US

Extracta Plus RNA

Rapid extraction and purification of high-quality total RNA from cultured cells or tissue
Features & Benefits
  • Rapid Protocol – From cells or tissue samples to high-quality total RNA in just 25 mins
  • Streamlined gDNA removal – Novel DNA removal column eliminates gDNA contamination and need for DNase treatment
  • Extraction of low input quantities – Ability to extract RNA from as little as 10 cells
  • Downstream compatibility – Ideal for applications such as RT-PCR, RT-qPCR and next generation sequencing

Extracta Plus RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Part Number
Price
Quantity
 
Product
Kit Size
Order Info
Price
Quantity
Extracta Plus RNA
Request Sample
Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  10 rxn
Part Number:  95214-010
Price:  $77.00
Add to Cart
Kit Size:  50 rxn
Part Number:  95214-050
Price:  $388.00
Add to Cart

Description

The Extracta Plus RNA kit provides rapid extraction and purification of RNA from cultured human or animal cells and from various tissue samples. The Extracta Plus spin column workflow enables simultaneous processing of multiple samples in 25 mins to yield purified RNA suitable for a range of downstream applications. The kit includes specially designed Extracta Plus DNA removal columns for effective removal of genomic DNA contamination. This provides a convenient solution for applications that are sensitive to low amounts of DNA contamination such as RT-qPCR and NGS.

Details

Details

Contents

 

Volume

Component Description

10 Reactions

50 Reactions

Buffer EPRL

6 ml

45 ml

Buffer EPRW1

7 ml

45 ml

Buffer EPRW2

3 ml

11 ml

RNase-free water

10 ml

10 ml

Extracta Plus Spin Column

10 piece

50 piece

Extracta Plus DNA Removal Column

10 piece

50 piece

Collection Tubes 1.5 ml

10 piece

50 piece

Collection Tubes 2 ml

10 piece

50 piece

Details

Contents

 

Volume

Component Description

10 Reactions

50 Reactions

Buffer EPRL

6 ml

45 ml

Buffer EPRW1

7 ml

45 ml

Buffer EPRW2

3 ml

11 ml

RNase-free water

10 ml

10 ml

Extracta Plus Spin Column

10 piece

50 piece

Extracta Plus DNA Removal Column

10 piece

50 piece

Collection Tubes 1.5 ml

10 piece

50 piece

Collection Tubes 2 ml

10 piece

50 piece

Performance Data

Resources

Publications

De novo transcriptome assembly and gene annotation for the toxic dinoflagellate Dinophysis
Chetan C. Gaonkar - 2023
Abstract
Species within the dinoflagellate genus Dinophysis can produce okadiac acid and dinophysistoxins leading to diarrhetic shellfish poisoning. Since the first report of D. ovum from the Gulf of Mexico in 2008, reports of other Dinophysis species across US have increased. Members of the D. cf. acuminata complex (D. acuminata, D. acuta, D. ovum, D. sacculus) are difficult to differentiate due to their morphological similarities. Dinophysis feeds on and steals the chloroplasts from the ciliate, Mesodinium rubrum, which in turn has fed on and captured the chloroplasts of its prey, the cryptophyte Teleaulax amphioxeia. The objective of this study was to generate de novo transcriptomes for new isolates of these mixotrophic organisms. The transcriptomes obtained will serve as a reference for future experiments to assess the effect of different abiotic and biotic conditions and will also provide a useful resource for screening potential marker genes to differentiate among the closely related species within the D. cf. acuminata-complex. The complete comprehensive detailed workflow and links to obtain the transcriptome data are provided.

Welcome to the Quantabio webshop!

Please complete the new user account registration form

Once your account is setup, you'll be able to purchase any of the products on our site at any time with next day delivery for in stock products.

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
* Required information