PerfeCTa PreAmp SuperMix
- Unbiased, linear pre-amplification of up to 100 DNA targets
- 5x concentrated SuperMix maximizes sample input volume with dilute cDNA templates
- Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot-start
- User-friendly inert AccuVue plate loading dye provides visual confirmation of reagent addition
- Supports efficient vortex mixing.
PerfeCTa PreAmp SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Description
PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all the necessary components, except primers and templates. The 5x concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of inert light blue tracer dye helps visualize small reaction volumes and ensure accurate pipetting. PerfeCTa PreAmp SuperMix delivers unbiased pre-amplification of up to 100target sequences from as little as 100
pg of total RNA. It is compatible with both TaqMan® 5’-nuclease probes or dS-DNA binding dye (i.e. SYBR® Green I) qPCR detection chemistries.
Details
5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart™ II hotstart Taq DNA polymerase, inert AccuVue™ plate loading dye, and stabilizers.