sparQ Universal Library Quant Kit
Fastest qPCR-based library quantification in 40 minutes
Features & Benefits
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sparQ Universal Library Quant Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Description
sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms. Accurate quantification of the number of amplifiable library molecules prior to loading onto a flow cell is a critical step in the NGS workflow and it ensures optimal cluster generation and cost-effective use of sequencing capacity.
The sparQ Universal Library Quant Kit uses real-time quantitative PCR (qPCR) to specifically quantify the number of library molecules that possess the appropriate adapter tag at each end.
Accurate library quantification in 40 minutes
sparQ Universal Library Quant Workflow

Complete library quantification solution with unmatched convenience
Reactions are prepared by simply adding standard or diluted library sample. Optimized protocols with fast cycling condition are provided for both 10 μl or 20 μl reaction volumes. Following qPCR run, a standard curve is constructed which is then used to determine the concentrations of each unknown diluted library sample.<
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- Supplied with all necessary, ready-to-use reagents to minimize
pipetting steps - Fast cycling protocols work for both 10 μl or 20 μl reaction volumes
- Reaction setup and data analysis templates and guides provided
qPCR as the most accurate method for library quantification
Performance comparison

Performance comparison
Results from sparQ and Roche KAPA Library Quant Kits were highly correlated. Concentrations of six different diluted libraries were determined using either the sparQ Universal Library Quant Kit on Quantabio Q qPCR cycler or the Roche KAPA Library Quantification Kit on Bio-Rad CFX following the manufacturer’s recommended protocol. Run times, including melt curves, were 40 minutes for sparQ and 80 minutes for KAPA.<
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- Highly correlated library quantification results obtained
- 50% faster run time compared to Roche KAPA Library Quant Kit
Lot-to-lot consistency of sparQ DNA Standards
High consistency of sparQ DNA Standards

High consistency of sparQ DNA Standards
sparQ Universal Library Quant Kits are manufactured with high lot-to-lot consistency. Concentrations of diluted libraries with low GC (library 1), high GC (library 2), or balanced GC-content (library 3) were determined using 3 different lots of sparQ DNA Standards. Each library sample was tested in quadruplicate reactions with each lot of sparQ DNA Standards. Standard deviations of average quantification values were all <0.13 pM.<
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- Rigorous quality control to ensure consistent, reliable performance
- Pre-diluted sparQ DNA Standards with excellent lot-to-lot consistency
Instrument Capability
sparQ Universal Library Quant Kit is optinized for use on any qPCR Instrument. A tube of ROX is included in the kit for qPCR instruments requiring higher concentrations of the reference dye. This unique formulation minimizes pipetting steps and ensures precise qPCR results.
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™
- Agilent AriaMx
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
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sparQ Universal Library Quant Kit
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