ToughMix PCR Master Mix

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ToughMix^®

PCR inhibitors are common in crude samples and readily compromise assay performance. Use of Quantabio TOUGH-tested ToughMix reagents results in enhanced PCR performance with crude or contaminated samples. The advanced ToughMix buffer technology is engineered to withstand several types of PCR-inhibition, providing robust and reliable results from a variety of starting materials and purification methods.

Robust fluorescent probe qPCR & PCR performance
Applicable to wider range of probe designs and detection chemistries
Work directly with crude lysates
Bypass exhaustive and expensive sample purification
Superior performance in the presence of PCR inhibitors

Engineered Application Specific Enzymes

Optimized Tough Reagent

Superior Product Performance

Overcome many common PCR inhibitors:

Inhibitors	Common Source
Polysaccharides	Plants, seeds, stool
Heme, hemoglobin	Blood
Humic acid	Soil, plant material
Melanin	Skin, hair
Polyphenols	Plant extracts

ToughMix technology is available for different applications:

	Conventional PCR		RT-PCR	SYBR-based qPCR	Probe-based qPCR		RT-qPCR
	repliQa HiFi ToughMix	AccuStart II PCR ToughMix	qScript XLT 1-Step RT-PCR Kit	AccuStart Genotyping ToughMix	PerfeCTa qPCR ToughMix	PerfeCTa Muliplex qPCR ToughMix	qScript XLT 1-Step RT-qPCR ToughMix	UltraPlex 1-Step ToughMix	qScript 1-Step Virus ToughMix
Features
Concentration	2x	2x	2x	2x	2x	5x	2x	4x	2x
Kit Format	1 tube	1 tube mix + 1 tube loading dye	2 tubes	1 tube	1 tube	1 tube	1 tube	1 tube	1 tube
Multiplex Compatibility				Up to 2 targets	Up to 2 targets	Up to 5 targets	Up to 4 targets	Up to 5 targets	Up to 4 targets
Amplicon Length	Up to 24kb	Up to 4kb	Exceeding 4kb RNA				70 – 300 bp	70 – 300 bp	70 – 300 bp
Applications
	High fidelity PCR, Crude PCR, Fast PCR, Long PCR, High Yield, NGS	Routine PCR, Crude PCR	High fidelity PCR, Crude PCR	SNP genotyping, high yield	Gene expression, inhibitor tolerant, high yield	Gene expression, inhibitor tolerant, high yield	Inhibitor tolerant, high yield	Inhibitor tolerant, high yield	Inhibitor tolerant, high yield
Available Formats
Master Mix	•	•		•	•	•	•	•	•
Packaging (Rxns/Units)	25 Rxns 100 Rxns 500 Rxns	100 Rxns 800 Rxns 4000 Rxns	20 Rxns 200 Rxns	1250 Rxns 5000 Rxns	250 Rxns 1250 Rxns 5000 Rxns	250 Rxns 1000 Rxns 5000 Rxns	100 Rxns 500 Rxns 2000 Rxns 5000 Rxns	100 Rxns 500 Rxns 1000 Rxns 10000 Rxns	100 Rxns 500 Rxns 2000 Rxns

ToughMix®

Overcome many common PCR inhibitors:

ToughMix technology is available for different applications:

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

Choose your application from the categories below

Products

ToughMix^®