Quantabio Pathogen Detection
Quantabio reagents are the gold standard for sensitive and reliable quantitative assay performance in PCR, qPCR and NGS. Our proprietary ToughMix® reagents enable efficient amplification for challenging PCR inhibitors.
These reagents are used globally in thousands of clinical-translational and hundreds of public health laboratories to help identify and detect pathogens in a variety of infectious disease assays such as:
- West Nile
"The Perfecta Multiplex qPCR ToughMix greatly simplified my qPCR assay by removing DNA isolation steps and complexity of passive reference dyes. I will definitely be using this product in the future."
"This eliminates a costly and time consuming step to remove inhibitors manually, so it has saved us money on reagents and time for our small lab."
"Higher sensitivity, positives at a lower Cq. Great results with plant DNA with high inhibition level."
"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."
"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."
"It is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."
Determine which RNA master mix solution is right for you.
|qScript XLT 1-Step RT-qPCR ToughMix||qScript 1-Step Virus ToughMix||UltraPlex 1-Step ToughMix|
|Recommended inCDC protocol||x||x|
|Detection purified RNA||x||x||x|
|Formulation||2X master mix||2X master mix||4X master mix|
|Taq Hot Start||Yes||Yes||Yes|
|RT Hot Start||No||Yes||Yes|
|Chemistry||1 tube SuperMix||1 tube SuperMix||1 tube SuperMix|
|Cycling Conditions||Fast or Standard qPCR Cycling||Fast or Standard qPCR Cycling||Fast or Standard qPCR Cycling|
|Multiplex Capabilty||Up to 4 targets||Up to 4 targets||Up to 5 targets|
Determine which DNA master mix solution is right for you.
|PerfeCTa qPCR ToughMix||PerfeCTa Multiplex qPCR ToughMix|
|Sample Type||gDNA, cDNA||gDNA, cDNA|
|Multiplex Compatibility||Up to 2 targets||Up to 2 targets|
|Carryover Contamination Control||N/A||N/A|
|Packaging (Rxns/Units)||250 Rxns