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qScript Ultra Flex Kit

Faster, longer, tougher next gen cDNA synthesis with flexible priming
Features & Benefits
  • Enhanced Thermostability – Engineered for RT activity up to 65°C, overcoming challenging secondary structures
  • Superior Speed & Ease of Use – 10 minute reaction time with fewer pipetting steps
  • Maximum Yield & Sensitivity – Improved yields with limiting or compromised samples
  • Ultimate Inhibitor Resistance – Overcome a wide array of PCR inhibitors (salt, heparin, hematin, etc.)
  • Longer Transcripts – Ability to reverse transcribe single targets up to 20 kb

qScript Ultra flexKit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Order Info
Product
Kit Size
Order Info
qScript Ultra Flex Kit
Request Sample
Kit Size:
Order Info:
95215-025
95215-100

Description

qScript Ultra Flex Kit is easy-to-use and highly efficient for the synthesis of first-strand cDNA with flexible priming methods including oligo-dT, random primers and gene specific priming to reverse transcribe RNA to cDNA. A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase engineered for improved thermostability, velocity, processivity, and resistance to many common reaction inhibitors. qScript Ultra Flex Kit contains all required components for first-strand cDNA synthesis except RNA template and gene specific primers. The cDNA product is compatible with downstream 2-step RT-qPCR or RT-PCR procedures.

Details

Details

Contents

Components

95215-025

95215-100

5x qScript Ultra Reaction Mix

Optimized master mix containing buffer, magnesium, dNTPs and qScript Ultra RT

1 x 100 µL

1 x 400 µL

Oligo(dT) primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

Random primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

GSP Enhancer (10x)

1 x 50 µL

1 x 200 µL

Nuclease-free Water

1 x 1.5 mL

2 x 1.5 mL

Details

Contents

Components

95215-025

95215-100

5x qScript Ultra Reaction Mix

Optimized master mix containing buffer, magnesium, dNTPs and qScript Ultra RT

1 x 100 µL

1 x 400 µL

Oligo(dT) primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

Random primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

GSP Enhancer (10x)

1 x 50 µL

1 x 200 µL

Nuclease-free Water

1 x 1.5 mL

2 x 1.5 mL

Performance Data

Resources

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

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