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AccuStart Long Range SuperMix

Superior sensitivity and multiplexing for DNA amplification of long targets
Features & Benefits
  • Amplify +24 kb gDNA and +40 kb lambda DNA
  • 4x concentration enables superior sensitivity with low inputs (100 pg)
  • Multiplex up to 6 targets with each target up to 6 kb in length
  • Stabilized, single-tube SuperMix minimizes pipetting errors and hands-on-time
  • Reliable lot-to-lot reproducibility ensures consistent results
  • Amplification through uracils and primers containing inosines/uracils

AccuStart Long Range SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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AccuStart Long Range SuperMix
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Description

The AccuStart Long Range SuperMix is a 4x, ready-to-use solution that contains all the components for long range target amplifications, including a blend of two hot-start thermostable DNA polymerases (one with strong proof-reading activity) and an optimized buffer to ensure high efficiency, sensitivity and specificity.

The SuperMix enables routine and easy amplification of up to 24kb target from gDNA with high accuracy (> 10-fold better fidelity than Taq) and accommodates targets with broad GC-content (no separate GC buffer needed). This product is also capable of amplifying multiple targets simultaneously and is suitable for end point PCR, template prep for sanger sequencing, NGS, cloning and HLA typing.

Details

Details

Contents
  • 4x ready-to-use solution
Customer Testimonials

Customer Testimonials

AccuStart Long Range SuperMix

"The AccuStart Long Range SuperMix produced a clean long-range product (10 kbp) on our first try! We are excited to have a reliable and easy-to-use polymerase to produce DNA for single-molecule studies."

Danielle M. | Professor, Stanford University

Details

Contents
  • 4x ready-to-use solution

Resources

Publications

Genomic Characterization of the Fruity Aroma Gene, FaFAD1, Reveals a Gene Dosage Effect on γ-Decalactone Production in Strawberry (Fragaria × ananassa)
Youngjae Oh - 2021
Abstract
Strawberries produce numerous volatile compounds that contribute to the unique flavors of fruits. Among the many volatiles, γ-decalactone (γ-D) has the greatest contribution to the characteristic fruity aroma in strawberry fruit. The presence or absence of γ-D is controlled by a single locus, FaFAD1. However, this locus has not yet been systematically characterized in the octoploid strawberry genome. It has also been reported that the volatile content greatly varies among the strawberry varieties possessing FaFAD1, suggesting that another genetic factor could be responsible for the different levels of γ-D in fruit. In this study, we explored the genomic structure of FaFAD1 and determined the allele dosage of FaFAD1 that regulates variations of γ-D production in cultivated octoploid strawberry. The genome-wide association studies confirmed the major locus FaFAD1 that regulates the γ-D production in cultivated strawberry. With the hybrid capture-based next-generation sequencing analysis, a major presence–absence variation of FaFAD1 was discovered among γ-D producers and non-producers. To explore the genomic structure of FaFAD1 in the octoploid strawberry, three bacterial artificial chromosome (BAC) libraries were developed. A deletion of 8,262 bp was consistently found in the FaFAD1 region of γ-D non-producing varieties. With the newly developed InDel-based codominant marker genotyping, along with γ-D metabolite profiling data, we revealed the impact of gene dosage effect for the production of γ-D in the octoploid strawberry varieties. Altogether, this study provides systematic information of the prominent role of FaFAD1 presence and absence polymorphism in producing γ-D and proposes that both alleles of FaFAD1 are required to produce the highest content of fruity aroma in strawberry fruit.

Customer Testimonials

AccuStart Long Range SuperMix

"The AccuStart Long Range SuperMix produced a clean long-range product (10 kbp) on our first try! We are excited to have a reliable and easy-to-use polymerase to produce DNA for single-molecule studies."

Danielle M. | Professor, Stanford University

Product Finder

Select Your Assay

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Multiplexing (more than 3 targets)

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