Real-Time qPCR Reagents

PerfeCTa qPCR reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR.  Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs.

PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.

Quantabio quantitative real-time PCR reagents are optimized to work in series with high performance qScript RT reagents (for RNA targets) and Extracta DNA Prep (for DNA targets).

qPCR comparison chart

  SYBR Green Detection Probe Based Detection
  PerfeCTa SYBR Green SuperMix PerfeCTa SYBR Green FastMix AccuStart Genotyping ToughMix PerfeCTa qPCR FastMix UNG PerfeCTa
FastMix II
PerfeCTa qPCR ToughMix PerfeCTa qPCR ToughMix, UNG PerfeCTa Multiplex qPCR ToughMix PerfeCTa Multiplex qPCR SuperMix
Features
Concentration 2x 2x 2x 2x 2x 2x 2x 5x 2x
Sample Type gDNA, cDNA gDNA, cDNA gDNA, cDNA gDNA, cDNA gDNA, cDNA gDNA, cDNA gDNA, cDNA gDNA, cDNA gDNA, cDNA
Fast Cycling No Yes Yes Yes Yes Yes Yes Yes Yes
Multiplex Compatibility N/A N/A Up to 2 targets Up to 2 targets Up to 2 targets Up to 2 targets Up to 2 targets Up to 5 targets Up to 4 targets
Carryover Contamination Control N/A N/A N/A Heat-labile UNG & blend of dTTP/dUTP N/A N/A Heat-labile UNG & blend of dTTP/dUTP N/A N/A
Applications
Gene Expression
microRNA
ChIP
SNP Genotyping
Inhibitor Tolerant **
High Yield **
Available Formats
Master Mix
Packaging (Rxns/Units) 250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1250 Rxns
5000 Rxns
250 Rxns
1000 Rxns
5000 Rxns
50 Rxns
200 Rxns
1000 Rxns

**Recommended product

Featured Products

  • PerfeCTa SYBR Green SuperMix
    User-friendly, stabilized 2X concentrated reaction cocktail optimized to produce maximum optical signal from especially small qPCR amplicons. Not recommended for fast thermal cycling conditions (anneal/elongation step <30 seconds)

Multiplexed Pre-Amplification

  • PerfeCTa PreAmp SuperMix
    5X concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing.

SYBR Green Detection

  • PerfeCTa SYBR Green FastMix
    User-friendly, stabilized 2X concentrated reaction cocktail with proprietary AccuFast DNA polymerase technology for highly sensitive and specific SYBR Green based detection with either fast or standard thermal cycling conditions
  • PerfeCTa SYBR Green SuperMix
    User-friendly, stabilized 2X concentrated reaction cocktail optimized to produce maximum optical signal from especially small qPCR amplicons. Not recommended for fast thermal cycling conditions (anneal/elongation step <30 seconds)

Probe-based Detection

  • PerfeCTa Multiplex qPCR ToughMix
    An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods
  • PerfeCTa qPCR ToughMix
    A 2X concentrated ready-to-use reaction cocktail for quantitative DNA detection with crude extracts or inhibitory starting materials such as clinical or environmental specimens, plant tissues, animal tissues, and complex food matrices
  • PerfeCTa qPCR FastMix UNG
    Fast qPCR cycling that eliminates carry-over contamination
  • PerfeCTa FastMix II
    An advanced 2X concentrated qPCR SuperMix reagent optimized to support both fast and standard thermal cycling conditions with probe-based real-time quantitative PCR assays
  • PerfeCTa MultiPlex qPCR SuperMix
    A 2X concentrated, ready-to-use reaction cocktail containing all the necessary components for highly-multiplexed, real-time quantitative PCR - except primers, probe(s), and DNA template