Quantitative RT-qPCR comparison chart
| Quantitative RT-qPCR | |||||||||
| Probe-Based Detection | SYBR Green Detection | ||||||||
| eQo 1-Step ToughMix | Qscript lyo 1-Step | qScript XLT 1-Step RT-qPCR ToughMix | qScript One-Step RT-qPCR Kit | UltraPlex 1-Step ToughMix | qScript 1-Step Virus ToughMix | qScript One-Step SYBR Green RT-qPCR | |||
| Features | |||||||||
| Kit Format | 1 tube of Lyophilized Beads & Rehydration Buffer | One Tube | One Tube | Two Tubes | One Tube | One Tube | Two Tubes | ||
| RT Enzyme | qScript Ultra RT | MMLV, reduced RNase activity | MMLV, reduced RNase activity | MMLV, RNase H+ | MMLV, reduced RNase activity | MMLV, reduced RNase activity | MMLV, RNase H+ | ||
| Concentration | 4x | N/A | 2x | 2x | 4x | 2x | 2x | ||
| Yield | ++++ | ++++ | ++++ | +++ | ++++ | ++++ | +++ | ||
| Total RNA Input Range | 1pg – 100ng | 0.5pg – 500 pg | 1pg – 100ng | 1pg – 1ug | 1pg – 100ng | 1pg – 100ng | 1pg – 100ng | ||
| Amplicon Length | 70 – 150bp | 70 – 300bp | 70 – 300bp | 70 – 300bp | 70 – 300bp | 70 – 300bp | 70 – 200bp | ||
| Hot Start | Yes | N/A | N/A | N/A | Yes | Yes | N/A | ||
| Applications | |||||||||
| Inhibitor Tolerant | • | • | • | • | |||||
| High Yield | • | • | • | • | |||||
| Multiplex PCR | • | • | • | • | • | ||||
| Available Formats | |||||||||
| Master Mix | • Lyophilized Beads with Rehydration Buffer |
• | • | • | • | ||||
| Lyophilized | • | ||||||||
| Packaging (Rxns/Units) | 100 Rxns 1000 Rxns |
8 Rxns 24 Rxns |
100 Rxns 500 Rxns 2000 Rxns |
50 Rxns 200 Rxns |
100 Rxns 500 Rxns 1000 Rxns |
100 Rxns 500 Rxns 2000 Rxns |
100 Rxns 400 Rxns |
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