qScript 1-Step Virus ToughMix

Superior sensitivity for viral RNA detection

Features & Benefits

  • Superior Sensitivity – higher yields optimized for lower viral RNA inputs
  • Tough Tested - tolerant to a wide range of PCR inhibitors
  • Multiplexing – Supports highly sensitive detection for up to four targets
  • Broad Dynamic Range – Reliable data from your precious samples every time

 

qScript 1-Step Virus ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labeled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats. qScript 1-Step Virus ToughMix contains all required components for RT-qPCR except RNA template and primer/probe assays.

qScript 1-Step Virus ToughMix is powered by an engineered reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures, that includes a RT Hot-Start technology to suppresses non-specific primer extension by the RT before cDNA synthesis. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. These features combined with a stringent mAb hot-start Taq polymerase and ToughMix reagent technology to neutralize many common PCR inhibitors, provide reproducible low-copy quantification as well as extended room temperature stability of fully assembled reactions for automated reaction assembly.

qScript 1-Step Virus ToughMix does not contain an internal reference dye.

E Gene Comparison: Improvement of Low Copy RT-qPCR Performance

qScript 1-Step Virus ToughMix was compared to the qScript XLT 1-Step RT-qPCR ToughMix.with the E Gene. Both mixes produced very good results with the former having slightly improved low copy RT-qPCR performance at both 150 and 15 copies of RNA.

Improvement of Low Copy RT-qPCR Performance


E Gene Comparison between ToughMixe

E Gene comparing qScript 1-Step Virus ToughMix vs. qScript XLT 1-Step RT-qPCR ToughMix. 15, 150 and 1500 copies of RNA were compared. qScript 1-Step Virus ToughMix improves low copy RT-qPCR performance at both 150 and 15 copies of RNA.


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  • Improved low copy detection at both 150 and 15 copies of RNA


RdRp Gene Comparison

qScript 1-Step Virus ToughMix was compared to the qScript XLT 1-Step RT-qPCR ToughMix.with the RdRp Gene. Both mixes produced very good results with the former having slightly improved low copy RT-qPCR performance.

RdRp Gene Comparison


RDRP Gene Comparison

RdRp Gene comparing qScript 1-Step Virus ToughMix vs. qScript XLT 1-Step RT-qPCR ToughMix. 15, 150 and 1500 copies of RNA were compared. qScript 1-Step Virus ToughMix improves low copy RT-qPCR performance.


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  • Similar but slightly improved low copy RT-qPCR performance

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What Customers Say
qScript 1-Step Virus ToughMix performed BETTER than other 3 mixes and amplified very well the S target from SARS-CoV-2 in a highly multiplex environment

G. Casallo
Sick Kids - Molecular Diagnostics Lab

Details

  • Contents
    • 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride,
    • qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,
    • AccuVue blue qPCR dye, and stabilizers
  • Storage & Handling
    Store components in a constant temperature freezer at -25°C to -15°C.
    Repeated freezing and thawing do not affect RT-qPCR performance.
    For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
  • Instrument Capability
    ROX
    • Applied Biosystems 5700
    • Applied Biosystems 7000
    • Applied Biosystems 7300
    • Applied Biosystems 7700
    • Applied Biosystems 7900
    • Applied Biosystems 7900HT
    • Applied Biosystems 7900 HT Fast
    • Applied Biosystems StepOne™
    • Applied Biosystems StepOnePlus™
    Low ROX
    • Applied Biosystems 7500
    • Applied Biosystems 7500 Fast
    • Stratagene Mx3000P®
    • Stratagene Mx3005P™
    • Stratagene Mx4000™
    • Applied Biosystems ViiA 7
    • Applied Biosystems QuantStudio™
    • Agilent AriaMx
    • Douglas Scientific IntelliQube®
    No ROX
    • Quantabio Q
    • BioRad CFX
    • Roche LightCycler 480
    • QIAGEN Rotor-Gene Q
    • Other
    Bio-Rad iCycler iQ systems
    • BioRad iCycler iQ™
    • BioRad MyiQ™
    • BioRad iQ™5
  • Related Resources