US
array(52) {
  ["SERVER_SOFTWARE"]=>
  string(6) "Apache"
  ["REQUEST_URI"]=>
  string(89) "/product-category/products/pcr/real-time-quantitative-pcr/rna-real-time-quantitative-pcr/"
  ["PATH"]=>
  string(49) "/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin"
  ["PP_CUSTOM_PHP_INI"]=>
  string(48) "/var/www/vhosts/system/quantabio.com/etc/php.ini"
  ["PP_CUSTOM_PHP_CGI_INDEX"]=>
  string(19) "plesk-php74-fastcgi"
  ["SCRIPT_NAME"]=>
  string(10) "/index.php"
  ["QUERY_STRING"]=>
  string(0) ""
  ["REQUEST_METHOD"]=>
  string(3) "GET"
  ["SERVER_PROTOCOL"]=>
  string(8) "HTTP/1.1"
  ["GATEWAY_INTERFACE"]=>
  string(7) "CGI/1.1"
  ["REDIRECT_URL"]=>
  string(89) "/product-category/products/pcr/real-time-quantitative-pcr/rna-real-time-quantitative-pcr/"
  ["REMOTE_PORT"]=>
  string(5) "46792"
  ["SCRIPT_FILENAME"]=>
  string(48) "/var/www/vhosts/quantabio.com/httpdocs/index.php"
  ["SERVER_ADMIN"]=>
  string(14) "root@localhost"
  ["CONTEXT_DOCUMENT_ROOT"]=>
  string(38) "/var/www/vhosts/quantabio.com/httpdocs"
  ["CONTEXT_PREFIX"]=>
  string(0) ""
  ["REQUEST_SCHEME"]=>
  string(5) "https"
  ["DOCUMENT_ROOT"]=>
  string(38) "/var/www/vhosts/quantabio.com/httpdocs"
  ["REMOTE_ADDR"]=>
  string(10) "3.239.6.58"
  ["SERVER_PORT"]=>
  string(3) "443"
  ["SERVER_ADDR"]=>
  string(13) "172.31.63.191"
  ["SERVER_NAME"]=>
  string(17) "www.quantabio.com"
  ["SERVER_SIGNATURE"]=>
  string(0) ""
  ["HTTP_HOST"]=>
  string(17) "www.quantabio.com"
  ["HTTP_X_REAL_IP"]=>
  string(13) "185.93.229.32"
  ["HTTP_X_FORWARDED_FOR"]=>
  string(10) "3.239.6.58"
  ["HTTP_CONNECTION"]=>
  string(5) "close"
  ["HTTP_X_FORWARDED_PROTO"]=>
  string(5) "https"
  ["HTTP_X_SUCURI_CLIENTIP"]=>
  string(10) "3.239.6.58"
  ["HTTP_X_SUCURI_COUNTRY"]=>
  string(2) "US"
  ["HTTP_USER_AGENT"]=>
  string(40) "CCBot/2.0 (https://commoncrawl.org/faq/)"
  ["HTTP_ACCEPT"]=>
  string(63) "text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8"
  ["HTTP_ACCEPT_LANGUAGE"]=>
  string(14) "en-US,en;q=0.5"
  ["HTTP_ACCEPT_ENCODING"]=>
  string(7) "br,gzip"
  ["SSL_TLS_SNI"]=>
  string(17) "www.quantabio.com"
  ["HTTPS"]=>
  string(2) "on"
  ["HTTP_AUTHORIZATION"]=>
  string(0) ""
  ["SCRIPT_URI"]=>
  string(114) "https://www.quantabio.com/product-category/products/pcr/real-time-quantitative-pcr/rna-real-time-quantitative-pcr/"
  ["SCRIPT_URL"]=>
  string(89) "/product-category/products/pcr/real-time-quantitative-pcr/rna-real-time-quantitative-pcr/"
  ["UNIQUE_ID"]=>
  string(27) "ZCenlZbsdD4lQnxzl1B-pAAAAAU"
  ["REDIRECT_STATUS"]=>
  string(3) "200"
  ["REDIRECT_SSL_TLS_SNI"]=>
  string(17) "www.quantabio.com"
  ["REDIRECT_HTTPS"]=>
  string(2) "on"
  ["REDIRECT_HTTP_AUTHORIZATION"]=>
  string(0) ""
  ["REDIRECT_SCRIPT_URI"]=>
  string(114) "https://www.quantabio.com/product-category/products/pcr/real-time-quantitative-pcr/rna-real-time-quantitative-pcr/"
  ["REDIRECT_SCRIPT_URL"]=>
  string(89) "/product-category/products/pcr/real-time-quantitative-pcr/rna-real-time-quantitative-pcr/"
  ["REDIRECT_UNIQUE_ID"]=>
  string(27) "ZCenlZbsdD4lQnxzl1B-pAAAAAU"
  ["FCGI_ROLE"]=>
  string(9) "RESPONDER"
  ["PHP_SELF"]=>
  string(10) "/index.php"
  ["REQUEST_TIME_FLOAT"]=>
  float(1680320405.4246)
  ["REQUEST_TIME"]=>
  int(1680320405)
  ["SUCURIREAL_REMOTE_ADDR"]=>
  string(13) "185.93.229.32"
}
Quantabio Logo
  • Product Finder
  • Products
    • All Products
    • Sample Preparation
      • DNA
        • Extracta Plus DNA
        • Extracta DBS
        • Extracta DNA Prep for PCR
        • Phase Lock Gel
      • RNA
        • Extracta Plus RNA
        • Phase Lock Gel
    • Reverse Transcription
      • First-Strand cDNA Synthesis
        • qScript Ultra SuperMix
        • qScript Ultra Flex Kit
        • qScript cDNA SuperMix
        • qScript XLT cDNA SuperMix
        • qScript cDNA Synthesis Kit
        • qScript Flex cDNA Synthesis Kit
    • PCR
      • DNA Amplification
        • AccuStart II PCR SuperMix
        • AccuStart II GelTrack PCR SuperMix
        • sparQ HiFi PCR Master Mix
        • AccuStart II PCR ToughMix
        • repliQa HiFi ToughMix
        • AccuStart Long Range SuperMix
        • AccuStart II PCR Genotyping Kit
      • RNA Amplification
        • qScript XLT 1-Step RT-PCR Kit
    • Real-Time qPCR
      • DNA Amplification
        • SYBR Green Detection
          • PerfeCTa SYBR® Green SuperMix
          • PerfeCTa SYBR® Green FastMix
        • Probe-based Detection
          • AccuStart Genotyping ToughMix
          • PerfeCTa qPCR FastMix UNG
          • PerfeCTa FastMix II
          • PerfeCTa qPCR ToughMix
          • PerfeCTa Multiplex qPCR ToughMix
          • PerfeCTa Multiplex qPCR SuperMix
      • RNA Amplification
        • Qscript lyo 1-step
        • qScript XLT 1-Step RT-qPCR ToughMix
        • qScript One-Step SYBR® Green RT-qPCR
        • qScript One-Step RT-qPCR Kit
        • UltraPlex 1-Step ToughMix
        • qScript 1-Step Virus ToughMix
    • Next Generation Sequencing
      • DNA Library Prep
        • sparQ DNA Frag & Library Prep Kit
        • sparQ DNA Library Prep Kit
        • sparQ UDI Adapters (1-96)
      • RNA Library Prep
        • sparQ RNA-Seq HMR Kit
        • sparQ UDI Adapters (1-96)
      • CleanUp & Size Selection
      • Library Amplification
      • Library Quantification
    • qPCR Instrument
  • Markets & Applications
    • Overview
    • Markets
    •       COVID-19 Testing
    •       Newborn Screening
    •       Pathogen Detection
    •       Plant Testing
    •       Environmental Testing
    •       Food Testing
    •       Animal Health
    •       Craft Beer Testing
    • Applications
    •       Genotyping
    •       Next Generation Sequencing
    •       Gene Expression Analysis
    •       PCR & qPCR
  • Resources
    • Overview
    • Product Flyers
    • Product Manuals
    • Technical Notes
    • FAQs
    • Publications
    • Safety Data Sheets (SDS)
    • CofA (PSF)
    • Brochures
    • Customer Profile Stories
    • Q Cycler Tutorials
    • Protocol Tutorials
    • Webinars
  • About Us
    • About Us
    • Sustainability
    • News
    • Events
  • Contact Us
    • Contact Us
    • Career
    • Global Distributors
  • Products
    • All Products
    • Sample Preparation
      • DNA
        • Extracta Plus DNA
        • Extracta DBS
        • Extracta DNA Prep for PCR
        • Phase Lock Gel
      • RNA
        • Extracta Plus RNA
        • Phase Lock Gel
    • Reverse Transcription
      • First-Strand cDNA Synthesis
        • qScript Ultra SuperMix
        • qScript Ultra Flex Kit
        • qScript cDNA SuperMix
        • qScript XLT cDNA SuperMix
        • qScript cDNA Synthesis Kit
        • qScript Flex cDNA Synthesis Kit
    • PCR
      • DNA Amplification
        • AccuStart II PCR SuperMix
        • AccuStart II GelTrack PCR SuperMix
        • sparQ HiFi PCR Master Mix
        • AccuStart II PCR ToughMix
        • repliQa HiFi ToughMix
        • AccuStart Long Range SuperMix
        • AccuStart II PCR Genotyping Kit
      • RNA Amplification
        • qScript XLT 1-Step RT-PCR Kit
    • Real-Time qPCR
      • DNA Amplification
        • SYBR Green Detection
          • PerfeCTa SYBR® Green SuperMix
          • PerfeCTa SYBR® Green FastMix
        • Probe-based Detection
          • AccuStart Genotyping ToughMix
          • PerfeCTa qPCR FastMix UNG
          • PerfeCTa FastMix II
          • PerfeCTa qPCR ToughMix
          • PerfeCTa Multiplex qPCR ToughMix
          • PerfeCTa Multiplex qPCR SuperMix
      • RNA Amplification
        • Qscript lyo 1-step
        • qScript XLT 1-Step RT-qPCR ToughMix
        • qScript One-Step SYBR® Green RT-qPCR
        • qScript One-Step RT-qPCR Kit
        • UltraPlex 1-Step ToughMix
        • qScript 1-Step Virus ToughMix
    • Next Generation Sequencing
      • DNA Library Prep
        • sparQ DNA Frag & Library Prep Kit
        • sparQ DNA Library Prep Kit
        • sparQ UDI Adapters (1-96)
      • RNA Library Prep
        • sparQ RNA-Seq HMR Kit
        • sparQ UDI Adapters (1-96)
      • CleanUp & Size Selection
      • Library Amplification
      • Library Quantification
    • qPCR Instrument
  • Markets & Applications
    • Overview
    • Markets
    •       COVID-19 Testing
    •       Newborn Screening
    •       Pathogen Detection
    •       Plant Testing
    •       Environmental Testing
    •       Food Testing
    •       Animal Health
    •       Craft Beer Testing
    • Applications
    •       Genotyping
    •       Next Generation Sequencing
    •       Gene Expression Analysis
    •       PCR & qPCR
  • Resources
    • Overview
    • Product Flyers
    • Product Manuals
    • Technical Notes
    • FAQs
    • Publications
    • Safety Data Sheets (SDS)
    • CofA (PSF)
    • Brochures
    • Customer Profile Stories
    • Q Cycler Tutorials
    • Protocol Tutorials
    • Webinars
  • About Us
    • About Us
    • Sustainability
    • News
    • Events
  • Contact Us
    • Contact Us
    • Career
    • Global Distributors
  • Product Finder

RNA

Home / Products / PCR / Real-Time Quantitative PCR / RNA

Quantitative RT-qPCR comparison chart

  Quantitative RT-qPCR
  Probe-Based Detection SYBR Green Detection
Qscript lyo 1-step qScript XLT 1-Step RT-qPCR ToughMix qScript One-Step RT-qPCR Kit UltraPlex 1-Step ToughMix qScript 1-Step Virus ToughMix qScript One-Step SYBR Green RT-qPCR
Features
Kit Format One Tube One Tube Two Tubes One Tube One Tube Two Tubes
RT Enzyme  MMLV, reduced RNase activity MMLV, reduced RNase activity MMLV, RNase H+ MMLV, reduced RNase activity MMLV, reduced RNase activity MMLV, RNase H+
Concentration  N/A 2x 2x 4x 2x 2x
Yield ++++ ++++ +++ ++++ ++++ +++
Total RNA Input Range  0.5pg – 500 pg 1pg – 100ng 1pg – 1ug 1pg – 100ng 1pg – 100ng 1pg – 100ng
Amplicon Length 70 – 300bp 70 – 300bp 70 – 300bp 70 – 300bp 70 – 300bp 70 – 200bp
Hot Start N/A N/A N/A Yes Yes N/A
Applications
Inhibitor Tolerant    •   • •  
High Yield    •   • •  
Multiplex PCR  • •   • •  
Available Formats
Master Mix • •   • •  
Lyophilized •          
Packaging (Rxns/Units) 8 Rxns
24 Rxns
100 Rxns
500 Rxns
2000 Rxns
50 Rxns
200 Rxns
100 Rxns
500 Rxns
1000 Rxns
100 Rxns
500 Rxns
2000 Rxns
100 Rxns
400 Rxns

All Products

Showing 1–5 of 7 results

  • AccuStart Genotyping ToughMix

    Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. This proprietary formulation has been rigorously optimized to destabilize single base-pair mismatches to ensure precise…
    Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional…
    Details
    Details
  • qScript 1-Step Virus ToughMic

    qScript 1-Step Virus ToughMix

    qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable…
    qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B,…
    Details
    Details
  • Qscript lyo 1-step

    Qscript lyo 1-step is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes. The reagent contains a hot-start thermo-stable polymerase, a genetically engineered reverse transcriptase as well as other components to ensure higher performance…
    Qscript lyo 1-step is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes. The…
    Details
    Details
  • qScript One-Step RT-qPCR Kit

    The qScript One-Step qRT-PCR Kit is a convenient and highly sensitive solution for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan(r) 5'-hydrolysis probes.  cDNA Synthesis and PCR amplification are carried out in…
    The qScript One-Step qRT-PCR Kit is a convenient and highly sensitive solution for reverse transcription quantitative PCR (RT-qPCR) of RNA…
    Details
    Details
  • qScript One-Step SYBR® Green RT-qPCR

    The qScript One-Step SYBR® Green RT-qPCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (RT-qPCR) using SYBR Green I dye detection and gene-specific primers. cDNA synthesis and PCR amplification are carried out in the…
    The qScript One-Step SYBR® Green RT-qPCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates…
    Details
    Details
  • 1
  • 2
  • →

Product Finder

Select Your Assay

Next

Starting Template

Back Next

Assay Format

Back Next

Detection Chemistry

Back Next

Multiplexing (more than 3 targets)

Back Next

Is gene-specific priming (GSP) required?

Back Next

What current Reverse Transcriptase or cDNA kit are you using?

Back Next

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
Back Next

Choose your application from the categories below

Back Next

Products

Back Next
I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
* Required information
Back
Products
  • Sample Preparation
  • Reverse Transcription
  • PCR
  • Real-Time Quantitative PCR
  • Next Generation Sequencing
  • qPCR Instrument
Markets & Applications
  • COVID-19 Testing
  • Newborn Screening
  • Next Generation Sequencing
  • PCR & qPCR
Quantabio
  • Contact Us
  • Company
  • News
  • Events
  • Resources
Stay Connected
Privacy Policy Site Map Terms & Conditions
Quantabio © 2023. All rights reserved.