Reproducible Gene Expression Analysis
Meaningful gene expression analysis requires user-friendly solutions that reduce the potential for technical error. Quantabio’s gene expression solutions enable consistent and precise RNA detection assays with broad, linear dynamic detection ranges—even with limited amounts of starting material.
Workflow Solutions
Cells and tissues
![Detection](https://www.quantabio.com/wp-content/uploads/2021/10/Detection.png")
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Determine which gene expression solution is right for you.
RNA Solutions
| qScript Ultra SuperMix | qScript XLT 1-Step RT-qPCR ToughMix | UltraPlex 1-Step ToughMix | qScript 1-Step Virus ToughMix | |
|---|---|---|---|---|
| Features | ||||
| Kit Format | One tube | One tube | One tube | One tube |
| RT Enzyme | qScript Ultra RT | MMLV, reduced RNase activity | MMLV, reduced RNase activity | MMLV, reduced RNase activity |
| Concentration | 5x | 2x | 4x | 2x |
| Yield | **** | **** | **** | **** |
| Total RNA Input Range | 1pg – 2.5ug | 1pg – 100ng | 1pg – 100ng | 1pg – 100ng |
| Amplicon Length | 300 bp or less | 70 – 300 bp | 70 – 300bp | 70 – 300bp |
| Optimal Reaction Time | 10 min | N/A | N/A | N/A |
| Priming Method | ||||
| Oligo(dt) | * | |||
| Random | * | |||
| Gene Specific | * | * | * | |
| Applications | ||||
| Inhibitor Tolerant | * | * | * | |
| High Yield | * | * | * | * |
| Multiplex PCR | * | * | * | |
DNA Solutions
| PerfeCTa SYBR Green FastMix | PerfeCTa FastMix II | PerfeCTa qPCR SuperMix | |
|---|---|---|---|
| Features | |||
| Concentration | 2x | 2x | 2x |
| Sample Type | gDNA, cDNA | gDNA, cDNA | |
| Fast Cycling | Yes | Up to 2 targets | |
| Multiplex Compatibility | N/A | **** | N/A |
| Multiplex Compatibility | N/A | N/A | N/A |
| Applications | |||
| Gene Expression | * | * | * |
| microRNA | * | ||
| ChiP | * | ||
| SNP Genotyping | |||
| Inhibitor Tolerant | |||
| High Yield | |||
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