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Sample PreparationWhat is the shelf life of the Extracta DBS product?The shelf life is 2 years stored at 2-8°C.What is the Extracta DBS kit and what are some of the advantages of using this kit?The Extracta DBS kit is a research use only reagent for rapid isolation of DNA from dried blood spots. It is supplied as a convenient “ready to use” solution that reduces sample preparation time and is compatible with automation. The numerous wash and/or proteolytic digestion steps of other protocols are not necessary with Extracta DBS and the eluted DNA solution does not interfere with downstream DNA amplification applications. Qiagen Beverly’s ISO13485 manufacturing process ensures consistent composition and performance across lots.Should all of the supernatant be removed after the first wash and centrifugation step?After the first wash and centrifugation step, remove and discard as much liquid and debris as possible. The DNA remains on the filter paper at this point.What Quantabio products have confirmed compatibility with DNA extracted by the Extracta DBS kit?The following Quantabio products have been demonstrated to perform well with the extracted DNA: Accustart Genotyping ToughMix (95115, 95116, 95117) PerfeCTa Multiplex qPCR ToughMix (95147, 95148, 95149) PerfeCTa qPCR ToughMix (95112, 95113, 95114, 95138, 95139, 95140)For what downstream applications can the extracted DNA be used?The extracted DNA is compatible with a variety of end-point PCR, real-time PCR, next generation sequencing, or Sanger sequencing procedures.Can the extracted DNA be stored?We recommend using the DNA in downstream applications immediately after extraction. If this is not possible, the extracted DNA can be refrigerated at 2-8°C for short term storage (1-2 days) or stored frozen at -15 to -25°C for prolonged storage. We have no data on the stability of extracted DNA after prolonged storage.What is the stability of the extracted DNA?We have no data on the stability of the extracted DNA. We recommend that the DNA be used for downstream applications as soon as possible following extraction.How should the extracted DNA be measured?Measurement of DNA by methods using DNA binding dyes or absorbance at 260 nm are not applicable to a crude lysis method such as the Extracta DBS method. The recommended way to accurately quantify DNA in an Extracta DBS lysate is to measure a specific DNA sequence (generally a single-copy gene in gDNA) by qPCR.