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PCR & qPCR ToughMixes Engineered For Difficult Samples
ToughMixes neutralize problem-causing inhibitors present in crude samples, avoiding expensive and time-consuming purification steps.
Blood Sample
Blood

Avoid DNA polymerase, nucleic acid, and other common PCR inhibitors in crude blood samples with Quantabio ToughMixes.

Dried Blood Spots

Overcome the limitations of sensitivity and specificity in dried blood samples with Quantabio’s advanced ToughMixes.

Plant & Soil

Our ToughMix reagents withstand common PCR-suppressive compounds in plant & soil samples, such as phenols, polysaccharides, and humic acid.

sparQ Sequencing Solutions

Improve Performance
& Reduce Costs

Quantabio offers complete library prep solutions for Illumina NGS platforms. High-quality reagents deliver unmatched efficiency and robust performance to ensure reliable and reproducible sequencing results while reducing total costs.

Compatible Workflows
Whole genome
Whole transcriptome
Target capture
Amplicon
ChIP
Key Benefits
Higher yields
Better coverage
Greater uniformity
Easier workflows
Lower total costs
Library_prep

Library Preparation

HiFi Amplification

Purification &
Size Selection
Library Quantification
Customer Testimonials
Q qPCR Instrument
My group was looking for a qPCR machine that didn’t take up much bench space or require annual maintenance, which the Q machine fits. Additional features I like include its ability to multiplex with up to 4 dyes in the same tube, its relative ease of use, and free software that can be used on anyone’s PC making it easy for everyone in the group to use. We’ve just begun using the machine, but so far I’ve been happy with the data acquisition obtained from it.
Max Rogers | Associate Director, Pathology and Toxicology Sciences
UltraPlex 1-Step ToughMix

"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."

Rhoel R. D., PhD, MPH Professor of Infectious Diseases UF Eminent Scholar Director | CDC Southeastern Regional Center of Excellence for Vector Borne Diseases
qScript 1-Step Virus ToughMix

"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."

Guillermo C. | Project Coordinator Sick Kids Molecular Diagnostics Lab
repliQa HiFi ToughMix

"The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."

Simon S. | Professor, Tennessee State University
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center
LAMP Assay on Q

"Performing a LAMP assay using a Q-qPCR machine is really comparable with Genie II, however, the higher number of samples that can be analysed at a time and also the appearance of the first amplification signals 2 min earlier than those in Genie II, adds up to its value!"

Steve Baeyen | Flanders Research Institute for agriculture, fisheries and food
PerfeCTa SYBR Green SuperMix

"PerfeCTa SYBR Green SuperMix has been my first and successful experience that boosted my confidence for qPCR. I am really satisfied with its performance."

Teaching Assistant | Ohio University
Q qPCR Instrument

“We had the pleasure of testing the ToughMix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence. The ToughMix stood up to it’s name and had beautiful Ct’s….Considering it’s size, intuititive software and near reproducible Ct curves we’ve decided to add the Q as our workhorse.”

Assistant Professor | Infectious Diseases Global and Immigrant Health, Baylor College of Medicine
UltraPlex 1-Step ToughMix

"In side-by-side comparison, QuantaBio UltraPlex 1-Step ToughMix out performed ABI TaqPath, ABI TaqMan FastVirus, and ABI AgPath-ID One-Step RT-PCR kits."

Sladana B. | Plant Disease Diagnostician
sparQ PureMag Beads

“I used the sparQ PureMag beads for mRNA purification after in vitro transcription. The sparQ beads demonstrated an excellent recovery (~98%) and could be used for size selection of the desired mRNA product. Highly recommended."

Postdoctoral Fellow | Dana Farber Cancer Institute
repliQa HiFi ToughMix

"This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."

Lili C. | Post Doc, USC
qScript XLT 1-Step RT-qPCR ToughMix

"It is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."

Olivia L. | Senior Research Engineer
PerfeCTa MultiPlex qPCR ToughMix

"The Perfecta Multiplex qPCR ToughMix greatly simplified my qPCR assay by removing DNA isolation steps and complexity of passive reference dyes. I will definitely be using this product in the future."

Bethany B. | Biotech company
qScript XLT cDNA SuperMix

"qScript XLT gave us a very clean cDNA that upon qPCR allowed a detection of the gene expression 3-8 cycles earlier than in our current RT."

Ernesto D. | UCSF
Extracta DBS

“The simple and elegant way to unlock genetic information”

Mei Baker, MD, FACMG | Co-Director of Newborn Screening - Wisconsin
PerfeCTA SYBR Green FastMix

"With the PerfeCTa SYBR Green FastMix we had less variability in our triplicates and more reproducible results between repeat experiments."

With the PerfeCTa SYBR Green FastMix we had less variability in our triplicates and more reproducible results between repeat experiments. | Professor, UCSF

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

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