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Extracta DBS

PCR-ready genomic DNA from dried blood spots
Features & Benefits
  • Optimized for DNA extraction from dried blood spot punches
  • Single reagent for PCR-ready DNA in 30 minutes
  • Maximized assay sensitivity, lower Cq values, when combined with Quantabio ToughMix®
  • Compatible with high-throughput automation for PCR, qPCR and NGS applications

 

Extracta DBS is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Extracta DBS

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Kit Size:  1 x 10 mL
Part Number:  95171-010
Price:  $50.00
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Kit Size:  1 x 500 mL
Part Number:  95171-500
Price:  $636.00
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Description

Extracta DBS is a ready-to-use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman 903 filter paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing (1-3) reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix or PerfeCTa MultiPlex qPCR ToughMix enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

Combine with ToughMix and maximize qPCR assay sensitivity

Extracta DBS is the perfect match with Quantabio ToughMix for sensitive and precise target quantification. The crude extraction combined with ToughMix, a Quantabio master mix that is tolerant to common PCR inhibitors, results in higher DNA yields independent of DNA sample inputs and qualities to enable accurate detection and high sensitivity even with low copy targets.

Reliable, Consistent Lot-to-Lot Performance

Low amounts and quality of DNA recovered from dried blood spots commonly restrict the utilization of DNA. To overcome these limitations, Extracta DBS increases the yield and quality allowing for efficient and reliable recovery of DNA from dried blood spots. Manufactured under stringent quality standards, Extracta DBS ensures uniform lot-to-lot performance resulting in reliable reproducibility in combination with Quantabio ToughMix.

Featured Publication

A Multiplexed Real-time PCR Assay to Detect SCID and SMN1 Homozygous Exon 7 Deletion, and A Droplet Digital PCR Assay to Assess SMN2 Copy Numbers, in Newborn screening for Spinal Muscular Atrophy

Download Featured Poster

 

What Customers Say

The simple and elegant way to unlock genetic information

Customer Profile Story

Co-Director of Newborn Screening
Mei Baker, MD, FACMG
Details

Details

Storage & Handling

Extracta DBS is stable for up to 2 years at 2-8°C. Please consult the product box label for expiration date information.

Customer Testimonials

Customer Testimonials

Extracta DBS
"Compared to the traditional column based extraction, Extracta DBS is superb and unrivaled."
Fellow | University College London
Extracta DBS

“The simple and elegant way to unlock genetic information”

Mei Baker, MD, FACMG | Co-Director of Newborn Screening - Wisconsin

Details

Storage & Handling

Extracta DBS is stable for up to 2 years at 2-8°C. Please consult the product box label for expiration date information.

Performance Data

Resources

Customer Profile Stories

Product Manuals

Application Notes

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

FAQs

What is the shelf life of the Extracta DBS product?
The shelf life is 2 years stored at 2-8°C.
What is the Extracta DBS kit and what are some of the advantages of using this kit?
The Extracta DBS kit is a research use only reagent for rapid isolation of DNA from dried blood spots. It is supplied as a convenient “ready to use” solution that reduces sample preparation time and is compatible with automation. The numerous wash and/or proteolytic digestion steps of other protocols are not necessary with Extracta DBS and the eluted DNA solution does not interfere with downstream DNA amplification applications. Qiagen Beverly’s ISO13485 manufacturing process ensures consistent composition and performance across lots.
Should all of the supernatant be removed after the first wash and centrifugation step?
After the first wash and centrifugation step, remove and discard as much liquid and debris as possible. The DNA remains on the filter paper at this point.
What Quantabio products have confirmed compatibility with DNA extracted by the Extracta DBS kit?
The following Quantabio products have been demonstrated to perform well with the extracted DNA: Accustart Genotyping ToughMix (95115, 95116, 95117) PerfeCTa Multiplex qPCR ToughMix (95147, 95148, 95149) PerfeCTa qPCR ToughMix (95112, 95113, 95114, 95138, 95139, 95140)
For what downstream applications can the extracted DNA be used?
The extracted DNA is compatible with a variety of end-point PCR, real-time PCR, next generation sequencing, or Sanger sequencing procedures.
Can the extracted DNA be stored?
We recommend using the DNA in downstream applications immediately after extraction. If this is not possible, the extracted DNA can be refrigerated at 2-8°C for short term storage (1-2 days) or stored frozen at -15 to -25°C for prolonged storage. We have no data on the stability of extracted DNA after prolonged storage.
What is the stability of the extracted DNA?
We have no data on the stability of the extracted DNA. We recommend that the DNA be used for downstream applications as soon as possible following extraction.
How should the extracted DNA be measured?
Measurement of DNA by methods using DNA binding dyes or absorbance at 260 nm are not applicable to a crude lysis method such as the Extracta DBS method. The recommended way to accurately quantify DNA in an Extracta DBS lysate is to measure a specific DNA sequence (generally a single-copy gene in gDNA) by qPCR.

Publications

Customer Testimonials

Extracta DBS
"Compared to the traditional column based extraction, Extracta DBS is superb and unrivaled."
Fellow | University College London
Extracta DBS

“The simple and elegant way to unlock genetic information”

Mei Baker, MD, FACMG | Co-Director of Newborn Screening - Wisconsin

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