qScript 1-Step Virus ToughMix
Features & Benefits
- Superior Sensitivity – higher yields optimized for lower viral RNA inputs
- Tough Tested - tolerant to a wide range of PCR inhibitors
- Multiplexing – Supports highly sensitive detection for up to four targets
- Broad Dynamic Range – Reliable data from your precious samples every time
qScript 1-Step Virus ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labeled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats. qScript 1-Step Virus ToughMix contains all required components for RT-qPCR except RNA template and primer/probe assays.
qScript 1-Step Virus ToughMix is powered by an engineered reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures, that includes a RT Hot-Start technology to suppresses non-specific primer extension by the RT before cDNA synthesis. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. These features combined with a stringent mAb hot-start Taq polymerase and ToughMix reagent technology to neutralize many common PCR inhibitors, provide reproducible low-copy quantification as well as extended room temperature stability of fully assembled reactions for automated reaction assembly.
qScript 1-Step Virus ToughMix does not contain an internal reference dye.
E Gene Comparison: Improvement of Low Copy RT-qPCR PerformanceqScript 1-Step Virus ToughMix was compared to the qScript XLT 1-Step RT-qPCR ToughMix.with the E Gene. Both mixes produced very good results with the former having slightly improved low copy RT-qPCR performance at both 150 and 15 copies of RNA.
Improvement of Low Copy RT-qPCR Performance
E Gene Comparison between ToughMixeE Gene comparing qScript 1-Step Virus ToughMix vs. qScript XLT 1-Step RT-qPCR ToughMix. 15, 150 and 1500 copies of RNA were compared. qScript 1-Step Virus ToughMix improves low copy RT-qPCR performance at both 150 and 15 copies of RNA.
- Improved low copy detection at both 150 and 15 copies of RNA
RdRp Gene ComparisonqScript 1-Step Virus ToughMix was compared to the qScript XLT 1-Step RT-qPCR ToughMix.with the RdRp Gene. Both mixes produced very good results with the former having slightly improved low copy RT-qPCR performance.
RdRp Gene Comparison
RDRP Gene ComparisonRdRp Gene comparing qScript 1-Step Virus ToughMix vs. qScript XLT 1-Step RT-qPCR ToughMix. 15, 150 and 1500 copies of RNA were compared. qScript 1-Step Virus ToughMix improves low copy RT-qPCR performance.
- Similar but slightly improved low copy RT-qPCR performance
- 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride,
- qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,
- AccuVue blue qPCR dye, and stabilizers
Storage & HandlingStore components in a constant temperature freezer at -25°C to -15°C.
Repeated freezing and thawing do not affect RT-qPCR performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
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