AccuStart II GelTrack® PCR SuperMix
Features & Benefits
- Contains premixed loading dye to streamline gel electrophoresis
- User-friendly 2X concentrated master mix simplifies reaction setup with exceptional room-temperature stability (≥30 days at 22°C) and withstands to repetitive freeze-thaw (≥ 20X)
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
- Stringent, ultrapure antibody hotstart ensures sensitive and precise assay performance
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AccuStart II GelTrack PCR SuperMix
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Description
AccuStart II GelTrack® PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. This kit improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart II Taq DNA polymerase which contains ultra-pure monoclonal antibodies that arrest polymerase prior to the initial PCR denaturation step. Hot start antibodies are rapidly and irreversibly denatured (1 minute at 94ºC), releasing a high yielding Taq DNA polymerase mutant with absolute maximum processivity and velocity from the first cycle. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
GelTrack Loading Dye is a mixture of blue and yellow electrophoresis-tracking dyes that migrate at approximately 4kb and 50 bp, and comes pre-mixed with the PCR reagents.
Details
Details
Contents
Storage & Handling
2X reaction buffer containing
- MgCl2 (3 mM)
- dATP, dCTP, dGTP, dTTP (0.4 mM each)
- AccuStart II Taq DNA Polymerase
- GelTrack loading dye and stabilizers.
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
Repeated freezing and thawing does not impair product performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Customer Testimonials
Customer Testimonials
AccuStart II GelTrack PCR SuperMix
"First-time grad students were running PCR with ease immediately. Efficiency was higher than previous reagents I have used."
Assistant Professor | Colorado State University
AccuStart II GelTrack PCR SuperMix
"Really loved how easy it was to use the product."
Ph.D. Student | University of Georgia
AccuStart II GelTrack PCR SuperMix
"Great performance at reduced price, and dye included is great for ease of use"
Fellow | The Scripps Research Institute
Details
Contents
Storage & Handling
2X reaction buffer containing
- MgCl2 (3 mM)
- dATP, dCTP, dGTP, dTTP (0.4 mM each)
- AccuStart II Taq DNA Polymerase
- GelTrack loading dye and stabilizers.
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
Repeated freezing and thawing does not impair product performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Resources
Product Manuals
CofA (PSFs)
- PSF-95136-04K-LOT-66233996.pdf
- PSF-95136-500-LOT-66233887.pdf
- PSF-95136-500-LOT-66231414.pdf
- PSF-95136-04K-LOT-66230758.pdf
- PSF-95136-500-LOT-66228936.pdf
- PSF-95136-04K-LOT-66226410.pdf
- PSF-95136-500-LOT-66222750.pdf
- PSF-95136-500-LOT-66221218.pdf
- PSF-95136-500-LOT-66216673.pdf
- PSF-95136-100-LOT-66215182.pdf
- PSF-95136-04K-LOT-66214121.pdf
- PSF-95136-500-LOT-66212210.pdf
- PSF-95136-500-LOT-66212213.pdf
- PSF-95136-500-LOT-66210059.pdf
- PSF-95136-100-LOT-66210283.pdf
- PSF-95136-04K-LOT-66208840.pdf
- PSF-95136-500-LOT-66206342.pdf
- PSF-95136-500-LOT-66202279.pdf
- PSF-95136-500-LOT-66201159.pdf
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- PSF-95136-500-LOT66140246.pdf
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- PSF-95136-100-Lot029539.pdf
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- PSF-95136-500-Lot028461.pdf
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- PSF-95136-500-Lot028175.pdf
- PSF-95136-04K-Lot027744.pdf
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- PSF-95136-500-Lot027601.pdf
- PSF-95136-500-Lot027065.pdf
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- PSF-95136-500-Lot025282.pdf
- PSF-95136-04K-Lot025280.pdf
- PSF-95136-500-Lot024747.pdf
- PSF-95136-500-Lot024746.pdf
- PSF-95136-500-Lot023235.pdf
- PSF-95136-500-Lot022446.pdf
- PSF-95136-500-Lot022445.pdf
- PSF-95136-500-Lot021880.pdf
- PSF-95136-04K-Lot023288.pdf
- PSF-95136-04K-Lot022349.pdf
- PSF-95136-500-Lot021003.pdf
- PSF-95136-100-Lot014838.pdf
- PSF-95136-100-Lot66177802.pdf
- PSF-95136-500-Lot66176994.pdf
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- PSF-95136-04K-Lot2366158890.pdf
- PSF-95136-04K-Lot66162065.pdf
- PSF-95136-04K-Lot66170565.pdf
- PSF-95136-500-Lot66160792.pdf
- PSF-95136-500-Lot66170855.pdf
Click here to see all CofA (PSFs)
SDSs
Publications
SYPL1 defines a vesicular pathway essential for sperm cytoplasmic droplet formation and male fertility
Jiali Liu - 2023
AbstractThe cytoplasmic droplet is a conserved dilated area of cytoplasm situated at the neck of the sperm flagellum. Viewed as residual cytoplasm inherited from late spermatids, the cytoplasmic droplet contains numerous saccular elements as its key content. However, the origin of these saccules and the function of the cytoplasmic droplet have long been speculative. Here, we identify the molecular origin of these cytoplasmic droplet components by uncovering a vesicle pathway essential for formation and sequestration of saccules within the cytoplasmic droplet. This process is governed by a transmembrane protein SYPL1 and its interaction with VAMP3. Genetic ablation of SYPL1 in mice reveals that SYPL1 dictates the formation and accumulation of saccular elements in the forming cytoplasmic droplet. Derived from the Golgi, SYPL1 vesicles are critical for segregation of key metabolic enzymes within the forming cytoplasmic droplet of late spermatids and epididymal sperm, which are required for sperm development and male fertility. Our results uncover a mechanism to actively form and segregate saccules within the cytoplasmic droplet to promote sperm fertility.
Inhibition of serum- and glucocorticoid-induced kinase 1 ameliorates hydrocephalus in preclinical models
Alexandra Hochstetler - 2023
AbstractBackground
Hydrocephalus is a pathological accumulation of cerebrospinal fluid (CSF), leading to ventriculomegaly. Hydrocephalus may be primary or secondary to traumatic brain injury, infection, or intracranial hemorrhage. Regardless of cause, current treatment involves surgery to drain the excess CSF. Importantly, there are no long-term, effective pharmaceutical treatments and this represents a clinically unmet need. Many forms of hydrocephalus involve dysregulation in water and electrolyte homeostasis, making this an attractive, druggable target.
Methods
In vitro, a combination of electrophysiological and fluid flux assays was used to elucidate secretory transepithelial electrolyte and fluid flux in a human cell culture model of the choroid plexus epithelium and to determine the involvement of serum-, glucocorticoid-induced kinase 1 (SGK1). In vivo, MRI studies were performed in a genetic rat model of hydrocephalus to determine effects of inhibition of SGK1 with a novel inhibitor, SI113.
Results
In the cultured cell line, SI113 reduced secretory transepithelial electrolyte and fluid flux. In vivo, SI113 blocks the development of hydrocephalus with no effect on ventricular size of wild-type animals and no overt toxic effects. Mechanistically, the development of hydrocephalus in the rat model involves an increase in activated, phosphorylated SGK1 with no change in the total amount of SGK1. SI113 inhibits phosphorylation with no changes in total SGK1 levels in the choroid plexus epithelium.
Conclusion
These data provide a strong preclinical basis for the use of SGK1 inhibitors in the treatment of hydrocephalus.
Non-breeding season records of warblers in the Phylloscopus reguloides lineage from Thailand and Myanmar
Round, Philip D. - 2023
AbstractA total of 20 individual Phylloscopus warblers in the ‘Blyth’s Leaf
Warbler P. reguloides lineage’, caught for ringing in the non-breeding season in
Thailand and Myanmar, were resolved via mtDNA assay as P. reguloides (seven
individuals), Claudia’s Leaf Warbler P. claudiae (12) and Hartert’s Leaf Warbler P.
goodsoni (one). As expected, P. claudiae proved to be the most widely distributed.
The occurrence of four P. claudiae on an island in the Thai Gulf, alongside large
numbers of typically Sundaic wintering species on northbound migration during
late March‒early April, indicates that its non-breeding season range probably
extends further south than previously recognised, into that subregion.
The effect of a mass distribution of insecticide-treated nets on insecticide resistance and entomological inoculation rates of Anopheles gambiae s.l. in Bandundu City, Democratic Repub`lic of Congo
Emery Metelo-Matubi - 2021
AbstractIntroduction
insecticide-treated nets (ITNs) remain the mainstay of malaria vector control in the Democratic Republic of Congo. However, insecticide resistance of malaria vectors threatens their effectiveness. Entomological inoculation rates and insecticide susceptibility in Anopheles gambiae s.l. were evaluated before and after mass distribution of ITNs in Bandundu City for possible occurrence of resistance.
Methods
a cross-sectional study was conducted from 15th July 2015 to 15th June 2016. Adult mosquitoes were collected using pyrethrum spray catches and human landing catches and identified to species level and tested for the presence of sporozoites. Bioassays were carried out before and after distribution of ITNs to assess the susceptibility of adult mosquitoes to insecticides. Synergist bioassays were also conducted and target site mutations assessed using Polymerase chain reaction (PCR).
Results
a total of 1754 female An. gambiae s.l. were collected before and after deployment of ITNs. Fewer mosquitoes were collected after the distribution of ITNs. However, there was no significant difference in sporozoite rates or the overall entomological inoculation rate before and after the distribution of ITNs. Test-mosquitoes were resistant to deltamethrin, permethrin, and Dichlorodiphenyltrichloroethane but susceptible to bendiocarb. Pre-exposure of mosquitoes to Piperonyl butoxide increased their mortality after exposure to permethrin and deltamethrin. The frequency of the Kinase insert domain receptor (kdr)-West gene increased from 92 to 99% before and after the distribution of nets, respectively.
Conclusion
seasonal impacts could be a limiting factor in the analysis of these data; however, the lack of decrease in transmission after the distribution of new nets could be explained by the high-level of resistance to pyrethroid.
SYK Inhibition Potentiates the Effect of Chemotherapeutic Drugs on Neuroblastoma Cells In Vitro
Conny Tummler - 2019
AbstractNeuroblastoma is a malignancy arising from the developing sympathetic nervous system and the most common and deadly cancer of infancy. New therapies are needed to improve the prognosis for high-risk patients and to reduce toxicity and late effects. Spleen tyrosine kinase (SYK) has previously been identified as a promising drug target in various inflammatory diseases and cancers but has so far not been extensively studied as a potential therapeutic target in neuroblastoma. In this study, we observed elevated SYK gene expression in neuroblastoma compared to neural crest and benign neurofibroma. While SYK protein was detected in the majority of examined neuroblastoma tissues it was less frequently observed in neuroblastoma cell lines. Depletion of SYK by siRNA and the use of small molecule SYK inhibitors significantly reduced the cell viability of neuroblastoma cell lines expressing SYK protein. Moreover, SYK inhibition decreased ERK1/2 and Akt phosphorylation. The SYK inhibitor BAY 61-3606 enhanced the effect of different chemotherapeutic drugs. Transient expression of a constitutive active SYK variant increased the viability of neuroblastoma cells independent of endogenous SYK levels. Collectively, our findings suggest that targeting SYK in combination with conventional chemotherapy should be further evaluated as a treatment option in neuroblastoma.
Customer Testimonials
AccuStart II GelTrack PCR SuperMix
"First-time grad students were running PCR with ease immediately. Efficiency was higher than previous reagents I have used."
Assistant Professor | Colorado State University
AccuStart II GelTrack PCR SuperMix
"Really loved how easy it was to use the product."
Ph.D. Student | University of Georgia
AccuStart II GelTrack PCR SuperMix
"Great performance at reduced price, and dye included is great for ease of use"
Fellow | The Scripps Research Institute