Extracta Plus RNA

Rapid extraction and purification of high-quality total RNA from cultured cells or tissue

Features & Benefits

  • Rapid Protocol – From cells or tissue samples to high-quality total RNA in just 25 mins
  • Streamlined gDNA removal – Novel DNA removal column eliminates gDNA contamination and need for DNase treatment
  • Extraction of low input quantities – Ability to extract RNA from as little as 10 cells
  • Downstream compatibility – Ideal for applications such as RT-PCR, RT-qPCR and next generation sequencing

Extracta Plus RNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

The Extracta Plus RNA kit provides rapid extraction and purification of RNA from cultured human or animal cells and from various tissue samples. The Extracta Plus spin column workflow enables simultaneous processing of multiple samples in 25 mins to yield purified RNA suitable for a range of downstream applications. The kit includes specially designed Extracta Plus DNA removal columns for effective removal of genomic DNA contamination. This provides a convenient solution for applications that are sensitive to low amounts of DNA contamination such as RT-qPCR and NGS.

Workflow

The efficient, reproducible extraction process allows total RNA extraction from as little as 10 cells input quantity.

Extracta Plus RNA complete workflow


Extracta Plus RNA complete workflow

This procedure can be completed in just 25 minutes, resulting in ready-to-use total RNA applicable for sensitive downstream applications such as RT-qPCR and NGS.


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  • Procedure can be completed in 25 minutes
  • Applicable for sensitive downstream applications such as RT-qPCR, RT-PCR and NGS


Reproducible, High Yield RNA

The efficient, reproducible extraction process allows total RNA extraction from as little as 10 cells input quantity.

Total RNA purification yield


Total RNA purification yield

Quantitative extraction of RNA from dilute cell quantities. Total RNA was purified in triplicate from HeLa cell quantities from as much as 100,000 to as little as 10 cells using the Extracta Plus RNA Kit and eluted in a volume of 30 µl. A portion of the RNA (1.5 µl) was then used for qPCR analysis of beta-actin mRNA using the qScript XLT ToughMix. Results demonstrate successful and reproducible extraction of RNA from a wide range of input.


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  • Total RNA purified from 100,000 to as little as 10 cells
  • RT-qPCR performed with β-actin mRNA using the qScript XLT ToughMix


Effective gDNA Removal

The Extracta DNA removal column achieves effective gDNA removal without a separate DNase treatment. Quantification of residual gDNA by qPCR demonstrates negligible gDNA within the total RNA extracted from cultured cells or tissues.

Effective RNA yield with negligible gDNA


Effective RNA yield with negligible gDNA

Total RNA was purified from (A) 1 x 106 HeLa cells or (B) 10 mg rat kidney tissue using the Extracta RNA Plus Kit. RT-qPCR assays were performed with (+RT) or without (-RT) reverse transcriptase.


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  • Total RNA purified from HeLa cells & rat kidney tissue
  • High total RNA yields, negligible gDNA


Amplification of Long mRNA Targets

Successful extraction of intact high molecular weight RNA was demonstrated by RT-PCR of long targets, ideal for cloning or long range sequencing applications.

Long mRNA target amplification


Long mRNA target amplification

Extraction of intact high molecular weight RNA from tissues and cultured cells. Total RNA was purified from either 5 or 10 mg of the rat kidney (K) or lung (L) tissue or from 1X106 cultured HeLa cells (H) using the Extracta RNA Plus Kit. Large fragments of the rat Dynein mRNA and the human SYNE1 mRNA were amplified via RT-PCR from 200 ng of RNA using qScript Ultra Flex Kit and then repliQa HiFi Toughmix. Gel analysis of the product demonstrates large quantities of intact and high molecular weight mRNA originally present in the RNA samples.


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  • Large quantities of intact and high molecular weight mRNA present
  • mRNA amplified via RT-PCR using the qScript Ultra Flex Kit and repliQa HiFi ToughMix


Additional Performance Data

Typical yields from common sources


Typical yields from common sources

Typical yields of total RNA using the Extracta RNA Plus Kit


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Effective gDNA removal


Total RNA yield & negligible gDNA yield

qPCR assays for human or rat β-actin were performed. A standard curve of human/rat gDNA was used to quantify the amount of gDNA contamination in the purified RNA. For all samples, gDNA was present in negligible quantities.


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