“Very good product for NGS library purification and size selection”
sparQ PureMag Beads
Features & Benefits
- High recovery of DNA and RNA fragments
- Efficient removal of unwanted components from adapter ligation and PCR reactions
- Consistent single or double-sided size selection
- Seamless integration into existing NGS workflows with little or no protocol change
- Easy-to-use and compatible with manual processing or automated liquid handling robots
- Cost effective alternative to AMPure® XP, SPRI Select, and RNAClean XP with equivalent performance
Description
Workflow for Double-Sided Size Selection
Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection
Double-sided size selection is used to remove smaller and larger fragments from either side of the desired region. The fragment size can be easily adjusted to suit the application by manipulating the sparQ PureMag Beads to DNA volumetric ratio.
- Double-sided size selection tunable for specific application
- Achieve consistent size range by simply manipulating the beads to sample ratio
DNA Cleanup and Size Selection
- Efficient recovery of DNA equivalent to AMPure XP
- Highly reproducible purification across a range of inputs
- Consistent and tunable size selection
Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection
Electropherogram of fragmented human genomic DNA pre- and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.
Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs
Highly reproducible DNA library profiles were achieved using different lots of sparQ PureMag Beads and a broad range of input amount. Libraries were prepared with sparQ DNA Library Prep Kit from 100 ng and 1 ng of fragmented microbial genomic DNA. sparQ PureMag Beads were used post adapter ligation and PCR amplification to effectively remove adapter-dimers and primer-dimers.
Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP
sparQ PureMag Beads show equivalent performance to AMPure XP for DNA purification. 50 bp DNA ladder was purified with sparQ PureMag Beads and AMPure XP at different beads to DNA ratios and analyzed on 2% agarose gel.
RNA Cleanup and Size Selection
- Efficient recovery of RNA equivalent to RNAClean XP
- Tunable size selection
- Demonstrated RNase-free
Recovery of RNA fragments

Recovery of RNA fragments
RNA recovery efficiency. 500 ng or 50 ng ssRNA ladder was purified using 1.8X sparQ PureMag Beads or RNAClean XP. RNA was quantified by Nanodrop. Bars show mean average ± s.d. of two experiments.
Size selection of RNA

Size selection of RNA
Size selection of RNA with sparQ PureMag Beads. ssRNA ladder was purified with 1X (blue), 0.8X (orange) or 0.6X (red). The eluted RNA, and a control sample of RNA ladder (black), was diluted 10-fold and analyzed on a TapeStation RNA High Sensitivity Screen Tape. An overlay of electropherograms for each sample is shown with labelled peaks.
RNA Stability

RNA Stability
RNA stability with sparQ PureMag Beads compared to RNAClean XP. ssRNA ladder was mixed with 1X sparQ PureMag Beads or RNAClean XP and either cleanup carried out immediately (0 h) or the mixture incubated at room temperature for 4 h before cleanup (4 h). ssRNA was incubated for 4 h with saliva as a positive control (+ve) or RNase-free water as a negative control (-ve). Combined digital gel images for TapeStation High Sensitivity tape analyses are shown.
Room Temperature Stability
- Equivalent recovery and size selection when stored at stored at room temperature for 6 months vs. 4ºC.
Comparison of recovery from cleanup

Comparison of recovery from cleanup
Electropherogram of fragmented human genomic DNA pre- and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.
Size selection at different storage conditions

Size selection at different storage conditions
Size selection of DNA with sparQ PureMag Beads. Beads were stored at 4°C or room temperature (RT) for six months then used for size selection of 50 bp DNA ladder. Figure shows overlay of representative digital electropherogram images of 50 bp DNA ladder (green), or DNA eluted from size selection using beads stored at 4°C (blue) or room temperature (orange).
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sparQ PureMag Beads