Features & Benefits

	Ultra-Fast Data Acquisition
	35 cycles in 25 minutes
	Unrivaled Performance
	Detect 2-fold expression level differences
	Portable & Compact
	4.5 lbs - transport without ever calibrating
	Scalable & Wireless
	Connect up to 10 instruments (48 samples/instrument)
	Magnetic Induction Technology
	Eliminate variability vs block-based cyclers

Q is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Q uses a patented magnetic induction technology to rapidly heat samples coupled with fan forced air for cooling to acquire data in only 25 minutes. Available in 2 or 4 channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

Q’s miniature speaker-size and 4.5 pound weight make it the most portable and versatile qPCR cycler on the market without ever needing to calibrate. Q also provides scalability as each instrument can process up to 48 samples per run and up to 10 Q’s can be connected to a single computer wirelessly via bluetooth enabling up to 480 samples to be processed simultaneously.

A key difference is that Q incorporates a unique spinning aluminum rotor providing superior temperature uniformity of
± 0.05°C versus traditional block-based real time cyclers which rely on multiple peltier heating blocks that can create edge effects resulting in sample variation. Not only does the data give you superior reproducibility, repeatability but enables detection of 2-fold gene expression level differences as well as identification of difficult class IV SNP’s requiring melt temperature resolutions of 0.1°C.

Who wouldn’t want to take one for a spin?

Ultra-Fast Data Acquisition

Generate high quality data, fast!

Ultra-Fast Data Acquisition

Ultra-Fast Data Acquisition

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Ultra-Fast Data Acquisition

5 point, 2x dilution series of Hepatitis B virus (HBV) cDNA template

Starting amount of 3E+06 copies (n = 4 each)

Efficiency = 90% (standard curve method); R² = 0.99

Time to complete run (including melt) = 26 min

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• Q’s speed is the fastest in the industry
• Don’t sacrifice on the performance quality of your qPCR
• Completing runs in as little as 25 minutes* is the new standard

*25 minute cycle times obtained with fast cycling master mixes and short amplicon assay designs targeting cDNA

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Unrivaled Performance

Detect 2-fold Differences

Unrivaled Performance

Unrivaled Performance

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Confidently detect small differences

1.2

Manganese superoxide dismutase gene (MnSOD)

Eight point, 2x dilution series of human genomic DNA (n = 4 each)

Efficiency = 98% (standard curve method)

R² = 1.00

1.3

Five point dilution series of HBV plasmid cDNA template (n = 4 each)

5 picogram difference between standards

Efficiency = 98% (standard curve method)

R² = 0.99

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• Confidently detect small differences
• High thermal uniformity and reproducibility
• Detect differences within a single cycle

Wide Linear Dynamic Range

Wide Linear Dynamic Range

Wide Linear Dynamic Range

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Wide Linear Dynamic Range

10 point, 10x dilution series of Hepatitis B virus (HBV) cDNA template Starting amount of 3E+09 copies (n = 3 each) over 10 logs Efficiency = 95% (standard curve method) R² = 0.99

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• Ultra-sensitive detection down to single digit copies of DNA
• Q performs reliably across a wide dynamic range

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Portable & Compact

A qPCR cycler the size of a speaker!

Q is the most portable and compact cyler on the market occupying ¼ the size of current qPCR platforms. The small footprint is enabled by the unique rotary and magnetic induction technology.

Portable & Compact

Portable & Compact

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A qPCR cycler the size of a speaker!

Don’t be fooled by its small size. Big things are happening inside.

• Fixed optics & no moving parts
• Never needs optical alignment or calibration
• No reference dyes or cross talk compensation required
• Utilizes proprietary 0.1 ml 4-strip tubes & caps
• supporting volumes of 5 – 30 μl
• High speed centrifugation ensures sample spin down
• Prevents evaporation & condensation with pre-loaded oil in tubes

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• Fixed optics & no moving parts
• Never needs optical alignment or calibration
• No reference dyes or cross talk compensation required
• Utilizes proprietary 0.1 ml 4-strip tubes & caps supporting volumes of 5 – 30 μl
• High speed centrifugation ensures sample spin down
• Prevents evaporation & condensation with pre-loaded oil in tubes

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Scalable & Wireless

Flexibility from 48 to 480 samples

Each Q cycler can process up to 48 samples per run and up to 10 cyclers can be connected to a single computer wirelessly to provide the desired scalability.

Scalable & Wireless

Scalable & Wireless

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Flexibility from 48 to 480 samples

Each Q cycler can process up to 48 samples per run and up to 10 cyclers can be connected to a single computer wirelessly to provide the desired scalability.
Q’s advanced data analysis software makes combining a single data set from multiple runs from multiple cylers seamlessly simple.
High reproducibility from thermal and optical performance ensures data from different runs and cyclers look like it was generated on the same instrument and the same run.
Lastly, anyone can setup and run the Q as it is plug-and play right of the box.

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• Combine single data sets from multiple runs from multiple cyclers
• Data from different runs and cyclers look a like
• Q is plug-and-play

Warranty Policy

Click Here for the Warranty Policy for Q qPCR Instruments

Performance Data

Ultra-Fast Data Acquisition

Ultra-Fast Data Acquisition

---

Generate high quality data, fast!

5 point, 2x dilution series of Hepatitis B virus (HBV) cDNA template

Starting amount of 3E+06 copies (n = 4 each)

Efficiency = 90% (standard curve method); R² = 0.99

Time to complete run (including melt) = 26 min

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>

Unrivaled Performanc

Unrivaled Performanc

---

Detect 2-fold Differences

Manganese superoxide dismutase gene (MnSOD)

Eight point, 2x dilution series of human genomic DNA (n = 4 each)

Efficiency = 98% (standard curve method)

R² = 1.00

---

<

>

Wide Linear Dynamic Range

Wide Linear Dynamic Range

---

Q provides ultra-sensitive detection down to single digit copies of DNA.

10 point, 10x dilution series of Hepatitis B virus (HBV) cDNA template

Starting amount of 3E+09 copies (n = 3 each) over 10 logs

Efficiency = 95% (standard curve method)

R² = 0.99

---

<

>

Portable & Compact

Portable & Compact

---

A qPCR cycler the size of a speaker!

Don’t be fooled by it’s small size. Big things are happening inside.

• Fixed optics & no moving parts
• Never needs optical alignment or calibration
• No reference dyes or cross talk compensation required
• Utilizes proprietary 0.1 ml 4-strip tubes & caps
• supporting volumes of 5 – 30 μl
• High speed centrifugation ensures sample spin down
• Prevents evaporation & condensation with pre-loaded oil in tubes

---

<

>

Scalable & Wireless

Scalable & Wireless

---

Flexibility from 48 to 480 samples

Each Q cycler can process up to 48 samples per run and up to 10 cyclers can be connected to a single computer wirelessly to provide the desired scalability.

Q’s advanced data analysis software makes combining a single data set from multiple runs from multiple cylers seamlessly simple.

High reproducibility from thermal and optical performance ensures data from different runs and cyclers look like it was generated on the same instrument and the same run.

Lastly, anyone can setup and run the Q as it is plug-andplay right of the box.

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Details

• Contents

  Specifications

  Front	Back

  Physical

  Height	5.1 in
  Width	5.9 in
  Length	5.9 in
  Weight	4.5 lbs

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  Thermal Performance

  Temperature Accuracy	±0.25°C
  Temperature Uniformity	±0.05°C
  Ramp Rates	Heating 4°C/s Cooling 3°C/s
  Temperature Input Range	40 – 99°C

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  Optical

  Detectors	Photodiode per channel
  Excitation Sources	High power LED for each channel
  Channels	Green	Ex 465nm	Em 510nm
  	Yellow	Ex 540nm	Em 570nm
  	Orange	Ex 585nm	Em 618nm
  	Red	Ex 635nm	Em 675nm
  Acquisition Time	1 second

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  Reaction Tubes

  Samples per Instrument	48
  Reaction Volume Range	5 – 30µL

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  Operating Environment

  Temperature	18 – 35ºC
  Relative Humidity	20 – 80%

• Related Resources
  Product Flyers

  Q FlyerDeveloping a Fast and Sensitive qPCR Assay to Improve Field-Based TB Testing in Africa

  Product Manuals

  Quick Start Guide

  Technical Notes

  Q 96 Well Plate CompatibilityApplication Note - Q qPCR Cycler & PerfeCTa SYBR Green FastMixQ in the Field - Alternative Power SourcesRemove Tubes from Last Run Application Note - Q qPCR Cycler & PerfeCTa NGS QuantificationRapid and accurate quantification of Illumina NGS libraries using the Q real-time qPCR Instrument Plugging in QComputer Recommendations for QQ qPCR Cycler Dye DetectionCompanion Fast QuantQuick Guide to AnalysisRapid and accurate quantification of Illumina NGS libraries using sparQ Fast Library Quant Kit on the Q real-time qPCR instrument

  FAQs

  How can I find help about the Q-qPCR?

  The best initial resource is the Q-qPCR manual. You can access the manual by clicking on the ‘?’ icon located at the top left hand side of the software, and selecting the Q-qPCR manual option. Once open, you can scroll to the relevant section or use the ctrl+F search function to find what you need.

  Which fluorophores can I use with the Q?

  The Q-qPCR’s optical detectors will work optimally with FAM (green), Cal Fluor Orange 560 (yellow detector), Cal Fluor Red 610 (orange channel) and Quasar 670 (red channel). This combination of fluorophores will produce less than 3% crosstalk between any/all channel/s used. Other fluorophores with similar spectral properties will also be suitable. For example, Texas Red/ROX have very similar spectral properties to Cal Fluor Red 610, and can be measure on the orange channel. A list of popular fluorophores that are suitable for a particular detector are available while setting up the assay chemistry, and a more comprehensive chart is provided in the Q-qPCR manual (page 143).

  Can I use the Q-qPCR for fieldwork?

  The Q-qPCR is well suited for fieldwork. You can run the machine from any power source, using a power inverter as required. Because you don’t need to calibrate for optics or temperature, and because of its small size/weight, the Q is ideal to transport to field sites while retaining functionality – it stays plug-and-play when other machines would need calibration. Also see our Technical Note: Q in the Field - Alternative Power Sources

  Can the samples be used for post-PCR analysis when the tubes have the oil overlay?

  Yes, you can break through the oil layer with a pipette tip to remove your PCR reaction without the oil.

  How fast can the Q complete a run?

  While this will vary according to your particular assay (different annealing temperatures and hold times), the Q can typically perform a 35 cycle qPCR run complete with melt curve in under 25 min.

  Click here to see all FAQs

  Publications

  Activation of LXR Receptors and Inhibition of TRAP1 Causes Synthetic Lethality in Solid Tumors

  Trang Thi Thu Nguyen, Cancers - 2019

  Abstract

  Cholesterol is a pivotal factor for cancer cells to entertain their relentless growth. In this case, we provide a novel strategy to inhibit tumor growth by simultaneous activation of liver-X-receptors and interference with Tumor Necrosis Factor Receptor-associated Protein 1 (TRAP1). Informed by a transcriptomic and subsequent gene set enrichment analysis, we demonstrate that inhibition of TRAP1 results in suppression of the cholesterol synthesis pathway in stem-like and established glioblastoma (GBM) cells by destabilizing the transcription factor SREBP2. Notably, TRAP1 inhibition induced cell death, which was rescued by cholesterol and mevalonate. Activation of liver X receptor (LXR) by a clinically validated LXR agonist, LXR623, along with the TRAP1 inhibitor, gamitrinib (GTPP), results in synergistic reduction of tumor growth and cell death induction in a broad range of solid tumors, which is rescued by exogenous cholesterol. The LXR agonist and TRAP1 inhibitor mediated cell death is regulated at the level of Bcl-2 family proteins with an elevation of pro-apoptotic Noxa. Silencing of Noxa and its effector BAK attenuates cell death mediated by the combination treatment of LXR agonists and TRAP1 inhibition. Combined inhibition of TRAP1 and LXR agonists elicits a synergistic activation of the integrated stress response with an increase in activating transcription factor 4 (ATF4) driven by protein kinase RNA-like endoplasmic reticulum kinase (PERK). Silencing of ATF4 attenuates the increase of Noxa by using the combination treatment. Lastly, we demonstrate in patient-derived xenografts that the combination treatment of LXR623 and gamitrinib reduces tumor growth more potent than each compound. Taken together, these results suggest that TRAP1 inhibition and simultaneous activation of LXR might be a potent novel treatment strategy for solid malignancies.

  Click here to see all Publications
• Testimonials
  We had the pleasure of testing out the ToughMix Mastermix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence.” As it’s name implies, the ToughMix stood up to its name and made the 38 hour trip from Houston to Mbabane, Swaziland and had beautiful Ct curves. Similarly, the Q PCR machine was an ease to travel with as carry on luggage. It’s 25 minute run gave Ct curves identical to our existing, and much bulkier, RT-PCR machine that was already on site in Swaziland. Considering it’s size, intuitive software, and near perfect reproducible Ct curves, we’ve decided to add the Q as our workhorse RT-PCR machine.

  Assistant Professor

  Infectious Diseases Global and
  Immigrant Health Baylor College of Medicine

  “Performing a LAMP assay using a Q-qPCR machine is really comparable with Genie II, however, the higher number of samples that can be analysed at a time and also the appearance of the first amplification signals 2 min earlier than those in Genie II, adds up to its value!”

  Steve Baeyen

  Flanders Research Institute for agriculture, fisheries and food

Ordering Information

Product
Kit Size
Order Info
• Q - qPCR Instrument
  Q 2-channel qPCR Instrument

  Order (95900-2C)

  Q 4-channel qPCR Instrument

  Order (95900-4C)

  1 year extended warranty 2-channel Q Instrument

  Order (95900-2CEXTWAR)

  1 year extended warranty 4-channel Q Instrument

  Order (95900-4CEXTWAR )
• Q Tubes & Caps (20 racks/box)
  20 racks of 48 tubes/box

  Order (95910-20)
• HRM License for Q qPCR Instrument
  1 HRM License

  Order (95915-20)

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