Specific & Efficient Primer Extension
Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity. That’s why Quantabio PCR reagents are formulated with ultrapure AccuStart™ DNA polymerase, which contains a stringent antibody hot start to ensure specific and efficient primer extension only after activation at 94°C.
Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.
Quantabio manufactured reagents:
- Maximize assay performance
- Minimize errors
- Deliver reliable, reproducible results
Featured Products
PCR Comparisons
AccuStart II PCR SuperMix | AccuStart II GelTrack PCR SuperMix | sparQ HiFi PCR Master Mix | AccuStart II PCR ToughMix | repliQa HiFi ToughMix | AccuStart Long Range SuperMix | |
---|---|---|---|---|---|---|
Features | ||||||
Concentration | 2x | 2x | 2x | 2x | 2x | 4x |
Amplicon Size | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 24 kb | Up to 24 kb |
Extension Time* | 60 sec/kb | 60 sec/kb | 30 sec/kb | 60 sec/kb | 1-10 sec/kb | 30-60 sec/kb |
Multiplex PCR | Up to 6 Targets | |||||
Fidelity vs. Taq | 1x | 1x | 80x Taq | 1x | 90x Taq | 10-12x Taq |
dU Tolerant | No | No | No | No | Yes | Yes |
5′ – 3′ exo | x | x | N/A | x | N/A | N/A |
3′ – 5′ exo | N/A | N/A | x | N/A | x | x |
Resulting Ends | 3′ dA overhang | 3′ dA overhang | Blunt | 3′ dA overhang | Blunt | Blunt/T overhangs |
Applications | ||||||
Routine PCR | x | x | x | x | x | |
High Fidelity | x | ** | x | |||
Crude PCR | x | ** | ||||
Fast PCR | ** | |||||
Long PCR | x | ** | ||||
High Yield | x | x | ** | x | ||
NGS Library Amplification | ** | x | ||||
Available Formats | ||||||
Hot Start | x | x | x | x | x | x |
Master Mix | x | x | x | x | x | x |
Loading Dye Available | x | x | ||||
Packaging (Rxns/Units) | 100 Rxns | 100 Rxns | 50 Rxns | 100 Rxns | 25 Rxns | 25 Rxns |
qPCR Comparisons
PerfeCTa SYBR Green SuperMix | PerfeCTA SYBR Green FastMix | AccuStart Genotyping ToughMix | PerfeCTa qPCR FastMix UNG | PerfeCTa FastMix II | PerfeCTa qPCR ToughMix | PerfeCTa qPCR ToughMix, UNG | PerfeCTa Multiplex qPCR ToughMix | PerfeCTa Multiplex qPCR SuperMix | |
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Features | |||||||||
Concentration | 2x | 2x | 2x | 2x | 2x | 2x | 2x | 5x | 2x |
Sample Type | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA |
Fast Cycling | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
Multiplex Compatibility | N/A | N/A | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 5 targets | Up to 4 targets |
Carryover Contamination Control | N/A | N/A | N/A | Heat-labile UNG & blend of dTTP/dUTP | N/A | N/A | Heat-labile UNG & blend of dTTP/dUTP | N/A | N/A |
Applications | |||||||||
Gene Expression | x | x | x | x | x | x | x | x | |
microRNA | x | x | |||||||
ChIP | x | x | |||||||
SNP Genotyping | x | ||||||||
Inhibitor Tolerant | x | ** | x | x | |||||
High Yield | x | x | x | ** | |||||
Available Formats | |||||||||
Master Mix | x | x | x | x | x | x | x | x | x |
Packaging (Rxns/units) | 250 Rxns | 250 Rxns | 250 Rxns | 250 Rxns | 250 Rxns | 250 Rxns | 250 Rxns | 250 Rxns | 50 Rxns |
Find the additional information you need to learn more about our PCR & qPCR solutions.
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