perfeCTa qPCR ToughMix

Exceptional Stability for Tough-Tested real-time PCR

Features & Benefits

ToughMix additives neutralize PCR inhibitors to ensure reliable assay performance with a spectrum of starting materials including: clinical specimens, plants, soil, environmental or complex food matrices
Superior assay sensitivity and target precision with AccuStart™ II enzyme technology – maximum-yielding, engineered Taq DNA polymerase with stringent, ultrapure antibody hotstart
Easy-to-use 2X concentrated master mixes with AccuVue™ plate loading dye and optimized passive reference dye for simplified reaction setup
Supports efficient vortex mixing with proprietary anti-foaming technology
Consistent and reliable performance with exceptional reagent stability (uninhibited after 20X freeze-thaw or 30 days at 22°C)

PerfeCTa qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Instrument compatibility

PerfeCTa qPCR ToughMix – ROX

Request Sample

ROX

Applied Biosystems 5700
Applied Biosystems 7300
Applied Biosystems 7900
Applied Biosystems 7900 HT Fast
Applied Biosystems StepOnePlus™

PerfeCTa qPCR ToughMix Low-ROX

Request Sample

Low ROX

Applied Biosystems 7500
Applied Biosystems 7500 Fast
Stratagene Mx3000P®
Stratagene Mx3005P™
Stratagene Mx4000™
Applied Biosystems ViiA 7
Applied Biosystems QuantStudio™
Agilent AriaMx
Douglas Scientific IntelliQube®

PerfeCTa qPCR ToughMix

Request Sample

No ROX

Quantabio Q
BioRad CFX
Roche LightCycler 480
QIAGEN Rotor-Gene Q
Other

PerfeCTa qPCR ToughMix is available in formulations containing UNG (uracil-N-glycosylase). To learn more or request a sample, visit the product page

What Scientists are Saying

PerfeCTa qPCR ToughMix

"Higher sensitivity, positives at a lower Cq. Great results with plant DNA with high inhibition level."

Paula S. | Lab Coordinator INIAV

Q RT-PCR machine in Mbabane Swaziland

We had the pleasure of testing out the ToughMix Mastermix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence.” As it’s name implies, the ToughMix stood up to its name and made the 38 hour trip from Houston to Mbabane, Swaziland and had beautiful Ct curves. Similarly, the Q PCR machine was an ease to travel with as carry on luggage. It’s 25 minute run gave Ct curves identical to our existing, and much bulkier, RT-PCR machine that was already on site in Swaziland. Considering it’s size, intuitive software, and near perfect reproducible Ct curves, we’ve decided to add the Q as our workhorse RT-PCR machine

Assistant Professor | Infectious Diseases Global and Immigrant Health Baylor College of Medicine

PerfeCTa qPCR ToughMix

"This eliminates a costly and time consuming step to remove inhibitors manually, so it has saved us money on reagents and time for our small lab."

Max O. | Conservationist