AccuStart Genotyping ToughMix

enables probe-based genetic analysis (SNP detection and allelic discrimination) directly from crude extracts, DBS punches, plant tissue and clinical specimens

Features & Benefits

  • Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and improved cluster separation for critical, single-nucleotide polymorphism (SNP) detection assays
  • Sensitive, precise detection with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
  • ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
  • Easy-to-use 2x concentrated SuperMix with AccuVue plate loading dye and pre-blended passive reference dye simplifies reaction setup
  • Supports efficient vortex mixing with proprietary anti-foaming technology


Genotyping ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.


Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. This proprietary formulation has been rigorously optimized to destabilize single base-pair mismatches to ensure precise allelic discrimination and cluster separation with SNP detection assays. The reagent is provided as a 2x concentrated readyto- use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. Inert AccuVue plate loading dye helps to minimize pipette error and provides visual confirmation of thorough mixing.

Performance Data

ToughMix vs Competitor

Comparison to conventional master mixes

AccuStart Genotyping ToughMix stands up to the challenge where other genotyping master mixes fall apart. ToughMix can be used with clean templates where it generates higher fluorescent signal and tighter clusters than the leading competitors mix.


Influence of PCR inhibitor

Comparison to conventional master mixes

In the presence of a common PCR inhibitor, humic acid (50ng/l), the competitors system is completely shut down while ToughMix delivers robust, accurate results



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