ToughMix®
PCR inhibitors are common in crude samples and readily compromise assay performance. Use of Quantabio TOUGH-tested ToughMix reagents results in enhanced PCR performance with crude or contaminated samples. The advanced ToughMix buffer technology is engineered to withstand several types of PCR-inhibition, providing robust and reliable results from a variety of starting materials and purification methods.
- Robust fluorescent probe qPCR & PCR performance
- Applicable to wider range of probe designs and detection chemistries
- Work directly with crude lysates
- Bypass exhaustive and expensive sample purification
- Superior performance in the presence of PCR inhibitors
Overcome many common PCR inhibitors:
Inhibitors | Common Source |
Polysaccharides | Plants, seeds, stool |
Heme, hemoglobin | Blood |
Humic acid | Soil, plant material |
Melanin | Skin, hair |
Polyphenols | Plant extracts |
ToughMix technology is available for different applications:
Conventional PCR |
RT-PCR | SYBR-based qPCR | Probe-based qPCR | RT-qPCR | |||||
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repliQa HiFi ToughMix | AccuStart II PCR ToughMix | qScript XLT 1-Step RT-PCR Kit | AccuStart Genotyping ToughMix | PerfeCTa qPCR ToughMix | PerfeCTa Muliplex qPCR ToughMix | qScript XLT 1-Step RT-qPCR ToughMix | UltraPlex 1-Step ToughMix | qScript 1-Step Virus ToughMix | |
Features | |||||||||
Concentration |
2x |
2x |
2x |
2x |
2x |
5x |
2x |
4x |
2x |
Kit Format |
1 tube |
1 tube mix + 1 tube loading dye |
2 tubes |
1 tube |
1 tube |
1 tube |
1 tube |
1 tube |
1 tube |
Multiplex Compatibility |
Up to 2 targets |
Up to 2 targets |
Up to 5 targets |
Up to 4 targets |
Up to 5 targets |
Up to 4 targets |
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Amplicon Length |
Up to 24kb |
Up to 4kb |
Exceeding 4kb RNA |
70 – 300 bp |
70 – 300 bp |
70 – 300 bp |
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Applications |
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High fidelity PCR, Crude PCR, Fast PCR, Long PCR, High Yield, NGS |
Routine PCR, Crude PCR |
High fidelity PCR, Crude PCR |
SNP genotyping, high yield |
Gene expression, inhibitor tolerant, high yield |
Gene expression, inhibitor tolerant, high yield |
Inhibitor tolerant, high yield |
Inhibitor tolerant, high yield |
Inhibitor tolerant, high yield |
Available Formats |
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Master Mix |
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Packaging (Rxns/Units) |
25 Rxns |
100 Rxns |
20 Rxns |
1250 Rxns |
250 Rxns |
250 Rxns |
100 Rxns |
100 Rxns |
100 Rxns |