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PCR & qPCR
PCR / qPCR

Specific & Efficient Primer Extension

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity. That’s why Quantabio PCR reagents are formulated with ultrapure AccuStart™ DNA polymerase, which contains a stringent antibody hot start to ensure specific and efficient primer extension only after activation at 94°C.

Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Quantabio manufactured reagents:

  • Maximize assay performance
  • Minimize errors
  • Deliver reliable, reproducible results
Workflow Solutions

Cells and tissues

Cells and tissues

DNA Extraction

DNA Extraction

Amplification

Amplification
Detection
Detection
Customer Testimonials
repliQa HiFi ToughMix

"Worked great amplifying two gene target from plasmid DNA. It was the first time I have performed a two step reaction and it worked great. It was awesome running everything in 23 minutes too."

Dr. Brandon L. | University of Nebraska
repliQa HiFi ToughMix

"Worked great amplifying two gene target from plasmid DNA. It was the first time I have performed a two step reaction and it worked great. It was awesome running everything in 23 minutes too."

Dr. Brandon L. | University of Nebraska
repliQa HiFi ToughMix

"I used NEB Q5 for the same plasmid for PCR, same annealing temperature. Only the repliQa HiFi toughMix gave me the correct size products. It is very fast and works much better than NEB Q5 for difficult plasmids in PCR."

Dr. Kara C. | UT Southwestern Medical Center
AccuStart Long Range SuperMix

"The AccuStart Long Range SuperMix produced a clean long-range product (10 kbp) on our first try! We are excited to have a reliable and easy-to-use polymerase to produce DNA for single-molecule studies."

Danielle M. | Professor, Stanford University
PerfeCTa SYBR Green FastMix

"With the PerfeCTa SYBR Green FastMix we had less variability in our triplicates and more reproducible results between repeat experiments."

With the PerfeCTa SYBR Green FastMix we had less variability in our triplicates and more reproducible results between repeat experiments. | Professor, UCSF
PerfeCTa SYBR Green SuperMix

"PerfeCTa SYBR Green SuperMix has been my first and successful experience that boosted my confidence for qPCR. I am really satisfied with its performance."

Teaching Assistant | Ohio University
repliQa HiFi ToughMix

"This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."

Lili C. | Post Doc, USC
repliQa HiFi ToughMix

"The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."

Simon S. | Professor, Tennessee State University

PCR Comparisons

AccuStart II PCR SuperMixAccuStart II GelTrack PCR SuperMixsparQ HiFi PCR Master MixAccuStart II PCR ToughMixrepliQa HiFi ToughMixAccuStart Long Range SuperMix
Features
Concentration2x2x2x2x2x4x
Amplicon SizeUp to 4 kbUp to 4 kbUp to 4 kbUp to 4 kbUp to 24 kbUp to 24 kb
Extension Time*60 sec/kb60 sec/kb30 sec/kb60 sec/kb1-10 sec/kb30-60 sec/kb
Multiplex PCRUp to 6 Targets
Fidelity vs. Taq1x1x80x Taq1x90x Taq10-12x Taq
dU TolerantNoNoNoNoYesYes
5' - 3' exoxxN/AxN/AN/A
3' - 5' exoN/AN/AxN/Axx
Resulting Ends3' dA overhang3' dA overhangBlunt3' dA overhangBluntBlunt/T overhangs
Applications
Routine PCRxxxxx
High Fidelityx**x
Crude PCRx**
Fast PCR**
Long PCRx**
High Yieldxx**x
NGS Library Amplification**x
Available Formats
Hot Startxxxxxx
Master Mixxxxxxx
Loading Dye Availablexx
Packaging (Rxns/Units)100 Rxns 500 Rxns 4000 Rxns100 Rxns 500 Rxns 4000 Rxns50 Rxns 250 Rxns100 Rxns 800 Rxns 4000 Rxns25 Rxns 100 Rxns 500 Rxns25 Rxns 100 Rxns

qPCR Comparisons

PerfeCTa SYBR Green SuperMixPerfeCTA SYBR Green FastMixAccuStart Genotyping ToughMixPerfeCTa qPCR FastMix UNGPerfeCTa FastMix IIPerfeCTa qPCR ToughMixPerfeCTa qPCR ToughMix, UNGPerfeCTa Multiplex qPCR ToughMixPerfeCTa Multiplex qPCR SuperMix
Features
Concentration2x2x2x2x2x2x2x5x2x
Sample TypegDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNAgDNA, cDNA
Fast CyclingNoYesYesYesYesYesYesYesYes
Multiplex CompatibilityN/AN/AUp to 2 targetsUp to 2 targetsUp to 2 targetsUp to 2 targetsUp to 2 targetsUp to 5 targetsUp to 4 targets
Carryover Contamination ControlN/AN/AN/AHeat-labile UNG & blend of dTTP/dUTPN/AN/AHeat-labile UNG & blend of dTTP/dUTPN/AN/A
Applications
Gene Expressionxxxxxxxx
microRNAxx
ChIPxx
SNP Genotypingx
Inhibitor Tolerantx**xx
High Yieldxxx**
Available Formats
Master Mixxxxxxxxxx
Packaging (Rxns/units)250 Rxns 1250 Rxns 2000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns250 Rxns 1250 Rxns 5000 Rxns50 Rxns 200 Rxns 1000 Rxns

We’re Here to Help

Do you have questions about our products or their applications? Our experts are standing by for support.

Contact Us

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

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