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PerfeCTa PreAmp SuperMix

Unbiased pre-amplification of up to 100 DNA targets from as little as 100 pg total cDNA

Features & Benefits

Unbiased, linear pre-amplification of up to 100 DNA targets
5x concentrated SuperMix maximizes sample input volume with dilute cDNA templates
Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot-start
User-friendly inert AccuVue plate loading dye provides visual confirmation of reagent addition
Supports efficient vortex mixing.

PerfeCTa PreAmp SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product

Kit Size

Order Info

PerfeCTa PreAmp SuperMix

Request Sample

40 x 50 μL rxns (1 x 400 µL )

Order (95146-040)

Description

PerfeCTa PreAmp SuperMix is a 5x concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing. It contains all the necessary components, except primers and templates. The 5x concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of inert light blue tracer dye helps visualize small reaction volumes and ensure accurate pipetting. PerfeCTa PreAmp SuperMix delivers unbiased pre-amplification of up to 100target sequences from as little as 100
pg of total RNA. It is compatible with both TaqMan^® 5’-nuclease probes or dS-DNA binding dye (i.e. SYBR^® Green I) qPCR detection chemistries.

Details

Resource Panel

Details

Contents

5X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart™ II hotstart Taq DNA polymerase, inert AccuVue™ plate loading dye, and stabilizers.

Storage & Handling