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PCR
- Real-Time Quantitative PCR
  - SYBR Green Detection
    - DNA
  - Probe-based Detection
  - RNA
- Conventional PCR
  - DNA
  - RNA
Next Generation Sequencing (NGS)
- DNA
- RNA
Reverse Transcription
- First-Strand cDNA Synthesis
Sample Preparation
- DNA
- RNA
Real-Time qPCR
Q qPCR Instrument

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Application Notes

PCR
- Real-Time Quantitative PCR
- Conventional PCR
  - DNA
    repliQa HiFi ToughMix: Best Practices for Optimal PCR Amplification
    Fast and Efficient cDNA Synthesis and PCR Amplification for Molecular Cloning
    Improving PCR amplification of different NGS methods using repliQa HiFi ToughMix
    Optimal PCR amplification for GC-rich templates using repliQa HiFi ToughMix® and enhancers
    Rapid 16S Metagenomic Library Preparation for Oxford Nanopore Technologies (ONT)^® Platform
    Rapid library preparation for 16S rRNA sequencing
  - RNA
    RT-qPCR Optimization Guide
Next Generation Sequencing (NGS)
- DNA
  High-throughput library preparation for whole genome and target panel-based sequencing
  Quality Control for PCR-Free WGS Libraries: A Guideline for TapeStation® Analysis of PCR-Free Library Preparation with Y-Shaped Adapters
  Fast and Efficient cDNA Synthesis and PCR Amplification for Molecular Cloning
  Increased control over longer DNA fragmentation patterns using lower temperature with sparQ DNA Frag & Library Prep Kit
  Streamlined and high accuracy whole genome and transcription sequencing using Singular Genomics G4TM platform and Quantabio sparQ library prep kits
  Size Selection sparQ PureMag Beads
  Rapid 16S Metagenomic Library Preparation for Oxford Nanopore Technologies (ONT)^® Platform
  sparQ UDI Adapter sequence overview
  Rapid library preparation for 16S rRNA sequencing
  RNA cleanup and size selection using sparQ PureMag Beads
  sparQ PureMag Beads Room Temperature Stability
  Optimization Guide
  How to Set Up Illumina Experiment Manager Using sparQ Adapter Barcode Sets
  Single-tube solutions to streamline DNA library preparation and improve sequencing results while lowering costs
  Simple, high quality, and cost-effective library preparation solutions for NGS
  Companion sparQ Universal Library Quant Kit
  Automation of the sparQ DNA Library Prep Kit on the PerkinElmer® Sciclone G3
- RNA
  Streamlined and high accuracy whole genome and transcription sequencing using Singular Genomics G4TM platform and Quantabio sparQ library prep kits
  Improved RNA-Seq Library Prep: Further simplifying whole transcriptome library prep using modifications to the sparQ RNA-Seq HMR Kit
  Simplified RNA-Seq Library Prep: Improved RNA sequencing results for FFPE and blood samples
  Streamlined Whole Transcriptome sequencing using Singular Genomics G4 platform and Quantabio sparQ RNA-Seq HMR Kit
  Rapid 16S Metagenomic Library Preparation for Oxford Nanopore Technologies (ONT)^® Platform
  sparQ UDI Adapter sequence overview
Reverse Transcription
- First-Strand cDNA Synthesis
  Fast and Efficient cDNA Synthesis and PCR Amplification for Molecular Cloning
  qScript cDNA Synthesis Selection Guide
Sample Preparation
- DNA
  Extracta Product Selection Guide
  Mei Baker - A Multiplex Real-time PCR Assay to Detect SCID and SMN1
- RNA
  Extracta Product Selection Guide
Real-Time qPCR
RT-qPCR Optimization Guide
Extraction-free COVID-19 detection with Quantabio’s RT-qPCR ToughMixes
High Capacity COVID-19 testing
Quantabio in Public Health
Q qPCR Instrument
Guidelines for the Detection of Bacterial DNA with Coriolis
Rapid and accurate quantification of Illumina NGS libraries using sparQ Fast Library Quant Kit on the Q real-time qPCR instrument
Quick Guide to Analysis of sparQ Fast Library Quantification Data
Q qPCR Cycler Dye Detection
Computer Recommendations for Q
Plugging in Q
Rapid and Accurate Quantification of Illumina NGS Libraries Using the Q Real-Time qPCR Instrument
Remove Tubes from Last Run
Q in the Field - Alternative Power Sources
Q qPCR Cycler & PerfeCTa SYBR Green FastMix
Q 96 Well Plate Compatibility
Q-qPCR v1.0.1 release notes

Miscellaneous
Improved applications for RNA analysis
sparQ mRNA-Seq: Consistent and high-quality mRNA library preparation from both abundant and limiting samples
PCR Primer Design Guide
Q-qPCR v1.0.1 release notes

Publications

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Select Your Assay

qPCR & cDNA Synthesis

Conventional PCR (Endpoint)

Genotyping

Next Generation Sequencing

Sample Prep

Cloning

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Starting Template

DNA

Dried blood spots (DBS)

Mammalian tissues

RNA

RNA (1-step RT-PCR)

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Assay Format

1 step RT-qPCR (excellent for applications such as pathogen detection)

2 Step RT-qPCR (excellent for applications such as gene expression)

cDNA synthesis only

qPCR

Endpoint PCR

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Detection Chemistry

Dye (SYBR Green)

Molecular Probe (TaqMan)

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Multiplexing (more than 3 targets)

Yes

No

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Is gene-specific priming (GSP) required?

Yes

No

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What current Reverse Transcriptase or cDNA kit are you using?

ABI High Capacity Kit

Biorad iScript RT Supermix

Biorad iScript cDNA Synthesis Kit

Superscript II

Superscript III

Superscript VILO

Other

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Select the group which contains your real-time PCR cycler

Compatible with Low ROX kits

Applied Biosystems 7500
Applied Biosystems 7500 Fast
Stratagene Mx3000P®
Stratagene Mx3005P™
Stratagene Mx4000™
Applied Biosystems ViiA 7
Applied Biosystems QuantStudio™
Agilent AriaMx
Douglas Scientific IntelliQube®

Compatible with ROX kits

Applied Biosystems 5700
Applied Biosystems 7000
Applied Biosystems 7300
Applied Biosystems 7700
Applied Biosystems 7900
Applied Biosystems 7900HT
Applied Biosystems 7900 HT Fast
Applied Biosystems StepOne™
Applied Biosystems StepOnePlus™

Compatible with standard kits

Quantabio Q
BioRad CFX
Roche LightCycler 480
QIAGEN Rotor-Gene Q
Azure Cielo 6
Azure Cielo 3
Analytik Jena qTower
Analytik Jena qTOWERiris
Other

Compatible with Bio-Rad iCycler iQ systems

BioRad iCycler iQ™
BioRad MyiQ™
BioRad iQ™5

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Choose your application from the categories below

Fragmentation & Library Preparation

Library Preparation

Library Amplification

Library Quantification

Purification & Size Selection

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Products

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