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AccuStart II PCR Genotyping Kit

Completely reagent-based system enables reliable PCR genotyping with minimal pipetting skill
Features & Benefits
  • Simplified, completely reagent-based system requires minimal pipetting skill
  • Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X)
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
  • Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification

 

AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Order Info
Product
Kit Size
Order Info
AccuStart II PCR Genotyping Kit
Request Sample
Kit Size:
Order Info:
100 x 25 μL rxns (6)
500 x 25 μL rxns

Description

The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (? 100uL) and can be carried out in ? 30-minutes on a standard PCR thermal cycler.

What Customers Say

We have tested several different PCR Mastermixes for genotyping in the past four months. Accustart II was the most convincing one concerning quality and price.”

Medical University of Vienna
Research Scientist
Details

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).
Customer Testimonials

Customer Testimonials

AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. |
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).

Performance Data

Resources

Publications

Inflammation and CB2 signaling drive novel changes in the ocular lipidome and regulate immune cell activity in the eye
JT Toguri - 2018
Abstract
Uveitis is inflammation of the uvea which consists of the iris, ciliary body and the choroid of the eye. Uveitis can lead to impaired vision and is responsible for 10% of all cases of blindness globally. Using an endotoxin-induced uveitis (EIU) rodent model, our previous data implicated the endogenous cannabinoid system (ECS) in the amelioration of many of the components of the inflammatory response. Here, we test the hypothesis that the reduction in inflammatory mediators in the EIU model by the CB2 agonist, HU308, is associated with changes in ECS endogenous ligands as well as related lipids, prostaglandins (PGs), 2-acyl glycerols, and lipoamines. Analysis of leukocytes and neutrophils, CB2 mRNA, and 26 lipids in the eye of WT mice after EIU induction and HU308 treatment were compared to the same analyses in the CB2 knock-out (CB2 KO) mouse. Endothelial leukocyte adhesion and neutrophil migration were significantly increased in both WT and CB2 KO after EIU. HU308 significantly reduced the leukocyte adhesion and neutrophil recruitment in the WT animals. HU308 also significantly reduced leukocyte adhesion in the CB2 KO mouse, yet, had no effect on neutrophil infiltration suggesting an important off-target effect of HU308. Lipidomics profiles revealed significant increases in 6 non-ECS lipids after EIU in the WT and that HU308 effectively reduced these back to control levels; in addition, HU308 increased levels of 2-acyl glycerols and decreased all N-acyl glycines. CB2 KOs with saline-injection compared to WT had significantly elevated levels of 2-acyl glycerols, whereas levels of N-oleoyl ethanolamine (OEA), N-stearoyl ethanolamine (SEA), and PGE2 were reduced. CB2 KOs with EIU had 13 lipids that were significantly lower than WT with EIU including 4 N-acyl glycines. HU308 had no effect on lipid concentrations in the CB2 KOs with EIU, however, it did cause further reductions on 3 additional lipids compared to saline controls. HU308 appears to be acting at a non-CB2 target for the reduction of leukocyte infiltration in the EIU model; however, our data suggest that HU308 is working through CB2 to reduce neutrophil migration and for the regulation of multiple lipid signaling pathways including PGs, lipoamines, and 2-acyl glycerols. These data implicate ocular CB2 as a key component of lipid signaling in the eye and part of the regulatory processes of inflammation.
Single-Nucleotide Polymorphisms in IL23R-IL12RB2 (rs1495965) Are Highly Prevalent in Patients with Behcet’s Uveitis, and Vary between Populations
Michal Kramer - 2018
Abstract
Purpose: To test the frequency of single-nucleotide polymorphisms in the IL-10, IL23R-IL12RB2 genes in patients with Behcet’s uveitis.Methods: Blood samples were collected from 89 Israeli and Turkish patients, and from healthy control subjects of different origins. Genomic DNA was extracted from peripheral blood leukocytes and genotyped.Results: The risk allele, A, in rs1800871, of IL-10 gene was highly prevalent in Behcet’s uveitis and healthy control samples alike; highest among the Turkish groups. Prevalence of G allele, in rs1495965, in the IL23R-IL12RB2 gene was high in Behcet’s uveitis patients, and among healthy Turkish and Israelis of Middle Eastern origin, while lower among the other Israeli control group (77.9%, 78.9%, 27.8%, respectively, P < 0.001).Conclusion: Our findings highlight the differences between populations and may account for the increased prevalence of the disease among Turkish and Israelis of Middle Eastern origin. Further studies are required to map other healthy and affected populations.
Core Binding Factor β Expression in Ovarian Granulosa Cells Is Essential for Female Fertility
Somang Lee-Thacker - 2018
Abstract
Core binding factor β (CBFβ) is a non–DNA-binding partner of all RUNX proteins and critical for transcription activity of CBF transcription factors (RUNXs/CBFβ). In the ovary, the expression of Runx1 and Runx2 is highly induced by the luteinizing hormone (LH) surge in ovulatory follicles, whereas Cbfb is constitutively expressed. To investigate the physiological significance of CBFs in the ovary, the current study generated two different conditional mutant mouse models in which granulosa cell expression of Cbfb and Runx2 was reduced by Cre recombinase driven by an Esr2 promoter. Cbfbgc−/− and Cbfbgc−/− × Runx2gc+/− mice exhibited severe subfertility and infertility, respectively. In the ovaries of both mutant mice, follicles develop normally, but the majority of preovulatory follicles failed to ovulate either in response to human chorionic gonadotropin administration in pregnant mare serum gonadotropin–primed immature animals or after the LH surge at 5 months of age. Morphological and physiological changes in the corpus luteum of these mutant mice revealed the reduced size, progesterone production, and vascularization, as well as excessive lipid accumulation. In granulosa cells of periovulatory follicles and corpora lutea of these mice, the expression of Edn2, Ptgs1, Lhcgr, Sfrp4, Wnt4, Ccrl2, Lipg, Saa3, and Ptgfr was also drastically reduced. In conclusion, the current study provided in vivo evidence that CBFβ plays an essential role in female fertility by acting as a critical cofactor of CBF transcription factor complexes, which regulate the expression of specific key ovulatory and luteal genes, thus coordinating the ovulatory process and luteal development/function in mice.
The Adhesion G-Protein-Coupled Receptor, GPR56/ADGRG1, Inhibits Cell–Extracellular Matrix Signaling to Prevent Metastatic Melanoma Growth
Michelle W. Millar - 2018
Abstract
Metastatic growth is considered a rate-limiting step in cancer progression, and upregulation of extracellular matrix (ECM) deposition and cell–ECM signaling are major drivers of this process. Mechanisms to reverse ECM upregulation in cancer could potentially facilitate its prevention and treatment but they are poorly understood. We previously reported that the adhesion G-protein-coupled receptor GPR56/ADGRG1 is downregulated in melanoma metastases. Its re-expression inhibited melanoma growth and metastasis and reduced the deposition of fibronectin, a major ECM component. We hypothesize that its effect on fibronectin deposition contributes to its inhibitory role on metastatic growth. To test this, we investigated the function of GPR56 on cell–fibronectin adhesion and its relationship with metastatic growth in melanoma. Our results reveal that GPR56 inhibits melanoma metastatic growth by impeding the expansion of micrometastases to macrometastases. Meanwhile, we present evidence that GPR56 inhibits fibronectin deposition and its downstream signaling, such as phosphorylation of focal adhesion kinase (FAK), during this process. Administration of the FAK inhibitor Y15 perturbed the proliferation of melanoma metastases, supporting a causative link between the cell adhesion defect induced by GPR56 and its inhibition of metastatic growth. Taken together, our results suggest that GPR56 in melanoma metastases inhibits ECM accumulation and adhesion, which contributes to its negative effects on metastatic growth.

Customer Testimonials

AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. |
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

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