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sparQ DNA Library Prep Kit

Streamlined, versatile single-tube solution for high quality library prep
Features & Benefits
  • Fast, easy single-tube solution completes library prep in 2.5 hours
  • Suitable for a wide range of input amounts from as low as 250 pg
  • Optimized chemistry ensuring superior library prep sensitivity and efficiency
  • Higher library yields compared to other library prep kits
  • High efficiency enables PCR-free workflow from 100 ng input

 

sparQ DNA Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Product
Kit Size
Order Info
Price
Quantity

sparQ DNA Library Prep Kit

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Kit Size:
Part Number:
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Quantity:
Kit Size:  24 rxns
Part Number:  95191-024
Price:  $216.00
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Kit Size:  96 rxns
Part Number:  95191-096
Price:  $864.00
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Description

sparQ DNA Library Prep Kit is optimized for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms. The simplified protocol speeds up library prep to 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 250 pg to 1 ug. DNA polishing reactions are streamlined into a single step to convert fragmented DNA into 5'-phosphorylated and 3'-dA-tailed DNA fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.


 

NGS Automation

Quantabio sparQ Frag & Library Prep Kit can be fully automated

Currently sparQ Frag & Library Prep Kit is supported by the following vendors:

Vendor Automation Platform(s)
PerkinElmer Applied Genomics SCICLONE G3 NGSx
Tecan DreamPrep™ NGS
Beckman Coulter in development

 

Looking for more information or for different automation vendor?

Contact Us

 


 

sparQ UDI Adapter Resources

Setting up sparQ Library Prep kits in Illumina Experiment Manager

Video Tutorial

Watch the step-by-step tutorial illustrating the sparQ DNA Library Prep Kit protocol


 

sparQ UDI Adapter Resource

For help setting up UDI’s in Illumina Experiment Manager

Download


 

Featured Video

sparQ NGS Library Prep Solutions


 

Details

Details

Contents

 

 

Volume

Component Description

Cap Color

95191-024

95191-096

DNA Polishing Enzyme Mix

Blue

1 x 0.24 ml

1 x 0.96 ml

DNA Polishing Buffer

Blue

1 x 0.12 ml

1 x 0.48 ml

DNA Ligase

Orange

1 x 0.24 ml

1 x 0.96 ml

DNA Rapid Ligation Buffer

Orange

1 x 0.48 ml

2 x 0.96 ml

HiFi PCR Master Mix (2X)

White

1 x 0.60 ml

2 x 1.25 ml

Primer Mix

White

1 x 0.072 ml

1 x 0.144 ml

Customer Testimonials

Customer Testimonials

sparQ DNA Library Prep Kit

"Very easy to use."

Scientist | University Sherbrooke

Details

Contents

 

 

Volume

Component Description

Cap Color

95191-024

95191-096

DNA Polishing Enzyme Mix

Blue

1 x 0.24 ml

1 x 0.96 ml

DNA Polishing Buffer

Blue

1 x 0.12 ml

1 x 0.48 ml

DNA Ligase

Orange

1 x 0.24 ml

1 x 0.96 ml

DNA Rapid Ligation Buffer

Orange

1 x 0.48 ml

2 x 0.96 ml

HiFi PCR Master Mix (2X)

White

1 x 0.60 ml

2 x 1.25 ml

Primer Mix

White

1 x 0.072 ml

1 x 0.144 ml

Performance Data

Resources

Flyers

Product Manuals

Application Notes

Brochures

CofA (PSFs)

SDSs

Publications

Customer Testimonials

sparQ DNA Library Prep Kit

"Very easy to use."

Scientist | University Sherbrooke

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