Extracta DNA Prep
Features & Benefits
- Simple, reagent-based system requires minimal technical skill
- Incubation step can be carried out in 96-well PCR plates or tubes using a standard DNA thermal cycler
- Compatible with a wide-range of clinical specimens, plant and animal tissues, and environmental samples
- Optional stabilization buffer allows for extended storage of extracted DNA templates
Extracta DNA Prep is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Yield Comparison Mouse Tails
Comparison of Extract Yield: Mouse Tails
2 mm tail snips were extracted in 150 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 1.5 µL of each extract was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.
Yield Comparison Mouse Ears
Comparison of Extract Yield: Mouse Ears
1 mm diameter mouse ear punches were extracted in 100 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 5.0 µL of each extract was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.
Extracta reduces time to results with a rapid, convenient protocol.
- Extraction Reagent (1 x 25 mL or 2 x 125 mL)
- Stabilization Buffer (1 x 25 mL or 2 x 125 mL)
Storage & HandlingStore components at room temperature. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form
Related ResourcesProduct ManualsProduct FlyersCofA (PSF)PSF-95091-025-Lot#019399PSF-95091-250-Lot#016986PSF-95091-025-Lot#022062PSF-95091-025-Lot#023131PSF-95091-250-Lot#022333PSF-95091-250-Lot#023132PSF-95091-250-Lot#023133PSF-95091-025-Lot#023441PSF-95091-002-Lot#023548PSF-95091-002-Lot#023919PSF-95091-025-Lot#024458PSF-95091-025-Lot#025787PSF-95091-002-Lot#026417PSF-95091-250-Lot#026850PSF-95091-002-Lot#027063PSF-95091-025-Lot#026847PSF-95091-025-Lot#027064PSF-95091-250-Lot#027888PSF-95091-250-Lot#027920PSF-95091-025-Lot#027604PSF-95091-025-Lot#028154PSF-95091-250-Lot#028155PublicationsParasitic nematodes of the genus Syphacia Seurat, 1916 infecting Muridae in the British Isles, and the peculiar case of Syphacia fredericiClick here to see all PublicationsAlex Stewart, Parasitology - 2017AbstractSUMMARY Syphacia stroma (von Linstow, 1884) Morgan, 1932 and Syphacia frederici Roman, 1945 are oxyurid nematodes that parasitize two murid rodents, Apodemus sylvaticus and Apodemus flavicollis, on the European mainland. Only S. stroma has been recorded previously in Apodemus spp. from the British Isles. Despite the paucity of earlier reports, we identified S. frederici in four disparate British sites, two in Nottinghamshire, one each in Berkshire and Anglesey, Wales. Identification was based on their site in the host (caecum and not small intestine), on key morphological criteria that differentiate this species from S. stroma (in particular the tail of female worms) and by sequencing two genetic loci (cytochrome C oxidase 1 gene and a section of ribosomal DNA). Sequences derived from both genetic loci of putative British S. frederici isolates formed a tight clade with sequences from continental worms known to be S. frederici, clearly distinguishing these isolates from S. stroma which formed a tight clade of its own, distinct from clades representative of Syphacia obvelata from Mus and S. muris from Rattus. The data in this paper therefore constitute the first record of S. frederici from British wood mice, and confirm the status of this species as distinct from both S. obvelata and S. stroma.