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COVID-19 Testing
COVID-19 Testing

COVID-19 Testing Solutions

To help address and fight the global pandemic caused by SARS-COV-2 since late 2019, Quantabio provides three essential RT-qPCR ToughTM master mixes to help research labs and testing providers eliminate extraction and purification while overcoming common PCR inhibitors to accurately detect COVID-19 at low limits of detection (LOD). 

Our RT-qPCR master mixes being used for COVID-19 in these latest developments

COVID 19 map

Reagents Manufactured for
+ 100 Million Tests

Quantabio proudly supports and partners with thousands of Testing labs in more than 37 countries around the world.

Workflow Solutions

Dried blood spots

Clinical/ Environmental sample

DNA Extraction

RNA Extraction
Extracta Plus RNA

Amplification

Amplification
RT-qPCR
Detection
Detection
qPCR Instrument

Featured Products

qScript XLT 1-Step RT-qPCR ToughMix
Product Details
UltraPlex 1-Step ToughMix
Product Details
qScript 1-Step Virus ToughMix
Product Details

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Determine which product is right for you.

 

qScript XLT 1-Step RT-qPCR ToughMix qScript 1-Step Virus ToughMix UltraPlex 1-Step ToughMix
Recommended in CDC protocol x x
Detection purified RNA x x x
Extraction-free detection x x
Saliva-direct detection x
Formulation 2X master mix 2X master mix 4X master mix
Taq Hot Start Yes Yes Yes
RT Hot Start No Yes Yes
Chemistry 1 tube SuperMix 1 tube SuperMix 1 tube SuperMix
Performance ++++ ++++ ++++
Cycling Conditions Fast or Standard qPCR Cycling Fast or Standard qPCR Cycling Fast or Standard qPCR Cycling
Multiplex Capabilty Up to 4 targets Up to 4 targets Up to 5 targets
Sensitivity +++ ++++ ++++
Inhibitor Tolerance Yes Yes Yes
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Customer Testimonials
UltraPlex 1-Step ToughMix

"Compared to other 1-step qRT-PCR mixes we have used, UltraPlex 1-Step ToughMix (4X) Low-ROX, 500R performs with the same sensitivity when testing using SARS-CoV-2-spiked saliva samples, but is easier to use and at a significantly lower price per reaction. The mix requires little to no time to thaw, decreasing the time required when preparing samples. The mix is also conveniently aliquoted into multiple tubes, reducing the chance for contamination as opposed to a single, high volume aliquot. Overall, these factors make this a better overall product as compared to competing companies (e.g., Thermo Fisher Scientific or Zymo) and we will continue to use this moving forward."

Rhoel R. D., PhD, MPH Professor of Infectious Diseases UF Eminent Scholar Director | CDC Southeastern Regional Center of Excellence for Vector Borne Diseases
qScript 1-Step Virus ToughMix

"1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment."

Guillermo C. | Project Coordinator Sick Kids Molecular Diagnostics Lab
News and Publications
Saliva DirectTM EUA Summary
UltraPlex 1-Step ToughMix Added to SalivaDirect - Emergency Use Authorization Protocol
SalivaDirectTM EUA Summary
Quantabio RT-qPCR reagents added to CDC Protocol
We are working tirelessly to increase our production capacities as it relates to the following RNA RT-qPCR reagents which are cited in the CDC protocol that has received FDA Emergency Use Authorization (EUA) approval.
Read article
CDC Protocol
CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel
CDC Protocol
Quantabio’s UltraPlex 1-Step ToughMix Continues to Enhance CDC Protocols to Detect and Differentiate COVID-19 and Influenza Respiratory Diseases
CDC’s latest emergency use authorization for influenza SARS-CoV-2 assay validates Quantabio’s ability to multiplex up to five targets with high efficiency, sensitivity and specificity.
Read More
UltraPlex® 1-Step ToughMix® for Extraction-free, High Capacity COVID-19 Testing
Nucleic acid amplification testing (NAAT) is becoming a preferred methodology for disease monitoring and more so during the current COVID-19 pandemic caused by SARS-CoV-2. This pandemic has infected >150 million people worldwide with more infectious strains evolving and being transmitted daily.
Read More
Extraction-free COVID-19 detection with Quantabio’s RT-qPCR ToughMixes
RT-qPCR is considered the “gold-standard” for COVID-19 nucleic acid detection. Detection from purified RNA provides the most sensitivity and reliability, but in the wake of increased demand for testing with faster turn-around-times, extraction-free detection ...
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Quantabio RT-qPCR ToughMix Given Expanded Role in CDC COVID-19 Testing Protocol
Amended CDC emergency use authorization recognizes UltraPlex formulation in an effective heat treatment alternative for RNA extraction.
Read More
Quantabio UltraPlex 1-Step ToughMix used for direct-lysis saliva SARS-CoV-2 detection
We evaluated UltraPlex 1-Step ToughMix (4X) in a direct-lysis (no extraction step) qPCR-based SARS-CoV-2 detection protocol from saliva samples.
Download Protocol
Customer Profile Story:
Detecting COVID-19 in wastewater using real-time PCR to predict and contain outbreaks (qScript XLT 1-Step RT-qPCR ToughMix)
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Publication:
Tracking SARS-CoV-2 in Sewage: Evidence of Changes in Virus Variant Predominance during COVID-19 Pandemic (qScript XLT 1-Step RT-qPCR ToughMix)
Read More

We’re Here to Help

Do you have questions about our products or their applications? Our experts are standing by for support.

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Select Your Assay

Starting Template

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Multiplexing (more than 3 targets)

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Is gene-specific priming (GSP) required?

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What current Reverse Transcriptase or cDNA kit are you using?

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Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Analytik Jena qTower
  • Analytik Jena qTOWERiris
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
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