AccuStart II GelTrack® PCR SuperMix
- Contains premixed loading dye to streamline gel electrophoresis
- User-friendly 2X concentrated master mix simplifies reaction setup with exceptional room-temperature stability (≥30 days at 22°C) and withstands to repetitive freeze-thaw (≥ 20X)
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
- Stringent, ultrapure antibody hotstart ensures sensitive and precise assay performance
AccuStart II GelTrack PCR SuperMix
Description
AccuStart II GelTrack® PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. This kit improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart II Taq DNA polymerase which contains ultra-pure monoclonal antibodies that arrest polymerase prior to the initial PCR denaturation step. Hot start antibodies are rapidly and irreversibly denatured (1 minute at 94ºC), releasing a high yielding Taq DNA polymerase mutant with absolute maximum processivity and velocity from the first cycle. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
GelTrack Loading Dye is a mixture of blue and yellow electrophoresis-tracking dyes that migrate at approximately 4kb and 50 bp, and comes pre-mixed with the PCR reagents.
Details
2X reaction buffer containing
- MgCl2 (3 mM)
- dATP, dCTP, dGTP, dTTP (0.4 mM each)
- AccuStart II Taq DNA Polymerase
- GelTrack loading dye and stabilizers.
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AccuStart II GelTrack® PCR SuperMix
It performance very well in my study!
We are a new lab, so still working through the different free samples that different vendors have given us. As of now, we have only finished evaluating different options for colony PCR and will get to the tough mixes later. The GelTrack SuperMix performed equally as well as any other polymerase we tested on E.coli colony PCR, where the colony itself is used as a template. It’s not a demanding template, so we were not able to discriminate based on performance or sensitivity…. all of them worked. Frankly, we are interested in this polymerase because you are the only company that keeps a reasonable price for the mastermix, while other companies more than double the price. I can afford to speed up my lab with a robust polymerase without needing to break the bank. Thank you for being a reasonable company …. it got our business.