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qScript One-Step RT-qPCR Kit

Features & Benefits
  • 1-step convenience
  • high efficiency and sensitivity
  • available with reference dye pre-mixed

 

qScript One-Step RT-qPCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Price
Quantity
 
Product
Kit Size
Order Info
Price
Quantity

qScript One-Step RT-qPCR Kit

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Price:
Quantity:
Kit Size:  200 x 50 μL rxns
Part Number:  95057-200
Price:  $758.00
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qScript One-Step qRT-PCR Kit, Low-ROX

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Kit Size:  200 x 50 μL rxns (2)
Part Number:  95059-200
Price:  $743.00
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qScript One-Step qRT-PCR Kit, ROX

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Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  200 x 50 μL rxns
Part Number:  95058-200
Price:  $758.00
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Description

The qScript One-Step qRT-PCR Kit is a convenient and highly sensitive solution for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan(r) 5'-hydrolysis probes.  cDNA Synthesis and PCR amplification are carried out in the same tube without opening between procedures.  The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes.  The proprietary reaction buffer has been specifically formulated to maximize activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for primer-dimer and other non-specific PCR artifacts.

Details

Details

Contents

qScript One-Step Reverse Transcriptase (50x)

One-Step Master Mix 2x (optionally containing ROX or Low ROX)

Nuclease-free water

Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Details

Contents

qScript One-Step Reverse Transcriptase (50x)

One-Step Master Mix 2x (optionally containing ROX or Low ROX)

Nuclease-free water

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Resources

Product Manuals

Application Notes

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

FAQs

What is the amount of RNA used per TaqMan® or SYBR qPCR Assay?
Suggested input quantities of template are: 1 pg to 1 μg total RNA; 10 fg to 100 ng poly A(+) RNA; 10 to 1x108 copies viral RNA. For more information, please consult the qScrip One-Step qRT-PCR Kits protocols.

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Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

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