptome NGS libraries from either intact or degraded RNA samples, with key improvements for low input and FFPE samples.

 

 

sparQ UDI Adapter Resources

Setting up sparQ Library Prep kits in Illumina Experiment Manager

Video Tutorial

Watch the step-by-step tutorial illustrating the sparQ RNA-Seq HMR Kit protocol


 

Featured Webinar

Simple. Fast. Elegant. Directional RNA-Seq Library Preparation with Integrated Ribo-globin Depletion


 

Customer Testimonial

Find out how sparQ RNA-Seq HMR Kit made a difference in a core lab.

Details

Details

Contents

 

 

Volume

Component Description

Cap Color

95216-024

95216-096

Frag Prime RG Depletion Mix

Blue

1 x 96 μl

1 x 384 μl

1st Strand Enzyme Mix

Brown

1 x 96 μl

1 x 384 μl

2nd Strand Buffer

Green

1 x 480 μl

2 x 960 μl

2nd Strand Enzyme Mix

Green

1 x 240 μl

1 x 960 μl

Rapid Ligation Buffer (5X)

Orange

1 x 480 μl

2 x 960 μl

T4 DNA Ligase

Orange

1 x 240 μl

1 x 960 μl

HiFi Plus Master Mix (2X)

White

1 x 600 μl

2 x 1.2 ml

Primer Mix

White

1 x 36 μl

1 x 144 μl

UDI Dilution Buffer

Clear

1 x 1.44 ml

1 x 5.76 ml

Details

Contents

 

 

Volume

Component Description

Cap Color

95216-024

95216-096

Frag Prime RG Depletion Mix

Blue

1 x 96 μl

1 x 384 μl

1st Strand Enzyme Mix

Brown

1 x 96 μl

1 x 384 μl

2nd Strand Buffer

Green

1 x 480 μl

2 x 960 μl

2nd Strand Enzyme Mix

Green

1 x 240 μl

1 x 960 μl

Rapid Ligation Buffer (5X)

Orange

1 x 480 μl

2 x 960 μl

T4 DNA Ligase

Orange

1 x 240 μl

1 x 960 μl

HiFi Plus Master Mix (2X)

White

1 x 600 μl

2 x 1.2 ml

Primer Mix

White

1 x 36 μl

1 x 144 μl

UDI Dilution Buffer

Clear

1 x 1.44 ml

1 x 5.76 ml

Performance Data

Resources

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

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