eQo 1-Step ToughMix®
| 5 star | 40% | |
| 4 star | 60% | |
| 3 star | 0% | |
| 2 star | 0% | |
| 1 star | 0% |
Unparalleled performance meets sustainability.
- Unrivaled Sensitivity – Detection down to 2 copies of RNA
- Sustainable & Earth Friendly – Lyophilized eQo bead format eliminates dry ice, CO2 consumptions & reduces shipping costs
- Novel Reverse Transcriptase – Convenient, ambient reaction setup. Overcomes rigid RNA secondary structure
- High Stability – Up to 1 year at room temperature, up to 1 year rehydrated at -20°C
- Tough Tested – Broad and significantly enhanced tolerance to PCR inhibitors
eQo 1-Step ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
eQo 1-Step ToughMix
Description
eQo 1-step ToughMix® is a lyophilized reagent system for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes. It is supplied with a proprietary rehydration buffer that when combined with the lyophilized “eQo beads” produces a stabilized 4X concentrated master mix. The kit includes thermolabile UDG for amplicon carryover elimination, an enhanced warm start reverse transcriptase (RT) and all other required components for 1-step RT-qPCR except RNA template and primer/probe(s). The reaction chemistry has been optimized for inhibitor tolerance and delivers exceptional performance in either single-plex or highly demanding multiplex 1 step RT-qPCR formats.
Watch our Webinar: Merging high performance with sustainable design:
Introducing eQo 1-Step ToughMix, a lyophylized solution for denmanding RNA quantification applications
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Details
Component Description |
95301-100 |
95301-01K |
Master Mix Pouch (contains 1 tube of lyophilized beads plus a dessicant packet) |
100 reaction packet |
1000 reaction packet |
Rehydration Buffer |
1 x 0.55 ml |
1 x 5.5 ml |
Performance Data
Amplification of a virioid RNA with a high degree of stable secondary structure.
Dahlia latent virioid RNA template was prepared, and upon folding, the ssRNA molecule was shown to adopt a stable rod-like structure. Compared with kits from other suppliers, reactions with eQo 1-step carried out at a reverse transcription
Inhibitor resistance across wide range of sample types.
Innate inhibitor tolerance of qScript Ultra reverse transcriptase together with the formulation of the eQo 1-step mix allow for superior tolerance to many common reaction inhibitors. Reverse transcription was carried out at manufacturer’s recommendations (including 60°C for eQo 1-Step); thermocycling was performed at the same cycling protocols for all. Cq values were compared with reactions without inhibitors to show relative inhibitor tolerance in the 1-step reaction.
Gene expression quantification using primer limiting of GC-rich targets.
1-step reaction mixtures were assembled in duplicate using 50 ng total human RNA and the indicated primer/probe assay sets. Reverse transcription was carried out at manufacturer’s recommendations (including 60°C for eQo 1-Step); thermocycling was performed at the same cycling protocols for all The average Cq was plotted for each gene expression assay.
Improved limits of detection for RNA pathogens
A representative amplification plot was generated by diluting SARS-CoV-2 RNA from 200,000 to 2 copies. The inset plot displays the average Cq against the log copy number of the SARS-CoV-2 target. Strong amplification was observed even at the highest dilution.
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Customer Product Reviews
| 5 star | 40% | |
| 4 star | 60% | |
| 3 star | 0% | |
| 2 star | 0% | |
| 1 star | 0% |
eQo 1-Step ToughMix®
On our very limited first evaluation, we were satisfied with the overall performance comparable to our Current custom Tough Mix. We are going to do further evaluations on some of our problematic assays. Seemed like the Delta Rn results were superior!
We compared this to Qiagne multiplex reagents. This product is cheap and easy to use.
The robustness of the MM was good but the efficiency was slightly worst than what we are used to. Would consider it since the price point is very competitive.