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PerfeCTa SYBR® Green SuperMix

Sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry
Features & Benefits
  • 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
  • Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
  • Supports efficient vortex mixing with proprietary anti-foaming formulation
  • SuperMix version provides maximum dye concentration for robust optical signal with small amplicons (i.e. microRNAtemplated cDNA)

 

PerfeCTa SYBR Green SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Product
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PerfeCTa SYBR Green SuperMix for iQ

Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  500 x 50 μL rxns (10 x 1.25 mL)
Part Number:  95053-500
Price:  $775.00
Add to Cart
Kit Size:  2000 x 50 μL rxns (1 x 50 mL)
Part Number:  95053-02K
Price:  $2,799.00
Add to Cart
Product
Kit Size
Order Info
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Quantity

PerfeCTa SYBR Green SuperMix

Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  500 x 50 μL rxns (10 x 1.25 mL)
Part Number:  95054-500
Price:  $775.00
Add to Cart
Kit Size:  2000 x 50 μL rxns (1 x 50 mL)
Part Number:  95054-02K
Price:  $2,799.00
Add to Cart
Product
Kit Size
Order Info
Price
Quantity

PerfeCTa SYBR Green SuperMix ROX

Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  500 x 50 μL rxns (10 x 1.25 mL)
Part Number:  95055-500
Price:  $775.00
Add to Cart
Kit Size:  2000 x 50 μL rxns (1 x 50 mL)
Part Number:  95055-02K
Price:  $2,799.00
Add to Cart
Product
Kit Size
Order Info
Price
Quantity

PerfeCTa SYBR Green SuperMix Low ROX

Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  500 x 50 μL rxns (10 x 1.25 mL)
Part Number:  95056-500
Price:  $775.00
Add to Cart
Kit Size:  2000 x 50 μL rxns (1 x 50 mL)
Part Number:  95056-02K
Price:  $2,799.00
Add to Cart

Description

PerfeCTa SYBR® Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStart™II antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.

What Customers Say

PerfeCTa SYBR Green SuperMix has been my first and successful experience that boosted my confidence for qPCR. I am really satisfied with its performance.”

Ohio University
Teaching Assistant
Details

Details

Contents

Single-tube, 2X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
  • AccuStart II Taq DNA Polymerase
  • SYBR Green I dye
  • Proprietary enzyme stabilizers and performance-enhancing additives.
  • Titrated reference dye (if applicable).
Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
Customer Testimonials

Customer Testimonials

PerfeCTa SYBR Green SuperMix

"PerfeCTa SYBR Green SuperMix has been my first and successful experience that boosted my confidence for qPCR. I am really satisfied with its performance."

Teaching Assistant | Ohio University

Details

Contents

Single-tube, 2X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.
  • AccuStart II Taq DNA Polymerase
  • SYBR Green I dye
  • Proprietary enzyme stabilizers and performance-enhancing additives.
  • Titrated reference dye (if applicable).

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Performance Data

Resources

Flyers

Product Manuals

Application Notes

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

FAQs

What is the amount of cDNA used per TaqMan or SYBR qPCR Assay?
Suggested input quantities of template are: cDNA corresponding to 1 pg to 100 ng of total RNA; 100 pg to 100 ng genomic DNA. For more information, please consult the PerfeCta qPCR Supermixes or PerfeCta SYBR Green Supermixes protocols.
Is there a SYBR® Green Fast Master Mix currently available
Yes. PerfeCTa SYBR Green Supermix is available. For further information, please consult the Kit protocol or product information.

Publications

Customer Testimonials

PerfeCTa SYBR Green SuperMix

"PerfeCTa SYBR Green SuperMix has been my first and successful experience that boosted my confidence for qPCR. I am really satisfied with its performance."

Teaching Assistant | Ohio University

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  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
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  • BioRad iCycler iQ™
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  • BioRad iQ™5

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