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qScript XLT 1-Step RT-PCR Kit

Tough-tested One-Step Reverse Transcriptase PCR (RT-PCR) in a simplified, 2-component reagent system.
Features & Benefits
  • ToughMix reagent technology withstands PCR inhibitors commonly found in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
  • Optional GelTrack dye streamlines workflow for gel electrophoresis
  • Temperature stabilized for support reaction assembly at convenient ambient room temperatures
  • Preblended with ribonuclease inhibitor protein to preserve RNA integrity during incubation
  • 3′-exonuclease proof-reading polymerase supports high-fidelity downstream applications

qScript XLT 1-Step RT-PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Order Info
Product
Kit Size
Order Info
qScript XLT 1-Step RT-PCR Kit
Request Sample
Kit Size:
Order Info:
20 x 25 μL rxns
200 x 25 μL rxns

Description

The qScript XLT One-Step RT-PCR Kit is a convenient and highly sensitive 2-reagent system for amplification of complex RNA templates exceeding 4 kb in length. Both enzyme incubation sequences are carried out in the same reaction mixture without opening between procedures. Advanced qScript XLT reverse transcriptase mutant possesses elevated temperature stability and improved template binding affinity for large complex RNA templates. Supports a wide-range of challenging starting materials with PCR-inhibitor neutralizing ToughMix additives and delivers consistent, reliable assay performance. Ultrapure AccuStart II hot-start Taq DNA polymerase with 3'-exonuclease proof-reading activity provides stringent activation control for sensitive and precise target amplification.
Details

Details

Contents
  • 25X concentrated One-Step RT master mix containing: qScript XLT reverse transcriptase ribonuclease inhibitor protein (RIP).
  • 2X concentrated One-Step HiFi PCR ToughMix containing:
    • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
    • AccuStart II Taq DNA Polymerase with added 3′-exonuclease proofreading activity
    • Proprietary enzyme stabilizers and performance-enhancing additives.
    • Titrated reference dye (if applicable).
  • GelTrack® Loading Dye: 50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoretic mobility dyes. (Does not contain density reagent)
  • Nuclease-free water

Details

Contents
  • 25X concentrated One-Step RT master mix containing: qScript XLT reverse transcriptase ribonuclease inhibitor protein (RIP).
  • 2X concentrated One-Step HiFi PCR ToughMix containing:
    • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
    • AccuStart II Taq DNA Polymerase with added 3′-exonuclease proofreading activity
    • Proprietary enzyme stabilizers and performance-enhancing additives.
    • Titrated reference dye (if applicable).
  • GelTrack® Loading Dye: 50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoretic mobility dyes. (Does not contain density reagent)
  • Nuclease-free water

Resources

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

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