qScript XLT 1-Step RT-qPCR ToughMix
- Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin
- Flexible – Use fast or standard qPCR cycling conditions
- Broad dynamic range- Reliable data from your precious samples every time
- Multiplexing enabled – Supports highly sensitive detection for up to four targets
qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
qScript XLT 1-Step RT-qPCR ToughMix ROX
qScript XLT 1-Step RT-qPCR ToughMix
qScript XLT 1-Step RT-qPCR ToughMix Low-ROX
Description
qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5'-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.
This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.
Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
We used the qScript XLT 1-step RT-qPCR ToughMix to reliably and very quickly identify an experimental drug candidate and demonstrate its efficacy to correct the debilitating effect of an inherited disease. The immediate success and impeccable performance of this mix shortened our labs time to result and confirmation easily by 2-3 months.
Details
2X concentrated One-Step Master Mix containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- qScript XLT reverse transcriptase
- RNase inhibitor protein
- AccuStart II Taq DNA Polymerase
- Inert AccuVue™ plate loading dye
- Proprietary enzyme stabilizers and performance-enhancing additives
- Titrated reference dye (if applicable)
Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not affect RT-qPCR performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Instrument Capability
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Customer Testimonials
"qScript XLT One-Step RT-qPCR ToughMix is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."
Details
2X concentrated One-Step Master Mix containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
- qScript XLT reverse transcriptase
- RNase inhibitor protein
- AccuStart II Taq DNA Polymerase
- Inert AccuVue™ plate loading dye
- Proprietary enzyme stabilizers and performance-enhancing additives
- Titrated reference dye (if applicable)
Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not affect RT-qPCR performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Instrument Capability
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Resources
Customer Profile Stories
Flyers
Product Manuals
Application Notes
CofA (PSFs)
SDSs
Publications
Customer Testimonials
"qScript XLT One-Step RT-qPCR ToughMix is an easy kit that contains all the reagents needed for the master mix in one tube. The only components I added were my primers, probe and nuclease free water. The protocol is short and user friendly."