Real-Time Quantitative PCR Reagents
PerfeCTa qPCR reagents combine a stringent, ultrapure antibody hotstart with performance engineered DNA polymerase in stabilized 1-tube formulations optimized for the specific performance needs of real-time quantitative PCR. Proprietary additives help eliminate persistent bubbles to enable efficient vortex mixing and fewer technical replicates thereby conserving precious sample. Adaptive buffer chemistry accommodates most assay designs and can be used with existing assay designs.
PerfeCTa qPCR ToughMix reagents contain proprietary inhibitor-neutralizing additives that relieve several types of PCR inhibition ensuring reliable qPCR assay performance with clinical, plant or environmental specimens or complex food matix.
Quantabio quantitative real-time PCR reagents are optimized to work in series with high-performance qScript RT reagents (for RNA targets) and Extracta DNA Prep (for DNA targets).
| SYBR Green Detection | Probe-based Detection | ||||||||
| PerfeCTa SYBR Green SuperMix | PerfeCTa SYBR Green FastMix | AccuStart Genotyping ToughMix | PerfeCTa qPCR FastMix UNG | PerfeCTa FastMix II | PerfeCTa qPCR ToughMix | PerfeCTa qPCR ToughMix, UNG | PerfeCTa Multiplex qPCR ToughMix | PerfeCTa Multiplex qPCR SuperMix | |
| Features | |||||||||
| Concentration | 2x | 2x | 2x | 2x | 2x | 2x | 2x | 5x | 2x |
| Sample Type | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA | gDNA, cDNA |
| Fast Cycling | No | Yes | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
| Multiplex Compatibility | N/A | N/A | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 2 targets | Up to 5 targets | Up to 4 targets |
| Carryover Contamination Control | N/A | N/A | N/A | Heat-labile UNG & blend of dTTP/dUTP | N/A | N/A | Heat-labile UNG & blend of dTTP/dUTP | N/A | N/A |
| Applications | |||||||||
| Gene Expression | x | x | x | x | x | x | x | x | |
| microRNA | x | x | |||||||
| ChIP | x | x | |||||||
| SNP Genotyping | x | ||||||||
| Inhibitor Tolerant | x | ** | x | x | |||||
| High Yield | x | x | x | ** | |||||
| Available Formats | |||||||||
| Master Mix | x | x | x | x | x | x | x | x | x |
| Packaging (Rxns/Units) | 250 Rxns 1250 Rxns 2000 Rxns |
250 Rxns 1250 Rxns 5000 Rxns |
250 Rxns 1250 Rxns 5000 Rxns |
250 Rxns 1250 Rxns 5000 Rxns |
250 Rxns 1250 Rxns 5000 Rxns |
250 Rxns 1250 Rxns 5000 Rxns |
250 Rxns 1250 Rxns 5000 Rxns |
250 Rxns 1000 Rxns 5000 Rxns |
50 Rxns 200 Rxns 1000 Rxns |
All Products
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PerfeCTa SYBR® Green FastMix
PerfeCTa SYBR® Green FastMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and DNA template. This rigorously optimized master mix contains of proprietary buffer technology, stabilizers and AccuFast Taq DNA polymerase to deliver maximum assay… -
PerfeCTa SYBR® Green SuperMix
PerfeCTa SYBR® Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum…