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ToughMix®

PCR inhibitors are common in crude samples and readily compromise assay performance. Use of Quantabio TOUGH-tested ToughMix reagents results in enhanced PCR performance with crude or contaminated samples. The advanced ToughMix buffer technology is engineered to withstand several types of PCR-inhibition, providing robust and reliable results from a variety of starting materials and purification methods.

 

  • Robust fluorescent probe qPCR & PCR performance
  • Applicable to wider range of probe designs and detection chemistries
  • Work directly with crude lysates
  • Bypass exhaustive and expensive sample purification
  • Superior performance in the presence of PCR inhibitors

 

 

Engineered Application Specific Enzymes
Optimized Tough Reagent
Superior Product Performance

 

 

Overcome many common PCR inhibitors:

Inhibitors Common Source
Polysaccharides Plants, seeds, stool
Heme, hemoglobin Blood
Humic acid Soil, plant material
Melanin Skin, hair
Polyphenols Plant extracts

 

 

ToughMix technology is available for different applications:

  Conventional PCR

RT-PCR SYBR-based qPCR Probe-based qPCR RT-qPCR
repliQa HiFi ToughMix AccuStart II PCR ToughMix qScript XLT 1-Step RT-PCR Kit AccuStart Genotyping ToughMix PerfeCTa qPCR ToughMix PerfeCTa Muliplex qPCR ToughMix qScript XLT 1-Step RT-qPCR ToughMix UltraPlex 1-Step ToughMix qScript 1-Step Virus ToughMix
Features

Concentration

2x

2x

2x

2x

2x

5x

2x

4x

2x

Kit Format

1 tube

1 tube mix + 1 tube loading dye

2 tubes

1 tube

1 tube

1 tube

1 tube

1 tube

1 tube

Multiplex Compatibility

Up to 2 targets

Up to 2 targets

Up to 5 targets

Up to 4 targets

Up to 5 targets

Up to 4 targets

Amplicon Length

Up to 24kb

Up to 4kb

Exceeding 4kb RNA

70 – 300 bp

70 – 300 bp

70 – 300 bp

Applications

 

High fidelity PCR, Crude PCR, Fast PCR, Long PCR, High Yield, NGS

Routine PCR, Crude PCR

High fidelity PCR, Crude PCR

SNP genotyping, high yield

Gene expression, inhibitor tolerant, high yield

Gene expression, inhibitor tolerant, high yield

Inhibitor tolerant, high yield

Inhibitor tolerant, high yield

Inhibitor tolerant, high yield

Available Formats

Master Mix

Packaging (Rxns/Units)

25 Rxns
100 Rxns
500 Rxns

100 Rxns
800 Rxns
4000 Rxns

20 Rxns
200 Rxns

1250 Rxns
5000 Rxns

250 Rxns
1250 Rxns
5000 Rxns

250 Rxns
1000 Rxns
5000 Rxns

100 Rxns
500 Rxns
2000 Rxns
5000 Rxns

100 Rxns
500 Rxns
1000 Rxns
10000 Rxns

100 Rxns
500 Rxns
2000 Rxns

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

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