UltraPlex 1-Step ToughMix
- 4X concentrated master mix allows greater flexibility with dilute samples – as little as 1 pg of total RNA
- Multiplexing capable with up to 5 targets
- Tough-tested for applications where PCR inhibition from template impurities may be a concern, such as: crude extracts, clinical specimens, or environmental samples.
- Temperature stabilized master mix enables convenient reaction setup at ambient room temperature
UltraPlex 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
UltraPlex 1-Step ToughMix
UltraPlex 1-Step ToughMix ROX
UltraPlex 1-Step ToughMix Low ROX
Description
UltraPlex 1-Step ToughMix is a ready-to-use, 4X concentrated master mix 1-step reverse transcription and real-time quantitative PCR (RT-qPCR) of RNA templates using probe-based detection methods. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between steps. Optimized to deliver highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or highly multiplexed RNA detection assays, this reagent chemistry is optimized to deliver maximum assay sensitivity, precision and reproducibility with miniaturized reaction volumes and either conventional or accelerated thermal cycling protocols. UltraPlex 1-Step ToughMix contains all required components for RT-qPCR except RNA template and probe and is compatible with all dual-labeled probe chemistries.
Details
UltraPlex 1-Step ToughMix (Low ROX/ ROX) (4X), contains:
- 4X reaction buffer containing optimized titrations of qPCR-grade dATP, dCTP, dGTP, dTTP, and magnesium.
- Ultra-pure recombinant proteins: qScript XLT reverse transcriptase, RNase inhibitor protein, and AccuStart II hot-start Taq DNA polymerase.
- Proprietary stabilizers additives and optimized ROX passive reference dye (if applicable).
Instrument Compatibility
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Analytik Jena qTower
- Analytik Jena qTOWERiris
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Resources
Flyers
Product Manuals
Application Notes
CofA (PSFs)
SDSs
Publications
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Customer Product Reviews
| 5 star | 80% | |
| 4 star | 20% | |
| 3 star | 0% | |
| 2 star | 0% | |
| 1 star | 0% |
UltraPlex 1-Step ToughMix
For this mix it helped us, to use alot less primer and probe than with our current kit, and maybe it’s due to our current kit not being optimized. But saving on primers and probe can be very helpful. It was able to be used without adapting our current methods, to the requirements of the kit. So it has worked well, and can definitely be an option for us to use in the future. As well as it use for both multi- and single-plex assays.
We set up a very challenging RT-PCR reaction and the master mix handled it very well. The 4X formulation allowed us to work with increased input volume, effective improved sensitivity. It’s a great product!
We compared the UltraPlex 1-Step ToughMix (4X) 100R to our legacy enzyme for influenza testing. The UltraPlex performed very well on all samples tested with Ct values slightly lower than the legacy enzyme.
I compared the UltraPlex 1-Step ToughMix (4x) L-ROC against my current mastermix (TaqMan Fast-Virus 1-step mastermix), and was impressed with the performance of the UltraPlex mastermix relative to the Fast-virus mastermix. I saw reduced background noise and lower Ct scores in the 30-40 Cycle range. In addition, I did not modify my current protocol so there is a possibility that with some optimization, the differences could be even greater. Thanks for sending the sample. I will be using this mastermix in the future.