sparQ UDI Adapters (1-96)
1 reviews
Dual-indexed barcoded adapters for DNA and RNA libraries for Illumina®
Features & Benefits
- Flexible pooling: multiplex up to 96 samples per sequencing run
- Improved performance: prevents index hopping and enhances demultiplexing accuracy
- Optimized: for maximum ligation efficiency with sparQ library prep kits
- Multiple applications: including whole genome (with amplification or PCR-free), target enrichment, whole transcriptome sequencing and many more
sparQ UDI Adapter is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Product
Kit Size
Part Number
Price
Quantity
sparQ UDI Adapters
Kit Size:
96 Unique Adapters
Part Number:
95211-096
$899.00
Kit Size:
Sample size only (1 - 24)
Part Number:
95211-024
$0.00
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Description
sparQ UDI Adapters (1-96) are unique dual-indexed adapters designed to be used on Illumina sequencing platforms. It is compatible with both DNA and RNA libraries. The use of UDI’s minimizes index hopping events while also enabling greater multiplexing on flow cells.
Details
Storage & Handling
Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or product Specification Form.
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The kit is as easy to use, and the library yields are as good as my current kit. However, one of my smaller libraries (~300 bp) shows artificial larger species (two bands and smears between 300 and 500 bp) on D1000 TapeStation after adapter ligation and PCR. There are also extra bands appearing between 100 and 200 bp that are absent in my current method, which may stem from the adapters used and PCR error.