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qScript Ultra Flex Kit

Faster, longer, tougher next gen cDNA synthesis with flexible priming
Features & Benefits
  • Enhanced Thermostability – Engineered for RT activity up to 65°C, overcoming challenging secondary structures
  • Superior Speed & Ease of Use – 10 minute reaction time with fewer pipetting steps
  • Maximum Yield & Sensitivity – Improved yields with limiting or compromised samples
  • Ultimate Inhibitor Resistance – Overcome a wide array of PCR inhibitors (salt, heparin, hematin, etc.)
  • Longer Transcripts – Ability to reverse transcribe single targets up to 20 kb

qScript Ultra Flex Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Product
Kit Size
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Price
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qScript Ultra Flex Kit
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Quantity:
Kit Size:  25 x 20 µL reactions
Part Number:  95215-025
Price:  $201.00
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Kit Size:  100 x 20 µL reactions
Part Number:  95215-100
Price:  $633.00
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Description

qScript Ultra Flex Kit is easy-to-use and highly efficient for the synthesis of first-strand cDNA with flexible priming methods including oligo-dT, random primers and gene specific priming to reverse transcribe RNA to cDNA. A key component is a novel, state-of-the-art, RNase H deficient reverse transcriptase engineered for improved thermostability, velocity, processivity, and resistance to many common reaction inhibitors. qScript Ultra Flex Kit contains all required components for first-strand cDNA synthesis except RNA template and gene specific primers. The cDNA product is compatible with downstream 2-step RT-qPCR or RT-PCR procedures.

Details

Details

Contents

Components

95215-025

95215-100

5x qScript Ultra Reaction Mix

Optimized master mix containing buffer, magnesium, dNTPs and qScript Ultra RT

1 x 100 µL

1 x 400 µL

Oligo(dT) primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

Random primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

GSP Enhancer (10x)

1 x 50 µL

1 x 200 µL

Nuclease-free Water

1 x 1.5 mL

2 x 1.5 mL

Details

Contents

Components

95215-025

95215-100

5x qScript Ultra Reaction Mix

Optimized master mix containing buffer, magnesium, dNTPs and qScript Ultra RT

1 x 100 µL

1 x 400 µL

Oligo(dT) primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

Random primers

10x concentrated in Enhancer solution

1 x 50 µL

1 x 200 µL

GSP Enhancer (10x)

1 x 50 µL

1 x 200 µL

Nuclease-free Water

1 x 1.5 mL

2 x 1.5 mL

Performance Data

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