Extracta DNA Prep

Features & Benefits

• Simple, reagent-based system requires minimal technical skill
• Incubation step can be carried out in 96-well PCR plates or tubes using a standard DNA thermal cycler
• Compatible with a wide-range of clinical specimens, plant and animal tissues, and environmental samples
• Optional stabilization buffer allows for extended storage of extracted DNA templates

 

Extracta DNA Prep is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Extracta DNA Prep for PCR is a two-component reagent kit for rapid extraction of PCR-ready genomic DNA from a variety of tissues. Samples are processed in less than 30 minutes with minimal hands-on time and technical skill. Extracted genomic DNA is suitable for sensitive downstream PCR applications including end-point PCR, High Resolution Melt Analysis (HRM) and quantitative real-time PCR (qPCR) without requiring any additional clean-up. In addition, the extracted DNA may be used in multiplexed PCR applications such as transgene or knock-out analyses. Tissue extractions can be done in tubes, plates or deep-well blocks to allow for adaptation to workflow and automation on liquid-handling workstations.

Performance Data

Yield Comparison Mouse Tails

Yield Comparison Mouse Tails

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Comparison of Extract Yield: Mouse Tails

2 mm tail snips were extracted in 150 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 1.5 µL of each extract  was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.

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Yield Comparison Mouse Ears

Yield Comparison Mouse Ears

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Comparison of Extract Yield: Mouse Ears

1 mm diameter mouse ear punches were extracted in 100 µL of Extracta DNA Prep for PCR - Tissue or reagents from competitor products according to each manufacturers instructions. 5.0 µL of each extract  was used in 20 µL PCR reactions of mouse brain-derived neurotropic factor using PerfeCTa SYBR  Green FastMix. PCR data was obtained in triplicate and converted to number of copies of target detected using a standard curve generated from purified mouse genomic DNA.

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Protocol Speed

Protocol Speed

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Protocol Speed

Extracta reduces time to results with a rapid, convenient protocol.

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Multiplex Genotyping

Multiplex Genotyping

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Multiplex Genotyping

Multiplex Genotyping

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Details

• Contents
  • Extraction Reagent (1 x 25 mL or 2 x 125 mL)
  • Stabilization Buffer (1 x 25 mL or 2 x 125 mL)
• Storage & Handling
  Store components at room temperature. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form
• Related Resources
  Product Flyers

  Extracta DNA Prep

  Product Manuals

  Extracta DNA Prep for Tissue

  Publications

  Parasitic nematodes of the genus Syphacia Seurat, 1916 infecting Muridae in the British Isles, and the peculiar case of Syphacia frederici

  Alex Stewart, Parasitology - 2017

  Abstract

  SUMMARY Syphacia stroma (von Linstow, 1884) Morgan, 1932 and Syphacia frederici Roman, 1945 are oxyurid nematodes that parasitize two murid rodents, Apodemus sylvaticus and Apodemus flavicollis, on the European mainland. Only S. stroma has been recorded previously in Apodemus spp. from the British Isles. Despite the paucity of earlier reports, we identified S. frederici in four disparate British sites, two in Nottinghamshire, one each in Berkshire and Anglesey, Wales. Identification was based on their site in the host (caecum and not small intestine), on key morphological criteria that differentiate this species from S. stroma (in particular the tail of female worms) and by sequencing two genetic loci (cytochrome C oxidase 1 gene and a section of ribosomal DNA). Sequences derived from both genetic loci of putative British S. frederici isolates formed a tight clade with sequences from continental worms known to be S. frederici, clearly distinguishing these isolates from S. stroma which formed a tight clade of its own, distinct from clades representative of Syphacia obvelata from Mus and S. muris from Rattus. The data in this paper therefore constitute the first record of S. frederici from British wood mice, and confirm the status of this species as distinct from both S. obvelata and S. stroma.

  The relationship between insecticide resistance, mosquito age and malaria prevalence in Anopheles gambiae s.l. from Guinea

  Emma Collins, Scientific Reports - 2019

  Abstract

  Insecticide resistance across sub-Saharan Africa may impact the continued effectiveness of malaria vector control. We investigated the association between carbamate and pyrethroid resistance with Anopheles gambiae s.l. parity, Plasmodium falciparum infection, and molecular insecticide resistance mechanisms in Guinea. Pyrethroid resistance was intense, with field populations surviving ten times the insecticidal concentration required to kill susceptible individuals. The L1014F kdr-N1575Y haplotype and I1527T mutation were significantly associated with mosquito survival following permethrin exposure (Prevalence Ratio; PR = 1.92, CI = 1.09–3.37 and PR = 2.80, CI = 1.03–7.64, respectively). Partial restoration of pyrethroid susceptibility following synergist pre-exposure suggests a role for mixed-function oxidases. Carbamate resistance was lower and significantly associated with the G119S Ace-1 mutation. Oocyst rates were 6.8% and 4.2% among resistant and susceptible mosquitoes, respectively; survivors of bendiocarb exposure were significantly more likely to be infected. Pyrethroid resistant mosquitoes had significantly lower parity rates than their susceptible counterparts (PR = 1.15, CI = 1.10–1.21). Our findings emphasize the need for additional studies directly assessing the influence of insecticide resistance on mosquito fitness.

  The entomological impact of passive metofluthrin emanators against indoor Aedes aegypti: A randomized field trial

  Gregor J. Devine, PLOS Neglected Tropical Diseases - 2021

  Abstract

  Background In the absence of vaccines or drugs, insecticides are the mainstay of Aedes-borne disease control. Their utility is challenged by the slow deployment of resources, poor community compliance and inadequate household coverage. Novel application methods are required. Methodology and principal findings A 10% w/w metofluthrin “emanator” that passively disseminates insecticide from an impregnated net was evaluated in a randomized trial of 200 houses in Mexico. The devices were introduced at a rate of 1 per room and replaced at 3-week intervals. During each of 7 consecutive deployment cycles, indoor resting mosquitoes were sampled using aspirator collections. Assessments of mosquito landing behaviours were made in a subset of houses. Pre-treatment, there were no differences in Aedes aegypti indices between houses recruited to the control and treatment arms. Immediately after metofluthrin deployment, the entomological indices between the trial arms diverged. Averaged across the trial, there were significant reductions in Abundance Rate Ratios for total Ae. aegypti, female abundance and females that contained blood meals (2.5, 2.4 and 2.3-times fewer mosquitoes respectively; P<0.001). Average efficacy was 60.2% for total adults, 58.3% for females, and 57.2% for blood-fed females. The emanators also reduced mosquito landings by 90% from 12.5 to 1.2 per 10-minute sampling period (P<0.05). Homozygous forms of the pyrethroid resistant kdr alleles V410L, V1016L and F1534C were common in the target mosquito population; found in 39%, 24% and 95% of mosquitoes collected during the trial. Conclusions/Significance This is the first randomized control trial to evaluate the entomological impact of any volatile pyrethroid on urban Ae. aegypti. It demonstrates that volatile pyrethroids can have a sustained impact on Ae. aegypti population densities and human-vector contact indoors. These effects occur despite the presence of pyrethroid-resistant alleles in the target population. Formulations like these may have considerable utility for public health vector control responses.

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  CofA (PSF)

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• Testimonials
  Extracta DNA Prep for PCR is one of the best, fastest and easiest to use reagent for genomic DNA isolation from human cells

  Scientist

  Deutsches Krebsforschungszentrum (DKFZ)

Ordering Information

Product
Kit Size
Order Info
• Extracta DNA Prep for PCR

  Request Sample
  2.5 mL

  Order (95091-002)

  25 mL

  Order (95091-025)

  250 mL

  Order (95091-250)