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PerfeCTa qPCR FastMix UNG

Features & Benefits
  • Fast Cycling protocols
  • contains UNG to eliminate amplification of carry-over contamination

 

PerfeCTa qPCR FastMix UNG Kits are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Perfecta qPCR FastMix UNG

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Kit Size:  1250 x 20 μL rxns (10 x 1.25 mL)
Part Number:  95076-012
Price:  $1,012.00
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Kit Size:  5000 x 20 μL rxns (1 x 50 mL)
Part Number:  95076-05K
Price:  $2,531.00
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Perfecta qPCR FastMix UNG ROX

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Kit Size:  1250 x 20 μL rxns (10 x 1.25 mL)
Part Number:  95077-012
Price:  $1,012.00
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Kit Size:  5000 x 20 μL rxns (1 x 50 mL)
Part Number:  95077-05K
Price:  $2,531.00
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Perfecta qPCR FastMix UNG Low-ROX

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Kit Size:  1250 x 20 μL rxns (10 x 1.25 mL)
Part Number:  95078-012
Price:  $1,012.00
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Kit Size:  5000 x 20 μL rxns (1 x 50 mL)
Part Number:  95078-05K
Price:  $2,501.00
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Description

PerfeCTa qPCR FastMix, UNG is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers, probe(s), and template for real-time quantitative PCR systems that do not require an internal reference dye. The proprietary buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal with TaqMan® or TaqMan MGB probe chemistry when using rapid PCR cycle times and reduced reaction volumes. This affords greater reagent economy and laboratory throughput on conventional or rapid ramp rate qPCR systems. The enhanced specificity of this FastMix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this FastMix is AccuFast™ Taq DNA polymerase. This hot-start Taq contains a proprietary mixture of monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step (> 48 hours at room temperature). Similar to our AccuStart™ Taq DNA polymerase, these antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast Taq is instantaneous at 95ºC. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200 bp is complete in less than 20s at 60ºC. Additionally, the dNTP mix in this FastMix contains dUTP in place of dTTP. Inclusion of uracil-N-glycosylase (UNG) prevents amplification of carry-over contamination from previous dU-containing PCRs.

Details

Details

Contents

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, AccuFast Taq DNA Polymerase, UNG, and stabilizers

Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Details

Contents

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, AccuFast Taq DNA Polymerase, UNG, and stabilizers

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Resources

Product Manuals

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

FAQs

What are the advantages of PerfeCTa qPCR FastMix vs PerfeCTa qPCR Supermix?
Amplification can be run in about X minutes, at least 3X faster than standard runs with the PerfeCTa qPCR Supermix, with comparable results. For more information on using these reagents, please consult the PerfeCTa qPCR FastMix and PerfeCTa qPCR Supermix Protocol.
Storage of PerfeCTa qPCR FastMix
We recommend a storage temperature of 2 – 8 ºC for the TaqMan® Fast Universal PCR Master Mix (P/N 4352042). The shelf life is 9 months from the manufacturing date.
SNP assays with PerfeCTa qPCR FastMix?
The TaqMan® SNP Genotyping assays have not been fully tested on the Applied Biosystems 7500 Fast System.
What component in the PerfeCTa qPCR FastMix allows rapid amplification results?
The hot-start DNA polymerase enzyme system in the PerfeCTa qPCR FastMix only requires a 30 seconds activation step, and supports significantly shorter hold times for the thermal cycling stages of PCR, thereby minimizing PCR run time. For more information on using this reagent, please consult the PerfeCTa qPCR FastMix (2X) protocol.
How do assays performed with PerfeCTa qPCR FastMix compare with assays performed with PerfeCTa qPCR Supermix?
In general, assays have been found to perform comparably. Please consult the PerfeCTa qPCR Supermix (2X) Protocol for more information on using this reagent.
What are the applications for the PerfeCTa qPCR FastMix?
Absolute Quantitation and Relative Quantitation assays can be run fast. Allelic Discrimination (SNP) assays and plus/minus assays using internal positive controls cannot be run fast.
How stable is an experiment set up with the PerfeCTa qPCR Fastmix Kit at room temperature?
For optimal results, it is recommended to directly run the reaction plate after setting up the reaction. If for any reason a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on a Real-Time PCR System.

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  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
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