Extracta DNA Prep

Features & Benefits

• Simple, reagent-based system requires minimal technical skill
• Incubation step can be carried out in 96-well PCR plates or tubes using a standard DNA thermal cycler
• Compatible with a wide-range of clinical specimens, plant and animal tissues, and environmental samples
• Optional stabilization buffer allows for extended storage of extracted DNA templates

 

Extracta DNA Prep is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Extracta DNA Prep for PCR is a two-component reagent kit for rapid extraction of PCR-ready genomic DNA from a variety of tissues. Samples are processed in less than 30 minutes with minimal hands-on time and technical skill. Extracted genomic DNA is suitable for sensitive downstream PCR applications including end-point PCR, High Resolution Melt Analysis (HRM) and quantitative real-time PCR (qPCR) without requiring any additional clean-up. In addition, the extracted DNA may be used in multiplexed PCR applications such as transgene or knock-out analyses. Tissue extractions can be done in tubes, plates or deep-well blocks to allow for adaptation to workflow and automation on liquid-handling workstations.

Performance Data

Yield Comparison Mouse Tails

Yield Comparison Mouse Tails

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Comparison of Extract Yield: Mouse Tails

2 mm tail snips were extracted in 150 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 1.5 µL of each extract  was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.

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Yield Comparison Mouse Ears

Yield Comparison Mouse Ears

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Comparison of Extract Yield: Mouse Ears

1 mm diameter mouse ear punches were extracted in 100 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 5.0 µL of each extract  was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR  Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.

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Protocol Speed

Protocol Speed

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Protocol Speed

Extracta reduces time to results with a rapid, convenient protocol.

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Multiplex Genotyping

Multiplex Genotyping

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Multiplex Genotyping

Multiplex Genotyping

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Details

• Contents
  • Extraction Reagent (1 x 25 mL or 2 x 125 mL)
  • Stabilization Buffer (1 x 25 mL or 2 x 125 mL)
• Storage & Handling
  Store components at room temperature. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form
• Related Resources
  Product Manuals

  Extracta DNA Prep for Tissue

  Product Flyers

  Extracta DNA Prep

  CofA (PSF)

  PSF-95091-025-Lot#019399PSF-95091-250-Lot#016986PSF-95091-025-Lot#022062PSF-95091-025-Lot#023131PSF-95091-250-Lot#022333PSF-95091-250-Lot#023132PSF-95091-250-Lot#023133PSF-95091-025-Lot#023441PSF-95091-002-Lot#023548PSF-95091-002-Lot#023919PSF-95091-025-Lot#024458PSF-95091-025-Lot#025787PSF-95091-002-Lot#026417PSF-95091-250-Lot#026850PSF-95091-002-Lot#027063PSF-95091-025-Lot#026847PSF-95091-025-Lot#027064PSF-95091-250-Lot#027888PSF-95091-250-Lot#027920PSF-95091-025-Lot#027604PSF-95091-025-Lot#028154PSF-95091-250-Lot#028155PSF-95091-002-Lot#028731PSF-95091-002-Lot#030089PSF-95091-025-Lot#030042PSF-95091-002-Lot#030517PSF-95091-250-Lot#66139294PSF-95103-250Lot#030550PSF-95091-025-LOT#66140233PSF-95091-002-LOT#66141365

  Publications

  Parasitic nematodes of the genus Syphacia Seurat, 1916 infecting Muridae in the British Isles, and the peculiar case of Syphacia frederici

  Alex Stewart, Parasitology - 2017

  Abstract

  SUMMARY Syphacia stroma (von Linstow, 1884) Morgan, 1932 and Syphacia frederici Roman, 1945 are oxyurid nematodes that parasitize two murid rodents, Apodemus sylvaticus and Apodemus flavicollis, on the European mainland. Only S. stroma has been recorded previously in Apodemus spp. from the British Isles. Despite the paucity of earlier reports, we identified S. frederici in four disparate British sites, two in Nottinghamshire, one each in Berkshire and Anglesey, Wales. Identification was based on their site in the host (caecum and not small intestine), on key morphological criteria that differentiate this species from S. stroma (in particular the tail of female worms) and by sequencing two genetic loci (cytochrome C oxidase 1 gene and a section of ribosomal DNA). Sequences derived from both genetic loci of putative British S. frederici isolates formed a tight clade with sequences from continental worms known to be S. frederici, clearly distinguishing these isolates from S. stroma which formed a tight clade of its own, distinct from clades representative of Syphacia obvelata from Mus and S. muris from Rattus. The data in this paper therefore constitute the first record of S. frederici from British wood mice, and confirm the status of this species as distinct from both S. obvelata and S. stroma.

  The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea

  Emma Collins, Scientific Reports - 2019

  Abstract

  Insecticide resistance across sub-Saharan Africa may impact the continued effectiveness of malaria vector control. We investigated the association between carbamate and pyrethroid resistance with Anopheles gambiae s.l. parity, Plasmodium falciparum infection, and molecular insecticide resistance mechanisms in Guinea. Pyrethroid resistance was intense, with field populations surviving ten times the insecticidal concentration required to kill susceptible individuals. The L1014F kdr-N1575Y haplotype and I1527T mutation were significantly associated with mosquito survival following permethrin exposure (Prevalence Ratio; PR = 1.92, CI = 1.09–3.37 and PR = 2.80, CI = 1.03–7.64, respectively). Partial restoration of pyrethroid susceptibility following synergist pre-exposure suggests a role for mixed-function oxidases. Carbamate resistance was lower and significantly associated with the G119S Ace-1 mutation. Oocyst rates were 6.8% and 4.2% among resistant and susceptible mosquitoes, respectively; survivors of bendiocarb exposure were significantly more likely to be infected. Pyrethroid resistant mosquitoes had significantly lower parity rates than their susceptible counterparts (PR = 1.15, CI = 1.10–1.21). Our findings emphasize the need for additional studies directly assessing the influence of insecticide resistance on mosquito fitness.

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• Testimonials
  Extracta DNA Prep for PCR is one of the best, fastest and easiest to use reagent for genomic DNA isolation from human cells

  Scientist

  Deutsches Krebsforschungszentrum (DKFZ)

Ordering Information

Product
Kit Size
Order Info
• Extracta DNA Prep for PCR

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  25 mL

  Order (95091-025)

  250 mL

  Order (95091-250)

  2.5 mL

  Order (95091-002)