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Next Generation Sequencing (NGS)How many libraries can I run at one time?A run on a single Q qPCR cycler can accommodate 48 reactions. We recommend setting up triplicate reactions for each library or standard. The default template files are preconfigured with information about the six sparQ DNA standards included as triplicate reactions in the run. This setup allows room for triplicate reactions of 10 unknown libraries per run. At least two serial dilutions of each library are recommended to ensure that diluted libraries fall within the linear dynamic range of the standards. If the workflows are established, the number of the replicates or the dilutions may be reduced in order to increase throughput.What if I don’t want to use the default template files?Array and run files can be manually defined to give more flexibility regarding the number of replicates, the position of the DNA standards, or the cycling paraments. Please consult the step-by-step technical guide for setting up sparQ Fast Library Quant run files without a template (available under Related Resources).How do I use the default template files for assay set up?Template files provide the Q software information about assay and run parameters. These are pre-configured with the cycling protocol and the information about locations of the six sparQ DNA standards included as triplicate reactions in the run. Use the 20ul.QTemplate if reactions are 20 μl volume and use the 10ul.QTemplae if reactions are 10 ul volume. Please ensure templates files are stored in the appropriate folder location (My Documents > Quantabio > Q-qPCR > Templates) and use the Q-qPCR software to open the template files.What are the storage recommendations for the sparQ Fast Library Quant Kit?Store kit components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt. When the sparQ Fast Library Quant Kit is appropriately stored and handled, the expiration date is 18 months from the date of manufacture. For repeated, short-term use, all components of the kit can be kept at 2-8C for up to 4 weeks.What are the primer sequences used in the sparQ Fast Mastermix?Primer sequences correspond to the Illumina P5 and P7 sequences. Forward primer: 5’-AATGATACGGCGACCACCGA-3’ Reverse primer: 5’-CAAGCAGAAGACGGCATACGA-3’What is the size and concentration of the DNA standards included in the kit?The sparQ Fast Library Quant Kit contains a set of six, pre-diluted DNA standards ranging from 0.0002 pM to 20 pM (a 10-fold dilution series). The standards are linear, dsDNA of 426 bp.Can I use the sparQ Fast Library Quant Kit on my own qPCR instrument?The sparQ Fast Library Quant Kit is specifically developed and optimized for use with the Q qPCR instrument, which supports fast cycling protocols with 40 minutes or less run time.