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sparQ PureMag Beads

Fast, reliable DNA purification & size selection for NGS workflows

Features & Benefits

  • High recovery of DNA fragments greater than 100 bp
  • Efficient removal of unwanted components from adapter ligation and PCR reactions
  • Consistent single or double-sided size selection
  • Seamless integration into existing NGS workflows with little or no protocol change
  • Easy-to-use and compatible with manual processing or automated liquid handling robots
  • Cost effective alternative to AMPure® XP with equivalent performance

Description

sparQ PureMag Beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads, this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag Beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection

Double-sided size selection is used to remove smaller and larger fragments from either side of the desired region. The fragment size can be easily adjusted to suit the application by manipulating the sparQ PureMag Beads to DNA volumetric ratio.

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  • Double-sided size selection tunable for specific application
  • Achieve consistent size range by simply manipulating the beads to sample ratio

Performance Data

Consistent and tunable size selection

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Electropherogram of fragmented human genomic DNA pre- and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.

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Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

Highly reproducible DNA library profiles were achieved using different lots of sparQ PureMag Beads and a broad range of input amount. Libraries were prepared with sparQ DNA Library Prep Kit from 100 ng and 1 ng of fragmented microbial genomic DNA. sparQ PureMag Beads were used post adapter ligation and PCR amplification to effectively remove adapter-dimers and primer-dimers.

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Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP

sparQ PureMag Beads show equivalent performance to AMPure XP for DNA purification. 50 bp DNA ladder was purified with sparQ PureMag Beads and AMPure XP at different beads to DNA ratios and analyzed on 2% agarose gel.

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Performance Data

Consistent and tunable size selection

Consistent and tunable size selection

sparQ PureMag beads workflow

Double-sided size selection is used to remove smaller and larger fragments from either side of the desired region. The fragment size can be easily adjusted to suit the application by manipulating the sparQ PureMag Beads to DNA volumetric ratio.

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Consistent and tunable size selection

Consistent and tunable size selection

Bioanalyzer trace of fragmented human genomic DNA prior and post double-sided size selection

Electropherogram of fragmented human genomic DNA prior and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.

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Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs

Highly reproducible DNA library profiles were achieved using different lots of sparQ PureMag Beads and a broad range of input amount. Libraries were prepared with sparQ DNA Library Prep Kit from 100 ng and 1 ng of fragmented microbial genomic DNA. sparQ PureMag Beads were used post adapter ligation and PCR amplification to effectively remove adapter-dimers and primer-dimers.

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Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP

sparQ PureMag Beads show equivalent performance to AMPure XP for DNA purification. 50 bp DNA ladder was purified with sparQ PureMag Beads and AMPure XP at different beads to DNA ratios and analyzed on 2% agarose gel.

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Featured Publication

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What Customers Say
Beads performed as well in my tests as Ampure XP which I consider to be the gold standard. Impressive results given the lower price... Read More Beads performed as well in my tests as Ampure XP which I consider to be the gold standard. Impressive results given the lower price for sparQ PureMag Beads."
Director of Sequencing Technology
University of Southern California

Details

  • Contents
    Cat. No 95196-005 Size:    5 mL
      95196-060    60 mL
      95196-450 450 mL
  • Storage & Handling

    Store the product in a constant temperature refrigerator at 2°C to 8°C upon receipt. Please do not freeze. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

  • Related Resources
    Product Flyers
    sparQ PureMag Beads Flyer
    Product Manuals
    sparQ PureMag Beads
    Technical Notes
    Streamlined single-tube solutions for high quality DNA library preparation
    FAQs
    Can I still use the sparQ PureMag Beads if I left it at room temperature for some time?
    sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.
    Can I still use the sparQ PureMag Beads if it was accidentally frozen?
    sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.
    How do I dry my samples before elution?
    Air-dry the beads on the magnetic stand for 5 min or until the beads are dry and before they start to crack. Do not over-dry the beads as it can reduce the DNA recovery.
    Is there a size limit for the DNA fragments that can be bound with sparQ PureMag Beads?
    sparQ PureMag Beads will bind DNA fragments 100 bp and above and exclude primers 50 bases and below. There will be some recovery for oligo or DNA between 50 and 100 bases.
    What is the recovery rate with sparQ PureMag Beads?
    The recovery rate depends on many factors, including the fragment size, sample volume, sample concentration, and elution volume. Up to 90% recovery rate can be expected under the optimal condition. The yield is also correlated with the ratio of beads to sample, and the lower ratio usually results in lower yield. When the ratio reaches to 1.8X, it generally produces maximal recovery.
    Click here to see all FAQs
    Publications
    A rapid, low cost, and highly sensitive SARS-CoV-2 diagnostic based on whole genome sequencing
    Brian Glenn St Hilaire, BioRxiv - 2020
    Abstract
    Early detection of infection with SARS-CoV-2 is key to managing the current global pandemic, as evidence shows the virus is most contagious on or before symptom onset1,2. Here, we introduce a low-cost, high-throughput method for diagnosis of SARS-CoV-2 infection, dubbed Pathogen-Oriented Low-Cost Assembly & Re-Sequencing (POLAR), that enhances sensitivity by aiming to amplify the entire SARS-CoV-2 genome rather than targeting particular viral loci, as in typical RT-PCR assays. To achieve this goal, we combine a SARS-CoV-2 enrichment method developed by the ARTIC Network (https://artic.network/) with short-read DNA sequencing and de novo genome assembly. We are able to reliably (>95% accuracy) detect SARS-CoV-2 at concentrations of 84 genome equivalents per milliliter, better than the reported limits of detection of almost all diagnostic methods currently approved by the US Food and Drug Administration. At higher concentrations, we are able to reliably assemble the SARS-CoV-2 genome in the sample, often with no gaps and perfect accuracy. Such genome assemblies enable the spread of the disease to be analyzed much more effectively than would be possible with an ordinary yes/no diagnostic, and can help identify vaccine and drug targets. Using POLAR, a single person can process 192 samples over the course of an 8-hour experiment, at a cost of ~$30/patient, enabling a 24-hour turnaround with sequencing and data analysis time included. Further testing and refinement will likely enable greater enhancements in the sensitivity of the above approach.
    Influence of introduced arbuscular mycorrhizal fungiand phosphorus sources on plant traits, soil properties,and rhizosphere microbial communities in organiclegume-flax rotation
    Yunliang Li, Plant Soil - 2019
    Abstract
    Aims We identify P management strategies combiningarbuscular mycorrhizal fungal (AMF) inoculation withrock phosphate or composted manure for intensive or-ganic grain-production systems.Methods We measured the response of plants traits andsoil properties to the factorial combination of three ratesof organic-approved P sources applied in rotation phase-1of legume–flax cropping systems, and of granular AMFinoculant applied in the first, second, or both rotationphases, or not applied. Treatment combinations effectson the rhizosphere communities of AMF, fungi, andbacteria were tested by amplicon sequencing, in twopedoclimates.Results Inoculation had limited effects in both environ-ments. Composted manure decreased lentil yield, butincreased lentil N and P concentrations and soil P fertilityon the Chernozem, while increasing pea productivity onthe Luvisol. Composted manure applied in rotationphase-1 had a residual effect on flax productivity, N andP concentrations, and soil P fertility in both environ-ments. Rock phosphate reduced soil P fertility and flaxproductivity on the Gray Luvisol. The βdiversity of therhizosphere communities was unaffected by treatments,while the αdiversity of bacteria and AMF was altered byAMF inoculation and fertilization only in the GrayLuvisol. Correlations between microbial species andplant traits or soil properties were inconsistent, reflectingthe complex relationships among microbial community,plant identity, and environmental conditions.Conclusion Here, composted manure was more influ-ential than AMF inoculation and rock phosphate. Giventhe influence of environmental conditions, small fieldtrials are recommended before wide-scale adoption oftheir use.
    Click here to see all Publications
    CofA (PSF)
    PSF-95196-005-Lot#027837PSF-95196-060-Lot#027840PSF-95196-450-Lot#029004PSF-95196-450-Lot#029196PSF-95196-005-Lot#029400PSF-95196-450-Lot#029401PSF-95196-005-LOT#66152722PSF-95196-060-LOT#66152723PSF-95196-450-LOT#66152724
    Customer Profile Stories
    Biolegend Customer Profile Story
  • Testimonials

    “Very good product for NGS library purification and size selection”

    Senior Scientist

    MD Anderson

    "Great product. Useful for many different types of DNA capture applications."

    Associate Professor

    University of South Carolina

    "Worked exactly as advertised. I was able to get complete recovery of my DNA easily and quickly."

    Postdoctoral Fellow

    BC CANCER RESEARCH CENTRE <BCCRC>

    "Very good product for NGS Library purification and size selection"

    Associate Professor

    UNIVERSITY of TEXAS MD ANDERSON CANCER CENTER

    "Very easy to use and really good recovery yield."

    Biologist

    BATJ, Inc

    "Beads performed as well in my tests as Ampure XP which I consider to be the gold standard.  Impressive results given the lower price for sparQ PureMag Beads."

    Director of Sequencing Technology

    University of Southern California

    "We found that size selection with sparQ PureMag Beads was similar to our current vendor for but the amount of size selected library recovered was significantly larger."

    Vice President of Operations

    PHASE GENOMICS

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