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qScript 1-Step Virus ToughMix

Superior sensitivity for viral RNA detection
Features & Benefits
  • Superior Sensitivity – higher yields optimized for lower viral RNA inputs
  • Tough Tested – tolerant to a wide range of PCR inhibitors
  • Multiplexing – Supports highly sensitive detection for up to four targets
  • Broad Dynamic Range – Reliable data from your precious samples every time

 

qScript 1-Step Virus ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Order Info
Product
Kit Size
Order Info
qScript 1-Step Virus ToughMix
Request Sample
Kit Size:
Order Info:
95131-100 x 20 μL rxns (1 x 1 mL)
95131-500 x 20 μL rxns (5 x 1 mL)
95131-2000 x 20 μL rxns (1 x 20 mL)
Product
Kit Size
Order Info
qScript 1-Step Virus ToughMix Low ROX
Request Sample
Kit Size:
Order Info:
95212-2000 x 20 μL rxns (1 x 20 mL)
95212-100 x 20 μL rxns (1 x 1 mL)
95212-500 x 20 μL rxns (5 x 1 mL)

Description

qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labeled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats. qScript 1-Step Virus ToughMix contains all required components for RT-qPCR except RNA template and primer/probe assays.

qScript 1-Step Virus ToughMix is powered by an engineered reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures, that includes a RT Hot-Start technology to suppresses non-specific primer extension by the RT before cDNA synthesis. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. These features combined with a stringent mAb hot-start Taq polymerase and ToughMix reagent technology to neutralize many common PCR inhibitors, provide reproducible low-copy quantification as well as extended room temperature stability of fully assembled reactions for automated reaction assembly.

qScript 1-Step Virus ToughMix does not contain an internal reference dye.

Request Sample

 

What Customers Say
qScript 1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment

G. Casallo
Sick Kids – Molecular Diagnostics Lab
Details

Details

Contents
  • 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride,
  • qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,
  • AccuVue blue qPCR dye, and stabilizers
Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Details

Contents
  • 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride,
  • qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,
  • AccuVue blue qPCR dye, and stabilizers

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Performance Data

Resources

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products