Qscript lyo 1-step
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Lyophilized Single Tube Format |
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High Sensitivity & Specificity |
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Wide Dynamic Range |
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Superior Multiplexing |
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Eliminate Freezer Storage |
Qscript lyo 1-step is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Qscript lyo 1-step
Description
Qscript lyo 1-step is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes. The reagent contains a hot-start thermo-stable polymerase, a genetically engineered reverse transcriptase as well as other components to ensure higher performance detection of up to 5 targets with maximum sensitivity and specificity. The enhanced stability of the freeze-dried master mix enables convenient shipping and storage at room temperature. The single tube reaction facilitates easy reaction set up while preventing potential cross-contamination.
Details
Instrument Compatibility
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Analytik Jena qTower
- Analytik Jena qTOWERiris
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Performance Data
Multiplex up to 5 targets per assay
Qscript lyo 1-step was tested with a 5-plex assay with 7 serial dilutions of Human total RNA (4x from 50 ng to 30 pg). 20 μl of primers/probes was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.
High performance across a range of inputs
Qscript lyo 1-step was tested with 12 serial dilutions of Human total RNA (4x from 1250 ng to 0.3 pg). 20 μl of LDHA primer/probe was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.
High sensitivity & specificity
Qscript lyo 1-step demonstrates higher sensitivity and fluorescence detection than a market-leading one-step RT-qPCR product (liquid). The figure shows an LDHA assay with 10-fold serial diluted universal human RNA (50 ng to 0.5 pg) as template. 20 μl of LDHA primer/probe was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.
Convenient reaction setup
Just add RNA, primers and probes
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