US
array(51) {
  ["SERVER_SOFTWARE"]=>
  string(6) "Apache"
  ["REQUEST_URI"]=>
  string(45) "/product/qscript-one-step-sybr-green-rt-qpcr/"
  ["PATH"]=>
  string(49) "/usr/local/sbin:/usr/local/bin:/usr/sbin:/usr/bin"
  ["PP_CUSTOM_PHP_INI"]=>
  string(48) "/var/www/vhosts/system/quantabio.com/etc/php.ini"
  ["PP_CUSTOM_PHP_CGI_INDEX"]=>
  string(19) "plesk-php74-fastcgi"
  ["SCRIPT_NAME"]=>
  string(10) "/index.php"
  ["QUERY_STRING"]=>
  string(0) ""
  ["REQUEST_METHOD"]=>
  string(3) "GET"
  ["SERVER_PROTOCOL"]=>
  string(8) "HTTP/1.1"
  ["GATEWAY_INTERFACE"]=>
  string(7) "CGI/1.1"
  ["REDIRECT_URL"]=>
  string(45) "/product/qscript-one-step-sybr-green-rt-qpcr/"
  ["REMOTE_PORT"]=>
  string(5) "60602"
  ["SCRIPT_FILENAME"]=>
  string(48) "/var/www/vhosts/quantabio.com/httpdocs/index.php"
  ["SERVER_ADMIN"]=>
  string(14) "root@localhost"
  ["CONTEXT_DOCUMENT_ROOT"]=>
  string(38) "/var/www/vhosts/quantabio.com/httpdocs"
  ["CONTEXT_PREFIX"]=>
  string(0) ""
  ["REQUEST_SCHEME"]=>
  string(5) "https"
  ["DOCUMENT_ROOT"]=>
  string(38) "/var/www/vhosts/quantabio.com/httpdocs"
  ["REMOTE_ADDR"]=>
  string(13) "74.132.64.130"
  ["SERVER_PORT"]=>
  string(3) "443"
  ["SERVER_ADDR"]=>
  string(13) "172.31.63.191"
  ["SERVER_NAME"]=>
  string(17) "www.quantabio.com"
  ["SERVER_SIGNATURE"]=>
  string(0) ""
  ["HTTP_COOKIE"]=>
  string(36) "PHPSESSID=md682vnodc6iut2blaislfrj87"
  ["HTTP_REFERER"]=>
  string(62) "https://www.quantabio.com/product/perfecta-sybr-green-fastmix/"
  ["HTTP_USER_AGENT"]=>
  string(46) "Mozilla/4.0 (compatible; MSIE 7.0; Windows NT)"
  ["HTTP_ACCEPT_LANGUAGE"]=>
  string(5) "en-us"
  ["HTTP_ACCEPT"]=>
  string(3) "*/*"
  ["HTTP_X_SUCURI_COUNTRY"]=>
  string(2) "US"
  ["HTTP_X_SUCURI_CLIENTIP"]=>
  string(13) "74.132.64.130"
  ["HTTP_X_FORWARDED_PROTO"]=>
  string(5) "https"
  ["HTTP_CONNECTION"]=>
  string(5) "close"
  ["HTTP_X_FORWARDED_FOR"]=>
  string(13) "74.132.64.130"
  ["HTTP_X_REAL_IP"]=>
  string(13) "185.93.229.32"
  ["HTTP_HOST"]=>
  string(17) "www.quantabio.com"
  ["HTTPS"]=>
  string(2) "on"
  ["HTTP_AUTHORIZATION"]=>
  string(0) ""
  ["SCRIPT_URI"]=>
  string(70) "https://www.quantabio.com/product/qscript-one-step-sybr-green-rt-qpcr/"
  ["SCRIPT_URL"]=>
  string(45) "/product/qscript-one-step-sybr-green-rt-qpcr/"
  ["UNIQUE_ID"]=>
  string(27) "YfGDei8omaC9YqI7jzSSLwAAABA"
  ["REDIRECT_STATUS"]=>
  string(3) "200"
  ["REDIRECT_HTTPS"]=>
  string(2) "on"
  ["REDIRECT_HTTP_AUTHORIZATION"]=>
  string(0) ""
  ["REDIRECT_SCRIPT_URI"]=>
  string(70) "https://www.quantabio.com/product/qscript-one-step-sybr-green-rt-qpcr/"
  ["REDIRECT_SCRIPT_URL"]=>
  string(45) "/product/qscript-one-step-sybr-green-rt-qpcr/"
  ["REDIRECT_UNIQUE_ID"]=>
  string(27) "YfGDei8omaC9YqI7jzSSLwAAABA"
  ["FCGI_ROLE"]=>
  string(9) "RESPONDER"
  ["PHP_SELF"]=>
  string(10) "/index.php"
  ["REQUEST_TIME_FLOAT"]=>
  float(1643217786.188)
  ["REQUEST_TIME"]=>
  int(1643217786)
  ["SUCURIREAL_REMOTE_ADDR"]=>
  string(13) "185.93.229.32"
}

qScript One-Step SYBR Green RT-qPCR

1-step SYBR simplicity
Features & Benefits
  • Sensitive RNA detection with performance-engineered, RNase H (+) M-MLV reverse transcriptase
  • Stringent ultra-pure antibody hot start ensures precise target amplification
  • Flexible buffer chemistry supports either conventional or accelerated thermal cycling conditions

 

qScript One-Step SYBR Green RT-qPCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Order Info
Product
Kit Size
Order Info
qScript One-Step SYBR Green RT-qPCR
Request Sample
Kit Size:
Order Info:
400 x 25 μL rxns (1)
Product
Kit Size
Order Info
qScript One-Step SYBR Green RT-qPCR, Low ROX
Request Sample
Kit Size:
Order Info:
400 x 25 μL rxns (4)

Description

The qScript One-Step SYBR Green RT-qPCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (RT-qPCR) using SYBR Green I dye detection and gene-specific primers. cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures.The proprietary reaction buffer has been specifically formulated to maximize activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for primer-dimer and other non-specific PCR artifacts. This reagent is compatible with both fast and standard qPCR cycling protocols. Precise amplification is essential for successful RT-qPCR with SYBR Green I technology since this dye binds to all dsDNA generated during amplification. This 1-step reagent contains ultra-pure AccuStart™ hot start Taq DNA polymerase that is completely arrested prior to the initial PCR denaturation step. Upon heat activation at 95°C, the antibodies are rapidly and irreversibly denatured, releasing a fully active high-yielding Taq DNA polymerase mutant.
Details

Details

Contents

50X concentrated qScript One-Step Reverse Transcriptase – Optimized 50X formulation of recombinant MMLV reverse transcriptase for one-step RT-PCR.
One-Step SYBR Green Master Mix (2X) – 2X reaction buffer containing dNTPs, magnesium chloride, AccuStart Taq DNA polymerase, stabilizers, and SYBR Green I dye
Nuclease-free water

Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Details

Contents

50X concentrated qScript One-Step Reverse Transcriptase – Optimized 50X formulation of recombinant MMLV reverse transcriptase for one-step RT-PCR.
One-Step SYBR Green Master Mix (2X) – 2X reaction buffer containing dNTPs, magnesium chloride, AccuStart Taq DNA polymerase, stabilizers, and SYBR Green I dye
Nuclease-free water

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Resources

Product Manuals

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

Publications

Autologous Transplantation of Skin-Derived Precursor Cells in a Porcine Model
Anne-Laure Thomas - 2020
Abstract
Background Hirschprung's disease is characterized by aganglionic bowel and often requires surgical resection. Cell-based therapies have been investigated as potential alternatives to restore functioning neurons. Skin-derived precursor cells (SKPs) differentiate into neural and glial cells in vitro and generate ganglion-like structures in rodents. In this report, we aimed to translate this approach into a large animal model of aganglionosis using autologous transplantation of SKPs. Methods Juvenile pigs underwent skin procurement from the shoulder and simultaneous chemical denervation of an isolated colonic segment. Skin cells were cultured in neuroglial-selective medium and labeled with fluorescent dye for later identification. The cultured SKPs were then injected into the aganglionic segments of colon, and the specimens were retrieved within seven days after transplantation. SKPs in vitro and in vivo were assessed with histologic samples for various immunofluorescent markers of multipotency and differentiation. SKPs from the time of harvest were compared to those at the time of injection using PCR. Results Prior to transplantation, 72% of SKPs stained positive for nestin and S100b, markers of neural and glial precursor cells of neural crest origin, respectively. Markers of differentiated neurons and gliocytes, TUJ1 and GFAP, were detected in 47% of cultured SKPs. After transplantation, SKPs were identified in both myenteric and submucosal plexuses of the treated colon. Nestin co-expression was detected in the SKPs within the aganglionic colon in vivo. Injected SKPs appeared to migrate and express early neuroglial differentiation markers. Conclusions Autologous SKPs implanted into aganglionic bowel demonstrated immunophenotypes of neuroglial progenitors. Our results suggest that autologous SKPs may be potentially useful for cell-based therapy for patients with enteric nervous system disorders. Type of Study Basic science.
Co-subsistence of avian influenza virus subtypes of low and high pathogenicity in Bangladesh: Challenges for diagnosis, risk assessment and control
Rokshana Parvin - 2019
Abstract
Endemic co-circulation of potentially zoonotic avian influenza viruses (AIV) of subtypes H5N1 and H9N2 (G1 lineage) in poultry in Bangladesh accelerated diversifying evolution. Two clinical samples from poultry obtained in 2016 yielded five different subtypes (highly pathogenic [HP] H5N1, HP H5N2, HP H7N1, HP H7N2, H9N2) and eight genotypes of AIV by plaque purification. H5 sequences grouped with clade 2.3.2.1a viruses while N1 was related to an older, preceding clade, 2.2.2. The internal genome segments of the plaque-purified viruses originated from clade 2.2.2 of H5N1 or from G1/H9N2 viruses. H9 and N2 segments clustered with contemporary H9N2 strains. In addition, HP H7 sequences were detected for the first time in samples and linked to Pakistani HP H7N3 viruses of 2003. The unexpected findings of mixtures of reassorted HP H5N1 and G1-like H9N2 viruses, which carry genome segments of older clades in association with the detection of HP H7 HA segments calls for confirmation of these results by targeted surveillance in the area of origin of the investigated samples. Hidden niches and obscured transmission pathways may exist that retain or re-introduce genome segments of older viruses or reassortants thereof which causes additional challenges for diagnosis, risk assessment and disease control.
Imaging Mass Spectrometry and Proteome Analysis of Marek’s Disease Virus-Induced Tumors
V. I. Pauker - 2019
Abstract
The highly oncogenic alphaherpesvirus Marek’s disease virus (MDV) causes immense economic losses in the poultry industry. MDV induces a variety of symptoms in infected chickens, including neurological disorders and immunosuppression. Most notably, MDV induces transformation of lymphocytes, leading to T cell lymphomas in visceral organs with a mortality of up to 100%. While several factors involved in MDV tumorigenesis have been identified, the transformation process and tumor composition remain poorly understood. Here we developed an imaging mass spectrometry (IMS) approach that allows sensitive visualization of MDV-induced lymphoma with a specific mass profile and precise differentiation from the surrounding tissue. To identify potential tumor markers in tumors derived from a very virulent wild-type virus and a telomerase RNA-deficient mutant, we performed laser capture microdissection (LCM) and thereby obtained tumor samples with no or minimal contamination from surrounding nontumor tissue. The proteomes of the LCM samples were subsequently analyzed by quantitative mass spectrometry based on stable isotope labeling. Several proteins, like interferon gamma-inducible protein 30 and a 70-kDa heat shock protein, were identified that are differentially expressed in tumor tissue compared to surrounding tissue and naive T cells. Taken together, our results demonstrate for the first time that MDV-induced tumors can be visualized using IMS, and we identified potential MDV tumor markers by analyzing the proteomes of virus-induced tumors. IMPORTANCE Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that infects chickens and causes the most frequent clinically diagnosed cancer in the animal kingdom. Not only is MDV an important pathogen that threatens the poultry industry but it is also used as a natural virus-host model for herpesvirus-induced tumor formation. In order to visualize MDV-induced lymphoma and to identify potential biomarkers in an unbiased approach, we performed imaging mass spectrometry (IMS) and noncontact laser capture microdissection. This study provides a first description of the visualization of MDV-induced tumors by IMS that could be applied also for diagnostic purposes. In addition, we identified and validated potential biomarkers for MDV-induced tumors that could provide the basis for future research on pathogenesis and tumorigenesis of this malignancy.
Regulation of Cytochrome P450 2B10 (CYP2B10) Expression in Liver by Peroxisome Proliferator-Activated Receptor-β/δ Modulation of SP1 Promoter Occupancy
Takayuki Koga - 2016
Abstract
Alcoholic liver disease is a pathological condition caused by over-consumption of alcohol. Due to the high morbidity and mortality associated with this disease, there remains a need to elucidate the molecular mechanisms underlying its etiology and to develop new treatments. Since peroxisome proliferator-activated receptor-β/δ (PPARβ/δ) modulates ethanol-induced hepatic effects, the present study examined alterations in gene expression that may contribute to this disease. Chronic ethanol treatment causes increased hepatic CYP2B10 expression in Pparβ/δ+/+ mice, but not in Pparβ/δ-/- mice. Nuclear and cytosolic localization of the constitutive androstane receptor (CAR), a transcription factor known to regulate Cyp2b10 expression, was not different between genotypes. Peroxisome proliferator-activated receptor γ co-activator 1α (PGC1α), a co-activator of both CAR and PPARβ/δ, was up-regulated in Pparβ/δ+/+ liver following ethanol exposure, but not in Pparβ/δ-/- liver. Functional mapping of the Cyp2b10 promoter and ChIP assays revealed that PPARβ/δ-dependent modulation of SP1 promoter occupancy up-regulated Cyp2b10 expression in response to ethanol. These results suggest that PPARβ/δ regulates Cyp2b10 expression indirectly by modulating SP1 and PGC1α expression and/or activity independent of CAR activity. Ligand activation of PPARβ/δ attenuates ethanol-induced Cyp2b10 expression in Pparβ/δ+/+ liver, but not in Pparβ/δ-/- liver. Strikingly, Cyp2b10 suppression by ligand activation of PPARβ/δ following ethanol treatment occurred in hepatocytes and was mediated by paracrine signaling from Kupffer cells. Combined, results from the present study demonstrate a novel regulatory role of PPARβ/δ in modulating CYP2B10 that may contribute to the etiology of alcoholic liver disease.
Targeting NF-κB RelA/p65 phosphorylation overcomes RITA resistance
Yiwen Bu - 2016
Abstract
Inactivation of p53 occurs frequently in various cancers. RITA is a promising anticancer small molecule that dissociates p53-MDM2 interaction, reactivates p53 and induces exclusive apoptosis in cancer cells, but acquired RITA resistance remains a major drawback. This study found that the site-differential phosphorylation of nuclear factor-κB (NF-κB) RelA/p65 creates a barcode for RITA chemosensitivity in cancer cells. In naïve MCF7 and HCT116 cells where RITA triggered vast apoptosis, phosphorylation of RelA/p65 increased at Ser536, but decreased at Ser276 and Ser468; oppositely, in RITA-resistant cells, RelA/p65 phosphorylation decreased at Ser536, but increased at Ser276 and Ser468. A phosphomimetic mutation at Ser536 (p65/S536D) or silencing of endogenous RelA/p65 resensitized the RITA-resistant cells to RITA while the phosphomimetic mutant at Ser276 (p65/S276D) led to RITA resistance of naïve cells. In mouse xenografts, intratumoral delivery of the phosphomimetic p65/S536D mutant increased the antitumor activity of RITA. Furthermore, in the RITA-resistant cells ATP-binding cassette transporter ABCC6 was upregulated, and silencing of ABCC6 expression in these cells restored RITA sensitivity. In the naïve cells, ABCC6 delivery led to RITA resistance and blockage of p65/S536D mutant-induced RITA sensitivity. Taken together, these data suggest that the site-differential phosphorylation of RelA/p65 modulates RITA sensitivity in cancer cells, which may provide an avenue to manipulate RITA resistance.

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products