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Pathogen Detection

Quantabio Pathogen Detection

Quantabio reagents are the gold standard for sensitive and reliable quantitative assay performance in PCR, qPCR and NGS. Our proprietary ToughMix® reagents enable efficient amplification for challenging PCR inhibitors.

These reagents are used globally in thousands of clinical-translational and hundreds of public health laboratories to help identify and detect pathogens in a variety of infectious disease assays such as:

  • Influenza
  • COVID-19
  • West Nile
  • Zika
  • Polio
Workflow Solutions

Dried blood spots

Clinical/ Environmental sample

DNA Extraction

DNA or RNA Extraction

Amplification

Amplification
Detection
Detection
Customer Testimonials
PerfeCTa MultiPlex qPCR ToughMix

"The Perfecta Multiplex qPCR ToughMix greatly simplified my qPCR assay by removing DNA isolation steps and complexity of passive reference dyes. I will definitely be using this product in the future."

Bethany B. | Biotech company
PerfeCTa qPCR ToughMix

"This eliminates a costly and time consuming step to remove inhibitors manually, so it has saved us money on reagents and time for our small lab."

Max O. | Conservationist
PerfeCTa qPCR ToughMix

"Higher sensitivity, positives at a lower Cq. Great results with plant DNA with high inhibition level."

Paula S. | Lab Coordinator INIAV
UltraPlex 1-Step ToughMix

"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."

Rhoel R. D., PhD, MPH Professor of Infectious Diseases UF Eminent Scholar Director | CDC Southeastern Regional Center of Excellence for Vector Borne Diseases
qScript 1-Step Virus ToughMix

"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."

Guillermo C. | Project Coordinator Sick Kids Molecular Diagnostics Lab
qScript XLT 1-Step RT-qPCR ToughMix

"It is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."

Olivia L. | Senior Research Engineer

Compare Products

Determine which RNA master mix solution is right for you.

 

qScript XLT 1-Step RT-qPCR ToughMix qScript 1-Step Virus ToughMix UltraPlex 1-Step ToughMix
Recommended inCDC protocol x x
Detection purified RNA x x x
Extraction-free detection x x
xSaliva-direct detection x
Formulation 2X master mix 2X master mix 4X master mix
Taq Hot Start Yes Yes Yes
RT Hot Start No Yes Yes
Chemistry 1 tube SuperMix 1 tube SuperMix 1 tube SuperMix
Performance ++++ ++++ ++++
Cycling Conditions Fast or Standard qPCR Cycling Fast or Standard qPCR Cycling Fast or Standard qPCR Cycling
Multiplex Capabilty Up to 4 targets Up to 4 targets Up to 5 targets
Sensitivity +++ ++++ ++++
Inhibitor Tolerance Yes Yes Yes

 

Determine which DNA master mix solution is right for you.

 

PerfeCTa qPCR ToughMix PerfeCTa Multiplex qPCR ToughMix
Features
Concentration 2x 5x
Sample Type gDNA, cDNA gDNA, cDNA
Fast Cycling Yes Yes
Multiplex Compatibility Up to 2 targets Up to 2 targets
Carryover Contamination Control N/A N/A
Applications
Gene Expression x x
MicroRNA
ChIP
SNP Genotyping
Inhibitor Tolerant ** x
High Yield x **
Available Formats
Master Mix x x
Packaging (Rxns/Units) 250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1000 Rxns
5000 Rxns

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

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