• Robust fluorescent probe qPCR & PCR performance
  • Applicable to wider range of probe designs and detection chemistries
  • Work directly with crude lysates
  • Bypass exhaustive and expensive sample purification
  • Superior performance in the presence of PCR inhibitors


Engineered Application Specific Enzymes
Optimized Tough Reagent
Superior Product Performance


Overcome many common PCR inhibitors:

InhibitorsCommon Source
Polysaccharides Plants, seeds, stool
Heme, hemoglobin Blood
Humic acid Soil, plant material
Melanin Skin, hair
Polyphenols Plant extracts

ToughMix technology is available for different applications:

Conventional PCRGenotypingqPCRRT-qPCR
AccuStart II PCR ToughMix qScript XLT 1-Step RT-PCR Kit AccuStart Genotyping ToughMix PerfeCTa qPCR ToughMix PerfeCTa Muliplex qPCR ToughMix qScript XLT 1-Step RT-qPCR ToughMix UltraPlex 1-Step ToughMix
2X PCR SuperMix High fidelity cDNA 2X master mix 2X master mix 5X master mix 2X master mix 4X master mix
Ultrapure hotstart Polymerase 1 tube SuperMix SNP detection anti-foaming technology Multiplexing up to 5 targets 1 tube SuperMix 1 tube SuperMix
GelTrack, loading dye (separate tube) Up to 2 kb anti-foaming technology AccuVue for plate loading control anti-foaming technology Up to 3 targets Up to 5 targets
GelTrack, loading dye (separate tube) AccuVue for plate loading control fast or standard qPCR cycling conditions fast or standard qPCR cycling conditions
fast or standard qPCR cycling conditions Starting from 1 pg of total RNA.

ToughMix® Products

  • qScript XLT 1-Step RT-qPCR ToughMix
    A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods
  • UltraPlex 1-Step ToughMix
    An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials
  • AccuStart Genotyping ToughMix
    An advanced 2X concentrated ready-to-use reaction cocktail optimized with destabilizing buffer chemistry to enable accurate SNP genotyping with crude extracts, clinical specimens, environmental samples, plant tissues, animal tissues, or complex food matrices
  • PerfeCTa Multiplex qPCR ToughMix
    An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods