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qScript Flex cDNA Synthesis Kit

Flexible first-strand cDNA synthesis system
Features & Benefits
  • Separate components for user-defined RT priming method, (oligo-dT, random hexamer, or GSP)
  • Highly sensitive first-strand cDNA synthesis of RNA sequences ≤ 12kb for quantitative and conventional two-step RT-PCR
  • Broad linear dynamic detection range with limiting (10pg) or plentiful (4ug) total RNA samples
  • Maximize cDNA yield with proprietary Priming Enhancer additive

 

qScript Flex cDNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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qScript Flex cDNA Synthesis Kit

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Kit Size:  25 x 20 μL rxns (1 x 25 µL)
Part Number:  95049-025
Price:  $158.00
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Kit Size:  100 x 20 μL rxns (1 x 100 µL)
Part Number:  95049-100
Price:  $511.00
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Description

The qScript Flex cDNA Synthesis Kit is configured in separate components for supporting multiple RT priming strategies. The kit provides optimized, flexible components for priming with oligo-dT, random hexamers, gene-specific primer (GSP), or any combination thereof. This unique 5X master mix of buffer, magnesium, stabilizers and dNTPs simplifies reaction assembly and ensures robust and reproducible synthesis of first strand product from 10 pg to 4µg of total RNA or purified polyA+ RNA template. The resulting cDNA product is directly compatible with current real-time RT-PCR methods or end-point RT-PCR. Length of cDNA product is dependent upon priming strategy and quality of the RNA template. Oligo dT or GSP can be used for two-step RT-PCR of RNA targets up to 12 kb. Random primer is suitable for RT-PCR of RNA targets less than 1 kb. Any of the three priming methods are suitable for real-time quantitative RT PCR (RT-qPCR).

For one-step RT-PCR of targets less than 5 kb, see our qScript XLT 1-Step RT-PCR Kit

Details

Details

Contents
  • Master mix, 5X concentrated solution of optimized reaction buffer, magnesium, dNTP blend, proprietary stabilizers and additives
  • Oligo dT(20), 10X concentrated with Priming Enhancer
  • Random Primer, 10X concentrated with Priming Enhancer
  • Priming Enhancer, 10X concentrated
  • Stabilized qScript Reverse Transcriptase with ribonuclease inhibitor protein (RIP)
  • Nuclease-free water

Details

Contents
  • Master mix, 5X concentrated solution of optimized reaction buffer, magnesium, dNTP blend, proprietary stabilizers and additives
  • Oligo dT(20), 10X concentrated with Priming Enhancer
  • Random Primer, 10X concentrated with Priming Enhancer
  • Priming Enhancer, 10X concentrated
  • Stabilized qScript Reverse Transcriptase with ribonuclease inhibitor protein (RIP)
  • Nuclease-free water

Performance Data

Resources

Product Manuals

Application Notes

CofA (PSFs)

SDSs

FAQs

What is the qScript™ Flex cDNA Kit? How is it different from the qScript™ cDNA Synthesis Kit?
The qScript Flex cDNA Synthesis Kit is an easy-to-use and highly efficient kit for the synthesis of first-strand cDNA that enables your choice of cDNA priming method. Various RT-PCR applications may require different priming strategies for optimal performance and this kit provides optimized reagents for priming with oligo(dT)20, random primer, gene-specific primer (GSP), or any combination thereof. Therefore, qScript™ Flex cDNA Kit is ideal for developing the best protocol for your application. The qScript™ Flex cDNA Kit also allows the use of Oligo dT or gene-specific primers (GSP) for long RT-PCR of RNA targets up to 12 kb. The qScript cDNA Synthesis Kit consists of an optimized blend of random and oligo(dT) primers. It provides robust, consistent and unbiased first-strand synthesis over a broad range of RNA template concentrations. qScript™ cDNA Synthesis Kit is well suited for short RT-PCR apllications (i.e. qRT-PCR) and is not recommended for amplification of RNA's longer than 1kb. When using a mixture of the Oligo dT and Random Primer solutions, the qScript™ Flex cDNA Kit achieves first-strand synthesis over a broad range of RNA template concentrations and its performance is comparable to that of the qScript™ cDNA Synthesis Kit. With both kits, the resulting cDNA product is directly compatible with current real-time RT-PCR methods or end-point RT-PCR.
What is the Flex Kit? How is it different from the cDNA synthesis Kit? Can we use either an oligo dT or a random priming method?
The Flex Kit supports the use of multiple priming strategies. When using a combination of the oligo dT primer solution and random primer solution, the linear dynamic range and qRT-PCR performance is comparable to that of the qScript cDNA Synthesis Kit. In addition to retaining high performance with limiting amounts of RNA, the Flex kit was also optimized to provide long cDNA synthesis with oligo dT primer. So its composition is different from that of the cDNA synthesis Kit. When using oligo dT primer alone, it is unquestionably one of the easiest to use and most sensitive cDNA kits on the market

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