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qPCR Instrumentation
Real-Time Quantitative PCR
SYBR Green Detection
PerfeCTa SYBR Green SuperMix
PerfeCTa SYBR Green FastMix
Probe-based Detection
PerfeCTa MultiPlex qPCR SuperMix
PerfeCTa qPCR ToughMix
PerfeCTa Multiplex qPCR ToughMix
PerfeCTa FastMix II
PerfeCTa qPCR FastMix UNG
Multiplexed Pre-Amplification
PerfeCTa PreAmp SuperMix
Conventional PCR
AccuStart Taq DNA Polymerase HiFi
AccuStart II PCR SuperMix
AccuStart II Taq DNA Polymerase
AccuStart II PCR ToughMix
5PRIME HotMaster Taq DNA Polymerase
5PRIME HotMasterMix
AccuStart II GelTrack PCR SuperMix
AccuStart Long Range SuperMix
repliQa HiFi ToughMix
repliQa HiFi Assembly Mix
repliQa HiFi ToughMix
Next Generation Sequencing (NGS)
PerfeCTa NGS Quantification Kit
sparQ HiFi PCR Master Mix
sparQ DNA Library Prep Kit
sparQ DNA Frag & Library Prep Kit
sparQ PureMag Beads
sparQ Fast Library Quant Kit (for Q)
Reverse Transcription
Conventional RT-PCR
qScript XLT 1-Step RT-PCR Kit
Quantitative RT-qPCR
qScript XLT 1-Step RT-qPCR ToughMix
qScript One-Step SYBR Green RT-qPCR
UltraPlex 1-Step ToughMix
qScript One-Step RT-qPCR Kit
Qscript lyo 1-step
First-Strand cDNA Synthesis
qScript cDNA SuperMix
qScript XLT cDNA SuperMix
qScript cDNA Synthesis Kit
qScript Flex cDNA Kit
PerfeCTa DNase I
Sample Preparation
Extracta DNA Prep
5PRIME Phase Lock Gel
Extracta DBS
AccuStart II PCR Genotyping Kit
AccuStart Genotyping ToughMix
AccuMelt HRM SuperMix
microRNA Profiling
qScript microRNA cDNA Synthesis Kit
PerfeCTa SYBR Green SuperMix
Product Manuals
Safety Data Sheets (SDS)
CofA (PSF)
Product Flyers
Technical Notes
Customer Profile Stories
  • Next Generation Sequencing (NGS)
    How many libraries can I run at one time?
    A run on a single Q qPCR cycler can accommodate 48 reactions. We recommend setting up triplicate reactions for each library or standard. The default template files are preconfigured with information about the six sparQ DNA standards included as triplicate reactions in the run. This setup allows room for triplicate reactions of 10 unknown libraries per run. At least two serial dilutions of each library are recommended to ensure that diluted libraries fall within the linear dynamic range of the standards. If the workflows are established, the number of the replicates or the dilutions may be reduced in order to increase throughput.
    What if I don’t want to use the default template files?
    Array and run files can be manually defined to give more flexibility regarding the number of replicates, the position of the DNA standards, or the cycling paraments. Please consult the step-by-step technical guide for setting up sparQ Fast Library Quant run files without a template (available under Related Resources).
    How do I use the default template files for assay set up?
    Template files provide the Q software information about assay and run parameters. These are pre-configured with the cycling protocol and the information about locations of the six sparQ DNA standards included as triplicate reactions in the run. Use the 20ul.QTemplate if reactions are 20 μl volume and use the 10ul.QTemplae if reactions are 10 ul volume. Please ensure templates files are stored in the appropriate folder location (My Documents > Quantabio > Q-qPCR > Templates) and use the Q-qPCR software to open the template files.
    What are the storage recommendations for the sparQ Fast Library Quant Kit?
    Store kit components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt. When the sparQ Fast Library Quant Kit is appropriately stored and handled, the expiration date is 18 months from the date of manufacture. For repeated, short-term use, all components of the kit can be kept at 2-8C for up to 4 weeks.
    What are the primer sequences used in the sparQ Fast Mastermix?
    Primer sequences correspond to the Illumina P5 and P7 sequences. Forward primer: 5’-AATGATACGGCGACCACCGA-3’ Reverse primer: 5’-CAAGCAGAAGACGGCATACGA-3’
    What is the size and concentration of the DNA standards included in the kit?
    The sparQ Fast Library Quant Kit contains a set of six, pre-diluted DNA standards ranging from 0.0002 pM to 20 pM (a 10-fold dilution series). The standards are linear, dsDNA of 426 bp.
    Can I use the sparQ Fast Library Quant Kit on my own qPCR instrument?
    The sparQ Fast Library Quant Kit is specifically developed and optimized for use with the Q qPCR instrument, which supports fast cycling protocols with 40 minutes or less run time.
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