ToughMix®
- Robust fluorescent probe qPCR & PCR performance
- Applicable to wider range of probe designs and detection chemistries
- Work directly with crude lysates
- Bypass exhaustive and expensive sample purification
- Superior performance in the presence of PCR inhibitors
Overcome many common PCR inhibitors:
| Inhibitors | Common Source |
|---|---|
| Polysaccharides | Plants, seeds, stool |
| Heme, hemoglobin | Blood |
| Humic acid | Soil, plant material |
| Melanin | Skin, hair |
| Polyphenols | Plant extracts |
ToughMix technology is available for different applications:
| Conventional PCR | Genotyping | qPCR | RT-qPCR | |||
|---|---|---|---|---|---|---|
| AccuStart II PCR ToughMix | qScript XLT 1-Step RT-PCR Kit | AccuStart Genotyping ToughMix | PerfeCTa qPCR ToughMix | PerfeCTa Muliplex qPCR ToughMix | qScript XLT 1-Step RT-qPCR ToughMix | UltraPlex 1-Step ToughMix |
| 2X PCR SuperMix | High fidelity cDNA | 2X master mix | 2X master mix | 5X master mix | 2X master mix | 4X master mix |
| Ultrapure hotstart Polymerase | 1 tube SuperMix | SNP detection | anti-foaming technology | Multiplexing up to 5 targets | 1 tube SuperMix | 1 tube SuperMix |
| GelTrack, loading dye (separate tube) | Up to 2 kb | anti-foaming technology | AccuVue for plate loading control | anti-foaming technology | Up to 3 targets | Up to 5 targets |
| GelTrack, loading dye (separate tube) | AccuVue for plate loading control | fast or standard qPCR cycling conditions | fast or standard qPCR cycling conditions | |||
| fast or standard qPCR cycling conditions | Starting from 1 pg of total RNA. | |||||
ToughMix® Products
-
qScript XLT 1-Step RT-qPCR ToughMix
A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods -
UltraPlex 1-Step ToughMix
An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials -
AccuStart Genotyping ToughMix
An advanced 2X concentrated ready-to-use reaction cocktail optimized with destabilizing buffer chemistry to enable accurate SNP genotyping with crude extracts, clinical specimens, environmental samples, plant tissues, animal tissues, or complex food matrices -
PerfeCTa Multiplex qPCR ToughMix
An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods