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Cloning

Quantabio Workflow Solutions for Cloning

Quantabio offers a range of high-quality products that are ideal for cloning applications. Our  DNA and RNA isolation reagents and amplification products are designed to provide reliable and consistent results, essential for successful cloning.

Extracta Plus DNA and RNA kits provide rapid extraction and purification of nucleic acids from a variety of sample sources with high and consistent yields. qScript Ultra Flex kit provides fast and accurate cDNA synthesis, while repliQa HiFi ToughMix offers ultra-fast, high-fidelity amplification, essential for successful cloning applications. Together, these products enable a fast and streamlined workflow, delivering high-quality cloning results with minimal optimization required.

  • Compatible with a variety of starting materials
  • cDNA synthesis of long transcript in 10 min
  • Ultra-Fast (1-10kb/s), high fidelity DNA amplification 

Cloning Workflow
Starting Material

Starting Material

E.g. Cells, Nucleic Acids…

 

DNA Extraction

DNA or RNA Extraction

Extracta Plus DNA

Extracta Plus RNA

Amplification

cDNA Synthesis & HiFi Amplification

qScript Ultra Flex Kit

repliQa HiFi ToughMix

PCR CleanUp

PCR CleanUp

sparQ PureMag Beads

 

Vector Assembly
Vector Assembly

E.g. Ligation, Gibson Assembly, etc…

 

Featured Products

Extracta Plus DNA
Product Details
Extracta Plus RNA
Product Details
qScript Ultra Flex Kit
Product Details
repliQa HiFi ToughMix
Product Details
sparQ PureMag Beads
Product Details
Customer Testimonials
repliQa HiFi ToughMix

"All I can say from testing repliQa HiFi ToughMix is that the results are jaw-dropping. The Platinum™ SuperFi II PCR Master Mix did nothing in the same amount of time under the same conditions. The assay I was testing out is a new way to amplify entire mitogenomes as a single amplicon (~15kb). This is a game changer, cutting the PCR time from ~8.5hrs to 1.5hrs, and way more product is generated. I am not often floored by data, but these results are amazing. I have another project I am working on with this assay and I am going to switch to this polymerase for sure. Good work Quantabio!"

A. K. | Post Doc, BCM
repliQa HiFi ToughMix

"I used NEB Q5 for the same plasmid for PCR, same annealing temperature. Only the repliQa HiFi toughMix gave me the correct size products. It is very fast and works much better than NEB Q5 for difficult plasmids in PCR."

Dr. Kara C. | UT Southwestern Medical Center
repliQa HiFi ToughMix

"This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."

Lili C. | Post Doc, USC
repliQa HiFi ToughMix

"The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."

Simon S. | Professor, Tennessee State University
repliQa HiFi ToughMix

"Worked great amplifying two gene target from plasmid DNA. It was the first time I have performed a two step reaction and it worked great. It was awesome running everything in 23 minutes too."

Dr. Brandon L. | University of Nebraska

 

Product Features

 

DNA and RNA Extraction

 

Extracta Plus DNA Extracta Plus RNA
Features    
Sample Type Fresh or frozen tissue, cells, blood, bacteria Fresh or frozen tissue, cells, blood
Analysis PCR, qPCR, Sanger Sequencing, NGS, Cloning RT-PCR, RT-qPCR, Sanger Sequencing, NGS, Cloning
Ideal for Sensitive downstream applications Sensitive downstream applications
Product Size 10 rxn 50 rxn 10 rxn, 50 rxn

cDNA synthesis

 

qScript Ultra Flex Kit
Features  
Kit Format Five Tubes (water included)
Concentration  5x
Total RNA input Range 1 pg – 2.5 µg
Amplicon Length up to 20 kb
Reaction Time 10 min
Priming Method Oligo(dT), Random or Gene Specific Priming
Product Size 25 rxn, 100 rxn, 500 rxn

HiFi Amplification

 

repliQa HiFi ToughMix
Features  
Concentration 2x
Amplicon Size Up to 24 Kb
Extension Time 1 – 10 sec / kb
Fidelity vs Taq 90x Taq
3′–>5′ exo Yes
Resulting Ends Blunt
Product Size 25 rxn, 100 rxn, 500 rxn

PCR Cleanup

 

sparQ PureMag Beads
Features  
Starting Material DNA or RNA, PCR products
Ideal for  Cleanup and size selection for both DNA and RNA
Downstream Application Cloning, NGS, RT-qPCR, Sanger Sequencing, etc.
Recovery Rate Up to 90% recovery for DNA 100bp
Product Size 5 ml, 60 ml, 450 ml
Resources

Want to learn more about Quantabio Workflow Solutions for Cloning? Here are some application notes and recent publications for further reading.

 

Fast and Efficient cDNA Synthesis and PCR Amplification for Molecular Cloning
Generating high quality dsDNA from RNA or DNA templates is the first and one of the most critical steps of cloning. In this application note, we detail the use of qScript Ultra Flex Kit and repliQa HiFi ToughMix to generate insert DNA for cloning in a fast and efficient way.
Read more
Simple and reliable in situ CRISPR-Cas9 nuclease visualization tool is ensuring efficient editing in Streptomyces species
Pšeničnik, A., Reberšek, R., Slemc, L., Godec, T., Kranjc, L., & Petković, H. (2022). Journal of microbiological methods, 200, 106545.
Read more
Generation of GLA-knockout human embryonic stem cell lines to model peripheral neuropathy in Fabry disease
Kaneski, C. R., Hanover, J. A., & Hoffman, U. H. S. (2022). Molecular Genetics and Metabolism Reports, 33, 100914.
Read more
Phasing of de novo mutations using a scaled-up multiple amplicon long-read sequencing approach
Holt, G. S., Batty, L. E., Alobaidi, B. K., Smith, H. E., Oud, M. S., Ramos, L.,& Veltman, J. A. (2022). Human Mutation, 43(11), 1545-1556.
Read more
Reconstruction of Full-length scFv Libraries with the Extended Range Targeted Sequencing Method
Wei, C., Criner, S., Sridhar, B., Shultzaberger, R., Looney, T., Fabani, M. M., & Glezer, E. N. (2022). bioRxiv, 2022-05.
Read more

Quantabio Sample Request

Select Your Assay

Starting Template

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Assay Format

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Detection Chemistry

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Multiplexing (more than 3 targets)

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Is gene-specific priming (GSP) required?

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What current Reverse Transcriptase or cDNA kit are you using?

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Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Analytik Jena qTower
  • Analytik Jena qTOWERiris
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
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Choose your application from the categories below

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Products

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